The influence of Gelatin/PCL ratio and 3-D construct shape of electrospun membranes on cartilage regeneration

Zheng, Rui; Duan, Huanan; Xue, Jixin; Liu, Yu; Feng, Bei; Zhao, Shifang; Zhu, Yonglin; Liu, Yi; He, Aijuan; Zhang, Wenjie; Liu, Wei; Cao, Yilin; Zhou, Guangdong

doi:10.1016/j.biomaterials.2013.09.082

Cited by 153 publications

(125 citation statements)

References 40 publications

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“…37 As described in the "Methods" section, a catheter was used to support the shape of the scaffold. Following implantation, the construct received most of its nutrients from the surface-connected skin and not from the inner surface of the tube.…”

Section: Discussionmentioning

confidence: 99%

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Fu¹,

Liu²,

Feng³

et al. 2014

IJN

Self Cite

212

117

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Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-ε-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young’s modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Fu¹,

Liu²,

Feng³

et al. 2014

IJN

Self Cite

212

117

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“…Gelatin, a natural biopolymer derived from partial hydrolysis of native collagen, has many integrin-binding sites for cell adhesion and differentiation [21]. PCL-gelatin hybrid material, a new biomaterial with good biocompatibility and improved mechanical, physical, and chemical properties [22], has been successfully used in various tissue engineering applications [23][24][25]. However, phase separation between PCL and gelatin adversely affect both the electrospinning process and the resultant fiber performance.…”

Section: Introductionmentioning

confidence: 99%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

330

219

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Email address: sharell@126.com (R. Shi). AbstractInfection is the major reason for guided tissue regeneration/guided bone regeneration (GTR/GBR) membrane failure in clinical application. In this work, we developed GTR/GBR membranes with localized drug delivery function to prevent infection by electrospinning of poly(ε-caprolactone) (PCL) and gelatin blended with metronidazole (MNA). Acetic acid (HAc) was introduced to improve the miscibility of PCL and gelatin to fabricate homogeneous hybrid nanofiber membranes. The effects of the addition of HAc and the MNA content (0,1,5,10,20,30, and 40 wt.% of polymer) on the properties of the membranes were investigated. The membranes showed good mechanical properties, appropriate biodegradation rate and barrier function. The controlled and sustained release of MNA from the membranes significantly prevented the colonization of anaerobic bacteria. Cells could adhere to and proliferate on the membranes without cytotoxicity until the MNA content reached 30%.Subcutaneous implantation in rabbits for 8 months demonstrated that MNA-loaded membranes evoked a less severe inflammatory response depending on the dose of MNA than bare membranes. The biodegradation time of the membranes was appropriate for tissue regeneration. These results indicated the potential for using MNA-loaded PCL/gelatin electrospun membranes as anti-infective GTR/GBR membranes to optimize clinical application of GTR/GBR strategies.

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“…The components and structure of scaffolds determine their mechanical properties, biocompatibility, and degradability, and thus inevitably influence the efficacy of 3D cartilage regeneration. 13,14 As shown by previous studies, natural, biodegradable materials including polylactic acid (PLA), polyglycolic acid (PGA), and PGA-PLA copolymer have good biocompatibility and biosafety. 15 However, poor mechanical strength and rapid degradability greatly limit their application in regenerating 3D tissue.…”

Section: 11mentioning

confidence: 99%

Chondrogenic Regeneration Using Bone Marrow Clots and a Porous Polycaprolactone-Hydroxyapatite Scaffold by Three-Dimensional Printing

Yao

Wei

Liu

et al. 2015

Tissue Engineering Part A

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Scaffolds play an important role in directing three-dimensional (3D) cartilage regeneration. Our recent study reported the potential advantages of bone marrow clots (MC) in promoting extracellular matrix (ECM) scaffold chondrogenic regeneration. The aim of this study is to build a new scaffold for MC, with improved characteristics in mechanics, shaping, and biodegradability, compared to our previous study. To address this issue, this study prepared a 3D porous polycaprolactone (PCL)-hydroxyapatite (HA) scaffold combined with MC (Group A), while the control group (Group B) utilized a bone marrow stem cell seeded PCL-HA scaffold. The results of in vitro cultures and in vivo implantation demonstrated that although an initial obstruction of nutrient exchange caused by large amounts of fibrin and erythrocytes led to a decrease in the ratio of live cells in Group A, these scaffolds also showed significant improvements in cell adhesion, proliferation, and chondrogenic differentiation with porous recanalization in the later culture, compared to Group B. After 4 weeks of in vivo implantation, Group A scaffolds have a superior performance in DNA content, Sox9 and RunX2 expression, cartilage lacuna-like cell and ECM accumulation, when compared to Group B. Furthermore, Group A scaffold size and mechanics were stable during in vitro and in vivo experiments, unlike the scaffolds in our previous study. Our results suggest that the combination with MC proved to be a highly efficient, reliable, and simple new method that improves the biological performance of 3D PCL-HA scaffold. The MC-PCL-HA scaffold is a candidate for future cartilage regeneration studies.Introduction D ue to the poor regenerative capacity of cartilage in vivo, articular cartilage defect is a great challenge in the field of orthopedic surgery.1 There are several cartilage repair techniques, including microfracture, joint irrigation or debridement, osteochondral grafting, and autologous chondrocyte implantation.2,3 As it is a simple, convenient, and relatively inexpensive choice, microfracture has become the preferred clinical treatment. 4 Previous evidence proved that bone marrow clots (MC) on the surface of the microfracture lesions provide an optimal environment for cartilaginous tissue repair. 5,6 However, this technique results in a repaired tissue with lower mechanical strength. This tissue is easily worn by daily activities, causing symptoms to recur and necessitating a second repair. 7,8 The poor mechanical strength, the instability of MC, and the loss of bone marrowderived mesenchymal stem cell (BMSCs) may be the main barriers to fibrocartilage repair by microfracture. 9 Although the detailed mechanisms by which MC fibrocartilage forms are not clear, the combination of MC chondrogenic differentiation and high-strength biodegradable scaffolds may provide an advanced approach to the repair and functional reconstruction of cartilage defects. 10,11The fundamental concept underlying tissue engineering is the combination of a scaffold with living cells and/o...

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The influence of Gelatin/PCL ratio and 3-D construct shape of electrospun membranes on cartilage regeneration

Cited by 153 publications

References 40 publications

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Chondrogenic Regeneration Using Bone Marrow Clots and a Porous Polycaprolactone-Hydroxyapatite Scaffold by Three-Dimensional Printing

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