The influence of ethanol extract Dioscorea alata L. on CD4+CD62L+ and CD8+CD62L+ profile of BALB/c mice model digestive tract allergy

Makiyah, Sri Nabawiyati Nurul; Widodo, Widodo; Rifa’i, Muhaimin; Djati, Muhamad Sasmito

doi:10.1063/1.4953528

Cited by 3 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it has not been much utilized optimally (Christina & Rifa'i, 2014). Our previous research proved that an ethanol extract of D. alata had hypo-allergenic and immuno-modulatory activity (Makiyah, 2018;Makiyah et al, 2014Makiyah et al, , 2016Makiyah et al, , 2015. Steroidal saponin is the most crucial compounds contained in Dioscorea species because it has some biological functions such as immunomodulatory and antiinflammatory depend on its structure (Dong et al, 2008;Kaur et al, 2021;Wang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin

et al. 2023

View full text Add to dashboard Cite

Steroidal saponin compound in Dioscorea species is one of the crucial substance because it has some biological functions, such as immunomodulatory and anti-allergic agent. This study aims to analyze the effect of steroidal saponin of Dioscorea alata L. on CD4 T cells activation, cytokines IL-4, IL-5 and TGF-β. This study used primary lymphocyte cell which isolated from BALB/c mice spleen. Mice lymphocyte were cultured in the complete medium with presence of various concentrations (50, 25 and 12.5 µg/mL) of three isolate of steroidal saponin of D. alata for 24 h. Diosgenin was used as positive control. After 24 h of treatment, the activation of lymphocyte, IL-4 and IL-5 production was determined by Flowcytometry analysis. Data were analyzed using student t-test with p < 0.05 and p < 0.01. The results showed that the low concentration of Isolate 1 (12.5 µg/mL) was found to be effective to stimulate the activation of CD4 T cells, reduce the production of IL4 and IL-5 by CD4 T cells and increase TGF-β production. These results showed that a low concentration of steroidal saponin of D. alata could be considered as immunomodulatory and anti-allergic agent. Purification and identification of each isolate isolated from DAE is in progress.

show abstract

Section: Introductionmentioning

confidence: 99%

Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In addition, research on D. alata L. is concerned with the activity of scavenging reactive oxygen species, antioxidants, antidiabetics, anticlastogenic, antiosteoporotic, hypoallergenic, and immunomodulatory activities. [1][2][3][4][5][6][7] However, D. alata L. is used as food and traditional medicine empirically from generation to generation. Based on the development of knowledge and research, it is necessary to know the safety of traditional medicine to avoid unwanted harmful or toxic effects, such as toxicity effect.…”

mentioning

confidence: 99%

In Silico Toxicity Prediction of Bioactive Compounds of Dioscorea alata L.

2022

TJNPR

View full text Add to dashboard Cite

Yam (Dioscorea alata L.) comes from the Dioscoreaceae family. This type of yam is native to Africa and Asia. It is a staple in tropical countries. In addition, D. alata L. is also widely cultivated to be used as a basic substance for traditional medicine. D. alata L. has long been known to contain diosgenin. 1 Diosgenin is the most important steroidal saponin compound from D. alata L. because it has several biological functions, including immunomodulatory activity. D. alata L. has been shown to regulate metabolism, improve heart function, and have restorative effects. It has been shown to reduce fat in the liver and repair the cecum and colon after a high-fat diet. In addition, research on D. alata L. is concerned with the activity of scavenging reactive oxygen species, antioxidants, antidiabetics, anticlastogenic, antiosteoporotic, hypoallergenic, and immunomodulatory activities. [1][2][3][4][5][6][7] However, D. alata L. is used as food and traditional medicine empirically from generation to generation. Based on the development of knowledge and research, it is necessary to know the safety of traditional medicine to avoid unwanted harmful or toxic effects, such as toxicity effect. The toxicity effects include human hepatotoxicity (H-HT), drug-induced liver injury (DILI), carcinogenicity, immunotoxicity, mutagenicity, and cytotoxicity. Hepatotoxicity is defined as an injury to the liver whereas DILI is an acute or chronic response to a natural or manufactured compound.

show abstract

The Benefits of Uwi Plant (Dioscorea alata L.) for Health: Knowledge, Attitude, and Its Distribution in Daerah Istimewa Yogyakarta

Nabawiyati Nurul Makiyah,

Kamal,

Kita

et al. 2024

E3S Web Conf.

View full text Add to dashboard Cite

Uwi (Dioscorea alata L.) is a plant rich in health benefits, but many people in the Special Region of Yogyakarta (SRY) still do not realize it. This study aims to assess the level of knowledge, attitudes, and level of knowledge about the benefits for health and the distribution of uwi plants in the SRY. This is descriptive-observational research with a cross-sectional design, 578 respondents were taken by accidental sampling and non-random selection. This research used a questionnaire as an instrument. Data were analyzed descriptively. The results showed that 578 respondents had an average score of knowledge level of 86.34 ± 13.96 with a very good category, score of attitudes of 69.76 ± 11.37 and score of knowledge level of health benefits of 66.42 ± 12.57 with a fairly good category. Related to the distribution of uwi plants in the SRY, it was found that 86 respondents (14.88%) had uwi plants in their homes. It can be concluded that people in the Special Region of Yogyakarta have a very good level of knowledge about uwi plants, and have good attitudes and knowledge about the health benefits of uwi plants, but the distribution of uwi plants is still limited in this area.

show abstract

The influence of ethanol extract Dioscorea alata L. on CD4+CD62L+ and CD8+CD62L+ profile of BALB/c mice model digestive tract allergy

Cited by 3 publications

References 35 publications

Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin

Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin

In Silico Toxicity Prediction of Bioactive Compounds of Dioscorea alata L.

The Benefits of Uwi Plant (Dioscorea alata L.) for Health: Knowledge, Attitude, and Its Distribution in Daerah Istimewa Yogyakarta

Contact Info

Product

Resources

About