The influence of environmental conditions on the mycelial growth of Antrodia cinnamomea in submerged cultures

Yang, Fan-Chiang; Huang, Hong-Wei; Yang, Mei‐Sang

doi:10.1016/s0141-0229(03)00136-4

Cited by 62 publications

(41 citation statements)

References 5 publications

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“…Previous studies demonstrated that the triterpenoid biosynthesis of A. cinnamomea is significantly affected by several environmental factors, such as light, elicitor, substrate, pH value, and developmental stages [29][30][31]. The gene expression levels of Ac-mvd were the highest in the mycelia of A. cinnamomea on the 7th day of the submerged culture.…”

Section: Discussionmentioning

confidence: 93%

Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase inAntrodia cinnamomea

Lin

Shao

et al. 2016

MATEC Web of Conferences

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Abstract. Mevalonate pyrophosphate decarboxylase (MVD) plays important roles in triterpenoid biosynthesis via the mevalonate pathway. A novel MVD gene was isolated and identified in Antrodia cinnamomea (Ac-mvd). The fulllength cDNA contained an open reading frame with a length of 1,209 bp and encoded a 402-amino acid polypeptide with a molecular mass of 43.3 kDa and a theoretical pI of 8.23. As incubation time was prolonged for 28 days, the triterpenoid content in the mycelium gradually increased and reached 39.192 ± 2.025 mg/g; the triterpenoid content of the fruiting body grew in the hay of Cinnamomum kanehirae (ACFB-CK), was 49.391 ± 2.675 mg/g, which was significantly different from other samples (P < 0.05). qRT-PCR revealed that the highest expression levels of Ac-mvd in the mycelium of Antrodia cinnamomea were detected on the 7th day. The expression levels gradually decreased as culture time was extended from 14 days to 42 days.

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Section: Discussionmentioning

confidence: 93%

Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase inAntrodia cinnamomea

Lin

Shao

et al. 2016

MATEC Web of Conferences

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“…The cell production was due to the homogenization and heat transference when physical and chemical conditions are maintained homogenous and more oxygen becomes available (16). An agitation at 50 rpm was used to increase the production of biomass and polysaccharides by Phellinus linteus (10), while for the culture conditions of polypore Antrodia cinnamomea an agitation of 100 rpm increased the mycelial growth (26). Table 2 summarizes the effect of agitation over the specific growth velocity of P. tricholoma with carbon sources (glucose and lactose).…”

Section: Effect Of Agitation and Carbohydrate Concentration Over The mentioning

confidence: 99%

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Vieira¹,

Liebl²,

Tavares³

et al. 2008

Braz. J. Microbiol.

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Basidiomycete fungi of the Polyporus genus are a source of secondary metabolites which are of medicinal interest as antibacterial compounds. As these substances are produced in a small amount by the fungi, the study of the cultivation conditions in vitro that could possibly optimize their production seems of major importance. The effects of glucose and lactose, pH and agitation on biomass concentration and on the specific growth rate caused by the basidiomycete Polyporus tricholoma were investigated. The initial pH (4.5, 6.5 and 8.5) was autoregulated at pH 5.5, and the agitation increased the mycelial growth and the specific growth rate. The high concentration of carbon sources (4%) increased biomass production. The lactose concentration and the absence of agitation were determinant in the production of antibacterial metabolites. The characterization of the antibacterial substance by GC-MS indicated a major compound, isodrimenediol, produced by the fungus Polyporus tricholoma with activity against Staphylococcus aureus.

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“…Carbon sources are major components of the nutrient medium, which must ensure the best growth of fungi. Submerged culture has a number of advantages including higher mycelial yield in a more compact space and shorter time, with fewer chances of contamination [9][10][11] which is now attracting attention as an alternative for efficient yield of mycelia and polysaccharide [12]. The mycelial growth of edible mushroom using different carbon and nitrogen source under submerged culture condition was tested using basal synthetic media.…”

Section: Resultsmentioning

confidence: 99%

Growth of mycelial biomass and fruit body cultivation of Lentinus squarrosulus collected from home garden of Tripura in Northeast India

2015

J App Biol Biotech

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The present study aimed at cultivating the fruiting body of an edible mushroom identified as Lentinus squarrosulus collected from home garden of Tripura in Northeast India and for assessing the mycelium biomass growth using various carbon and nitrogen sources under submerged culture condition. The cultivation of the mushroom using paddy straw revealed dry weight of 0.25±0.02 g per fruit body with 21.83 % of biological efficiency. Under submerged culture condition, fructose exhibited highest mycelium dry weight and yeast extract was found to be a best nitrogen source. The availability of the mushroom in the forest and homegarden of Tripura may be utilized for its commercial cultivation and inclusion in the local food. Fructose and yeast extract is recommended for high mycelial biomass production.

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The influence of environmental conditions on the mycelial growth of Antrodia cinnamomea in submerged cultures

Cited by 62 publications

References 5 publications

Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase inAntrodia cinnamomea

Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase inAntrodia cinnamomea

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Growth of mycelial biomass and fruit body cultivation of Lentinus squarrosulus collected from home garden of Tripura in Northeast India

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