The influence of external environment fluctuations on the signal formation of microbiosensors

Аракелян, В. Б.; Wild, James R.; Simonian, Aleksandr

doi:10.1016/s0956-5663(00)00127-5

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…Different approaches of enzyme-based biosensors have been reported in the literature, regarding the immobilization techniques and the transducer systems. The importance of the detection of toxic materials in the environment, such as OP compounds, the components of several pesticides, − has been discussed and evaluated.…”

Section: Introductionmentioning

confidence: 99%

Langmuir and Langmuir−Blodgett Films of Organophosphorus Acid Anhydrolase

et al. 2003

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In this paper, we describe the preparation and characterization of Langmuir and Langmuir-Blodgett (LB) monolayers of the enzyme organophosphorus acid anhydrolase (OPAA). Langmuir films of OPAA were characterized on different subphases, such as phosphate, ammonium carbonate, and bis-tris-propane buffers. Monolayers at the air-water interface were characterized by measuring the surface pressure and surface potential-area isotherms. In situ UV-vis absorption spectra were also recorded from the Langmuir monolayers. The enzyme activity at the air-water interface was tested by the addition of diisopropylfluorophosphate (DFP) to the subphase. LB films of OPAA were transferred to mica substrates to be studied by atomic force microscopy. Finally, a one-layer LB film of OPAA labeled with a fluorescent probe, fluorescein isothiocyanate (FITC), was deposited onto a quartz slide to be tested as sensor for DFP. The clear, pronounced response and the stability of the LB film as a DFP sensor show the potential of this system as a biosensor.

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Section: Introductionmentioning

confidence: 99%

Langmuir and Langmuir−Blodgett Films of Organophosphorus Acid Anhydrolase

et al. 2003

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“…Brooks et al developed a microsensor in which antibodies were discretely immobilized on a variety of surfaces, thereby ensuring a segregation of the biorecognition elements [196]. Arakelian et al investigated the influence of the external environment on microbiosensor performances [197], and Xie et al reviewed the use of micro-thermal transducers and mini-channel and multi-channel systems [198].…”

Section: Microbiosensors and Nanobiosensorsmentioning

confidence: 99%

Biosensors for environmental pollutants and food contaminants

Baeumner

2003

Analytical and Bioanalytical Chemistry

216

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This review article provides an overview of the most recent literature on biosensors for environmental pollutants and food contaminants. Due to the large number of publications, only papers published between 2000 and January 2003 were considered. Also, while not all of the published literature could be reviewed here, over 200 references are cited to provide a good overview of research undertaken in the last two years. Older publications are covered by a number of earlier review articles. This article provides an introduction into the field including specific consideration of the application areas, describes the typical biosensor assay format used, and is subsequently structured according to the biorecognition elements used (i.e., nucleic acids, enzymes, whole cells, tissue and whole organisms, antibodies and receptors, and biomimetic materials). In addition, a section on microbiosensing systems is provided. Since only very few microbiosensors with applications in environmental and food systems have been published, enabling technology is also covered in this article.

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Adsorption of Ligands on Macromolecules in the Fluctuating Medium

Arakelyan¹,

Haroutiunian²,

Arakelyan³

et al. 2002

Journal of Biomolecular Structure and Dynamics

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In the present work fluctuations of number of ligands adsorbed on macromolecule are investigated. We have taken into account the adsorption and desorption of ligands under the circumstance of some adsorption centers fluctuations affected by medium fluctuation. The correlation function and spectral density of number of ligands adsorbed on macromolecule are calculated. The properties of these fluctuations which allow identifying a noisemaker are determined. It has been shown, thatfas andsluggis adsorption can be distinguished by properties of dispersion and spectral density. It has been also shown, that comparison of experimental and theoretical correlation functions (or spectral densities) allows to calculate constants of ligand - adsorption center binding and unbinding.

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The influence of external environment fluctuations on the signal formation of microbiosensors

Cited by 3 publications

References 4 publications

Langmuir and Langmuir−Blodgett Films of Organophosphorus Acid Anhydrolase

Langmuir and Langmuir−Blodgett Films of Organophosphorus Acid Anhydrolase

Biosensors for environmental pollutants and food contaminants

Adsorption of Ligands on Macromolecules in the Fluctuating Medium

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