The influence of diet consistence, drinking water and bedding on periodontal disease in Sprague‐Dawley rats

Björnsson, Magnús Jón; Velschow, Sten; Stoltze, Kaj; Havemose-Poulsen, Anne; Schou, Søren; Holmstrup, Palle

doi:10.1034/j.1600-0765.2003.00678.x

Cited by 50 publications

(71 citation statements)

References 29 publications

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“…16 Therefore, ovariectomy-induced osteopenia in the rat produces skeletal responses similar to those in post-menopausal women. 17 A morphometric analysis of defleshed jaws [11][12][13]18,19 was applied to assess the amount of periodontal bone in the rats. This analysis is reproducible and can discriminate among experimental groups.…”

Section: Discussionmentioning

confidence: 99%

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

et al. 2011

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The purpose of this study was to morphometrically evaluate the influence of different durations of ovariectomy-induced estrogen deficiency on alveolar bone loss associated with ligature-induced bone loss in rats. Sixty female Wistar rats were randomly assigned to ovariectomy (OVX test group) or sham operation (SHAM control group). The OVX and SHAM groups were each distributed into three subgroups of ten rats each according to the duration of estrogen deficiency (30, 60 and 90 postoperative days). In all groups, for the last 30 days of the experimental period, cotton ligatures were placed around the cervix of the right upper second molar; the contralateral tooth was left unligated to serve as a control. The maxillary bones were removed, and the alveolar bone loss was analyzed by measuring the distance between the cementoenamel junction and the alveolar bone crest at the buccal site of the right upper second molar. A comparison between the ligated and unligated groups verified the presence of ligature-induced alveolar bone loss (p < 0.05). No significant differences were observed among the unligated groups (p > 0.05). A significant increase in bone loss was observed when ligation occurred 90 days after ovariectomy compared with the sham group (p < 0.05). These results demonstrate that long-term estrogen deficiency affects ligatureinduced alveolar bone loss.

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Section: Discussionmentioning

confidence: 99%

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

et al. 2011

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“…The total alveolar bone loss was obtained by taking the sum of the recordings from the buccal tooth surface and subtracting the value of the right maxilla (unligated control) from the left, in millimeters. Although it is claimed that laboratory rats may harbor ongoing baseline periodontitis (23), in the present study we used the contralateral right maxilla as the unligated control in order to avoid the influence of a baseline periodontitis on our results.…”

Section: Measurement Of Alveolar Bone Lossmentioning

confidence: 99%

Comparison of etoricoxib and indomethacin for the treatment of experimental periodontitis in rats

Azoubel¹,

Menezes²,

Bezerra³

et al. 2007

Braz J Med Biol Res

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We investigated the effect of etoricoxib, a selective cyclooxygenase-2 inhibitor, and indomethacin, a non-selective cyclooxygenase inhibitor, on experimental periodontitis, and compared their gastrointestinal side effects. A ligature was placed around the second upper left molars of female Wistar rats (160 to 200 g). Animals (6 per group) were treated daily with oral doses of 3 or 9 mg/kg etoricoxib, 5 mg/kg indomethacin, or 0.2 mL saline, starting 5 days after the induction of periodontitis, when bone resorption was detected, until the sacrifice on the 11th day. The weight and survival rate were monitored. Alveolar bone loss (ABL) was measured as the sum of distances between the cusp tips and the alveolar bone. The gastric mucosa was examined macroscopically and the periodontium and gastric and intestinal mucosa were examined by histopathology. The ongoing ABL was significantly inhibited (P < 0.05) by 3 and 9 mg/kg etoricoxib and by indomethacin: control = 4.08 ± 0.47 mm; etoricoxib (3 mg/kg) = 1.89 ± 0.26 mm; etoricoxib (9 mg/kg) = 1.02 ± 0.14 mm; indomethacin = 0.64 ± 0.15 mm. Histopathology of periodontium showed that etoricoxib and indomethacin reduced inflammatory cell infiltration, ABL, and cementum and collagen fiber destruction. Macroscopic and histopathological analysis of gastric and intestinal mucosa demonstrated that etoricoxib induces less damage than indomethacin. Animals that received indomethacin presented weight loss starting on the 7th day, and higher mortality rate (58.3%) compared to etoricoxib (0%). Treatment with etoricoxib, even starting when ABL is detected, reduces inflammation and cementum and bone resorption, with fewer gastrointestinal side effects.

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“…To avoid spontaneous PD (Bjornsson et al, 2003), the animals were bred on Teklad 7089 Diamond Soft Bedding (Harlan Laboratories, Livermore, CA, USA). The rat pups were placed on the special bedding before they were 15 days old, with free access to food (Purina #5008, LabDiet, St. Louis, MO, USA) and water.…”

Section: Methodsmentioning

confidence: 99%

“…Before PD was induced, all rats were examined to exclude rats with pre‐existing PD (defined by probing depths >0.5 mm) (Bjornsson et al, 2003). Each week, PD induction was checked under general anesthesia and loose or lost ligatures were replaced as previously described (17; 18).…”

Section: Methodsmentioning

confidence: 99%

“…Animal models with both T2D and PD have increased inflammatory cytokine responses, elevated oxidative stress, raised AGE levels, altered glucose metabolism, and increased severity of PD (Pontes Andersen, Flyvbjerg, Buschard, & Holmstrup, 2007). In addition to being a local irritant, the ligature serves as a reservoir for bacteria, which mediates periodontal tissue destruction (Bjornsson et al, 2003). Male Zucker obese rats (ZOR) display a mutation in their leptin receptors and will therefore develop obesity, hyperlipidemia and mild hyperglycemia.…”

Section: Introductionmentioning

confidence: 99%

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Ligature‐associated bacterial profiles are linked to type 2 diabetes mellitus in a rat model and influenced by antibody treatment against TNF‐α or RAGE

Grauballe

Belstrøm

Østergaard

et al. 2017

Clinical & Exp Dental Res

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There is a bidirectional relationship between periodontal disease (PD) and type 2 diabetes mellitus (T2D). T2D may lead to ecological perturbations in the oral environment, which may facilitate an altered microbiota. However, previous studies have been inconclusive in determining the effect of T2D on oral bacterial profiles. Therefore, we aimed to evaluate the influence of T2D on the ligature‐associated bacterial profile in a diabetic rat model with PD and investigated the impact of blocking inflammatory pathways with antibodies targeting either Tumor Necrosis Factor α (TNF‐α) or the receptor of advanced glycation end‐products (RAGE). A total of 62 Zucker obese rats (45 T2D) and 17 lean (non‐T2D) were divided into 4 treatment groups; lean with PD, obese with PD, obese with PD and anti‐TNF‐α treatment, and obese with PD with anti‐RAGE treatment. Periodontal disease was ligature induced. Ligature‐associated bacterial profiles were analyzed using Human Oral Microbe Identification Microarray (HOMIM). Ligature‐associated bacterial profiles differed between lean and obese rats. Furthermore, treatment with antibodies against TNF‐α or RAGE had an impact on subgingival bacterial profiles. T2D phenotypes are associated with different ligature‐associated bacterial profiles and influenced by treatment with antibodies against TNF‐α or RAGE.

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The influence of diet consistence, drinking water and bedding on periodontal disease in Sprague‐Dawley rats

Abstract: The results of the present study emphasize the need for pre- experimental examination of rats. They also show that diet and bedding conditions have the potential of seriously influencing outcomes of studies of periodontal disease in rats.

Cited by 50 publications

References 29 publications

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

Comparison of etoricoxib and indomethacin for the treatment of experimental periodontitis in rats

Ligature‐associated bacterial profiles are linked to type 2 diabetes mellitus in a rat model and influenced by antibody treatment against TNF‐α or RAGE

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