the atmosphere that have a diameter of 0.5-100 µm (Sin et al., 2007). Studying these airborne particles is the main goal of aerobiology, a practice that attempts to understand the spread of allergenic and other diseases in humans, animals, and plants in order to prevent their possible destructive effects (Lacey and West, 2006). Aerobiology involves different fields that intend to diagnose and remedy biological and environmental problems in disciplines such as medicine, meteorology, entomology, environmental studies, phytopathology, agriculture, and criminology (Dopazo et al., 2000; Brown, 2004). Anemophilous plants, especially grasses, gymnosperms, and some angiosperms, are the main sources of atmospheric pollen (Lacey and West, 2006). Pollen may contain tens of allergenic proteins and glycoproteins of different types. Pollen that reaches the human respiratory system can cause allergic rhinitis, allergic conjunctivitis, and allergic asthma in sensitive individuals by releasing the allergic protein in its structure (Tekat, 2002; Puc, 2003; Serbes and Kaplan, 2014). In order to help patients who complain of pollen allergies it is useful to detect the type and proportion of pollen grains in the atmosphere. Because pollen grains affect health, pollen calendars have been prepared worldwide for many years (