The Influence of Estrogens, Progesterone and their Antagonists on the Collagen Content in the Pregnant Rat Uterus

Bieńkiewicz, Andrzej; Welfel, Jakub; Kuś, Ewa

doi:10.1055/s-2007-979859

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…The same phenomenon can be confirmed by the immunohistochemical results, which reveal the reduced intensity of immunostaining in all layers of the uterus as the end of the gestational period approached. Considering the fact that rat uterine tissue has a low connective tissue content (less than 6%) and the hormonal changes towards the end of pregnancy furtherly reduce its amount (Bieńkiewicz et al, 1996), it can be stated that the connective tissue mass is marginal as compared with that of smooth muscles. Since no data is available about the Kiss1r expression in any connective tissue (and its probability is very low), we suppose that the immunostaining revealed the Kiss1r expression in the smooth muscle of the non-pregnant and pregnant rat uteri.…”

Section: Discussionmentioning

confidence: 99%

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

Schaffer

Hajagos-Tóth

Ducza

et al. 2021

European Journal of Pharmacology

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The objectives of this study were to investigate the effects of KISS1 94-121 fragment on the contractility of nonpregnant and pregnant rat uteri, and to determine the uterine and myometrial expressions of Kiss1r. Uterine muscle strips were obtained from non-pregnant Sprague-Dawley rats in oestrous phase and from pregnant rats on gestational days 5, 15, 18, 20 or 22. The in vitro contractility measurements were carried out in an isolated organ bath in the presence of KISS1 94-121. Experiments with 5-day pregnant tissues were also performed in the presence of kisspeptin-234 trifluoroacetate. The mRNA and protein expressions of Kiss1r were measured by RT-PCR and Western blot analysis, while localizations of receptors were defined by fluorescent immunohistochemistry. KISS1 94-121 induced a dose-dependent relaxation both in non-pregnant and pregnant intact and endometrium-denuded uteri. A gradual decrease was found in the uterine expressions of Kiss1r mRNA and protein towards the end of the gestational period, and it was further confirmed by the immunohistochemical results. The significant majority of Kiss1r is found in the myometrium, however the few endometrial Kiss1r also influences the uterine contractions. The relaxing effect of kisspeptin is continuously reduced towards the end of gestational period in parallel with the reduction of Kiss1r expression. Our results suggest a putative role of kisspeptin in the maintenance of uterine quiescence that may have significance in miscarriage or preterm contractions.

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Section: Discussionmentioning

confidence: 99%

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

Schaffer

Hajagos-Tóth

Ducza

et al. 2021

European Journal of Pharmacology

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“…In a complex research project we are currently investigating various influences affecting the physiology of the uterine cervix. Among these influences, the effects of hormones on the supply of blood, oxygen and water in the cervix is an important focus [4]. We recently demonstrated that FD-NIRS technology is highly sensitive and accurate in detecting changes in cervical physiology [3,10].…”

Section: Discussionmentioning

confidence: 99%

“…We recently demonstrated that prostaglandininduced cervical ripening results in a significant change in optical properties (i.e., the absorption and scattering of light), a significant decrease in total hemoglobin (tHb), oxyhemoglobin (O 2 Hb), deoxyhemoglobin (HHb), and a highly significant increase in water content (H 2 O) [3]. So far, the influence of sex hormones on the optical properties of the human uterine cervix has not been investigated, but it is likely to be important in the process of cervical ripening [4].…”

Section: Introductionmentioning

confidence: 99%

Optical properties, physiologic parameters and tissue composition of the human uterine cervix as a function of hormonal status

Matzinger

Wolf²,

Baños

et al. 2008

Lasers Med Sci

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The influence of sex hormones on the human uterine cervix is likely to be important in the process of cervical ripening. Frequency domain near-infrared spectroscopy (FD-NIRS) was used to investigate non-invasively the changes in the optical properties that reflect physiologic parameters and tissue composition of the uterine cervix in the different phases of the menstrual cycle. Twenty premenopausal and nine postmenopausal women were examined. Optical properties of the uterine cervix were measured, and physiological parameters [concentration of water, oxyhemoglobin (O(2)Hb) and deoxyhemoglobin (HHb), total hemoglobin (tHb), oxygen saturation (StO(2)), water, and scattering power] were calculated. Analysis of variance (ANOVA) was used to test for statistical significance. The optical properties of the anterior cervical lip did not differ from those of the posterior lip. HHb was significantly lower in cervices during menstrual bleeding than during the follicular, luteal, or postmenopausal phases. The ratio of O(2)Hb to HHb was highly significantly increased by a factor of 2 when cervices during the menstrual bleeding were compared with those during the follicular, luteal, or postmenopausal phases. The scattering power was significantly lower during menstrual bleeding than during the follicular or postmenopausal phases. We demonstrated that withdrawal of sex hormones during menstrual bleeding is associated with a significant decrease in HHb and scattering power, with stable values of O(2)Hb, tHb, StO(2), and H(2)O compared with the values during the follicular, luteal or postmenopausal phases of the menstrual cycle. Cervical softening during menstrual bleeding seems to be different from cervical softening for labor.

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“…Finally, regarding the results of immunohistochemistry, it should be clarified that the methods used in this study were not suitable for quantitative measurements. However, considering the fact that rat uterus has a low connective tissue content and its amount is further reduced due to hormonal changes towards the end of pregnancy 84 , it can be stated that the amount of connective tissue is marginal as compared with that of smooth muscle. Generally, we can state that immunostaining revealed the adipokine receptor expressions in the smooth muscle of the non-pregnant and pregnant rat uteri.…”

Section: Discussionmentioning

confidence: 99%

The ontogeny of kisspeptin, leptin and adiponectin

Schaffer

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The Influence of Estrogens, Progesterone and their Antagonists on the Collagen Content in the Pregnant Rat Uterus

Cited by 6 publications

References 5 publications

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

Optical properties, physiologic parameters and tissue composition of the human uterine cervix as a function of hormonal status

The ontogeny of kisspeptin, leptin and adiponectin

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