The influence of dehydration on catalase stability. A comparison with freezing effects

Darbyshire, B.

doi:10.1016/0011-2240(74)90304-6

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…Ashwood-Smith and Warby (2) examined the addition of various cryoprotective agents in the inactivation of catalase during freezing and thawing. Similar studies were performed during dehydration (6). High-molecular-weight agents such as polyvinyl-pyrollidone were found to exert more protection in both of these studies.…”

supporting

confidence: 67%

“…Litchfield (17) has observed an inactivation of the catalase activity by the chloride ion. Acetate, a monovalent anion, also has been implicated in the inactivation of catalase during dehydration (6). Bovine serum albumin was utilized to examine the effects of protein on heat stability of catalase (data not presented).…”

Section: Figmentioning

confidence: 99%

“…High-molecular-weight agents such as polyvinyl-pyrollidone were found to exert more protection in both of these studies. Darbyshire (6) also reported a variation in catalase inactivation during dehydration using different buffers. Catalase in acetate buffer lost activity, whereas catalase in phosphate buffer exhibited no inactivation.…”

mentioning

confidence: 92%

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Heat inactivation of catalase from Staphylococcus aureus MF-31

Andrews¹,

Martin²

1979

Appl Environ Microbiol

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The effects of heat on catalase from Staphylococcus aureus lysates were examined. Catalase activity increased with increasing concentrations of potassium phosphate buffer, when heated at temperatures between 50 and 65°C for 10 min. Inactivation of catalase by NaCl during heating was demonstrated. Extended heating of S. aureus cells at 52°C resulted in a slight decrease in catalase activity of the resultant lysates. This decrease was more pronounced in the presence of salt. Heating at 62°C caused a decrease in catalase activity, but not complete inactivation. These results implicate the combined effects of heat and NaCl in the inactivation of catalase from S. aureus. The findings are consistent with the hypothesis that H202 may accumulate as a result of decreased catalase activity and be responsible for the decreased colony-forming ability of stressed S. aureus.

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supporting

confidence: 67%

Section: Figmentioning

confidence: 99%

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Heat inactivation of catalase from Staphylococcus aureus MF-31

Andrews¹,

Martin²

1979

Appl Environ Microbiol

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“…Polyvinylpyrrolidone fully protects both catalase and LDH. 39,90 Polyethylene glycol confers partial protection of β -galactosidase, partial or no protection of LDH, and no protection of phosphofructokinase. 18,30,45 Polyethylenimine partially protects LDH activity.…”

Section: Additives and Protection From Freeze-drying Stressmentioning

confidence: 99%

“…Slightly more data are available for protection by synthetic polymers. Polyvinylpyrrolidone fully protects both catalase and LDH. , Polyethylene glycol confers partial protection of β-galactosidase, partial or no protection of LDH, and no protection of phosphofructokinase. ,, Polyethylenimine partially protects LDH activity . Polidocanol is ineffective against freeze-drying. , Nevertheless, these studies are too limited to draw robust conclusions.…”

Section: Additives and Protection From Freeze-drying Stressmentioning

confidence: 99%

Protecting Enzymes from Stress-Induced Inactivation

Piszkiewicz

Pielak

2019

Biochemistry

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The pharmaceutical and chemical industries depend on additives to protect enzymes and other proteins against stresses that accompany their manufacture, transport, and storage. Common stresses include vacuum-drying, freeze-thawing, and freeze-drying. The additives include sugars, compatible osmolytes, amino acids, synthetic polymers, and both globular and disordered proteins. Scores of studies have been published on protection, but the data have never been analyzed systematically. To spur efforts to understand the sources of protection and ultimately develop more effective formulations, we review ideas about the mechanisms of protection, survey the literature searching for patterns of protection, and then compare the ideas to the data.

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Plant Water Relations

Lange

1975

Progress in Botany / Fortschritte Der Botanik

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The influence of dehydration on catalase stability. A comparison with freezing effects

Cited by 11 publications

References 12 publications

Heat inactivation of catalase from Staphylococcus aureus MF-31

Heat inactivation of catalase from Staphylococcus aureus MF-31

Protecting Enzymes from Stress-Induced Inactivation

Plant Water Relations

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