The Influence of Cytokinins and Auxins on Alstroemeria in Tissue Culture

Gabryszewska, E.; Hempel, M.

doi:10.17660/actahortic.1985.167.31

Cited by 13 publications

(12 citation statements)

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“…Most published methods on the micropropagation of Alstroemeria are based on shoot regeneration from rhizome apices (Gabryszewska & Hempel 1985;Lin & Monette 1987;Pedersen & Brandt 1992;Pedersen et al 1996;Kristiansen et al 1999;Chiari & Bridgen 2000). The problem accompanying this method concerns scarcity of initial explants and their heavier contamination.…”

Section: Discussionmentioning

confidence: 99%

“…Alstroemeria was propagated formerly by rhizome division but this method is not efficient and involves a high risk of viral dissemination (Van Zaayen 1995). Therefore, in vitro propagation techniques based on rhizome multiplication in vitro have been developed (Gabryszewska & Hempel 1985;Hakkaart & Versluijs 1988;Van Zaayen et al 1992;Bond & Alderson 1993). However, the multiplication rate was still not satisfactory (Lin et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base

Nasri¹,

Mortazavi²,

Ghaderi³

et al. 2013

Journal of Horticultural Research

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In the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) concentrations (0, 0.5, 1, 1.5 and 2 mg·1-1) and three indole-3-butyric acid (IBA) concentrations (0, 0.1 and 0.2 mg·1-1). The cultures were incubated at 21 ± 2 °C under photoperiod 16/8. After three subculturings (3 weeks-long each) the number of rhizome, shoots, buds, leaves, and roots, length of shoots and roots were recorded. Adventitious shoots formed directly on the leaf bases without callus intervention. Their number was affected by BAP concentrations. The highest shoots number, six per explants, was obtained at 1 mg·1-1 BAP and 0.1 mg·1-1 IBA. The shoot length decreased with the increasing concentration of BAP. The highest root number (2.7) was formed on shoots cultured on the MS medium containing 0.5 mg·1-1 α- naphthalene acetic acid, and the highest rhizome number (2.2) was formed on the medium with 0.5 mg·1-1 BAP. In vitro rooted plantlets were able to survive and acclimatize in the greenhouse.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base

Nasri¹,

Mortazavi²,

Ghaderi³

et al. 2013

Journal of Horticultural Research

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“…Most published methods for the micropropagation of Alstroemeria are based on plant regeneration from rhizome apices (Gabryszewska and Hempel, 1985;Lin and Monette, 1987;Pedersen and Brandt, 1992;Pedersen et al, 1996;Kristiansen et al, 1999;Chiari and Bridgen, 2000), in spite of the disadvantages when these explants are used: destruction of the mother plant, high contamination rate (Pedersen and Brandt, 1992;Hutchinson et al, 1997), and low multiplication rate, compared to other plants (Kristiansen et al, 1999). We found that explants dissected from aerial organs of Alstroemeria, such as leaf sections 1 cm 2 and 1 cm long stem apices, reached a good level of disinfection simply by immersion in sodium hypochlorite 1.8% v/v for 20 min.…”

Section: Discussionmentioning

confidence: 99%

“…On this regard, Hakkaart and Versluijs (1988) pointed out that the in vitro rooting of Alstroemeria can be induced without any plant hormones. However, other researchers have obtained their best rooting rates in the presence of NAA (Gabryszewska and Hempel, 1985;Pierik et al, 1988;Pedersen et al, 1996;Kristiansen et al, 1999).…”

Section: )mentioning

confidence: 98%

In vitro regeneration of Alstroemeria cv. ‘Yellow King’ by direct organogenesis

Pedraza‐Santos

López-Peralta

Hernández

et al. 2005

Plant Cell Tiss Organ Cult

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The common techniques for the in vitro production of Alstroemeria plants are based on rhizomes as explants, which have low multiplication rates and a high risk of carrying viral diseases. To overcome these problems, we developed a protocol for the in vitro regeneration of Alstroemeria cv.'Yellow King', by testing for shoot induction several explant sources (leaf, stem apices, rhizomes and immature inflorescence apices), temperature and light/dark regimes, hormone and salt concentrations. For shoot multiplication and rooting, several hormone concentrations were tested. We found that only the young floral apices produced adventitious shoots by direct organogenesis. The highest shoot induction rate (10.4 shoots per explant) was obtained by incubation in the dark for 15 days at 8°C followed by 15 days at 25°C and a 16-h/8-h light/ dark regime, on a Murashige and Skoog (1962) liquid medium at 50% of the salt concentration, supplemented with 2.5 mg l )1 KIN, 1.5 mg l )1 BA and 1.0 mg l )1 NAA, using a piece filter paper to support the explant. The highest shoot multiplication rate (9 shoots per explant) was obtained on a liquid MS medium at full strength supplemented only with BA at 1.0 mg l )1 . In vitro rooting of shoots was induced also on a liquid MS medium, either with or without plant hormones.

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“…Micropropagation was accomplished not only via the simple division of the initial explants but, especially, by using the cytokinin induced mass proliferation of daughter plants that retained the traits of the parental plant [42][43][44]. The application of new methods to stimulate growth and development by using appropriate sets of growth regulators [45,46], the proper choice of explant types, introduction of new chemicals [47], testing of antibiotics for control of bacterial diseases [48], all brought about spectacular achievements.…”

Section: Micropropagationmentioning

confidence: 99%

65 years of in vitro culture in Poland

Zenkteler

2013

Acta Soc Bot Pol

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This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements

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The Influence of Cytokinins and Auxins on Alstroemeria in Tissue Culture

Cited by 13 publications

References 0 publications

Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base

Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base

In vitro regeneration of Alstroemeria cv. ‘Yellow King’ by direct organogenesis

65 years of in vitro culture in Poland

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