The influence of humus acids of peloids and its components on free radical processes

Аввакумова, Н. П.; Kamilov, F H; Жданова, А В; Zhernov, Yury; Кривопалова, М А; Глубокова, М. Н.; Katunina, Elena E.

doi:10.18097/pbmc20186405429

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…The source for HA used in this study was the silt sulphide mud, which is used for medicinal purposes. [1,2] Aqueous HA cream (5%) produced by blending appropriate quantities of HA and additional water into emulsifying ointment BP. For induction of ACD-like skin disorders, DNCB was applied onto the BALB/c mouse dorsal skin.…”

Section: Methodsmentioning

confidence: 99%

Application of topical peloid-derived humic acid suppresses allergic contact dermatitis in mice model

Zhernov

Жданова

Аввакумова

et al. 2020

World Allergy Organization Journal

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Section: Methodsmentioning

confidence: 99%

Application of topical peloid-derived humic acid suppresses allergic contact dermatitis in mice model

Zhernov

Жданова

Аввакумова

et al. 2020

World Allergy Organization Journal

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“…The interaction of magnesium ions with HAs has a donor-acceptor and ion-ionic mechanism [18]. The mass fraction of magnesium ions in the complex with humic acids decreases more than twice as compared to the initial content.…”

Section: Main Bodymentioning

confidence: 97%

Study of the method of detoxification of mineralised water sediments by natural sorbents

Nikitina,

Tihonova,

Danilova

et al. 2023

From Chemistry Towards Technology Step-by-Step

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Sediments after reagent treatment of mineralised water are a rather complex mixture of various substances of mineral and organic composition. It is necessary to recycle these sediments in order to reduce the negative impact on the environment. The article considers the possibility of sediments detoxification by natural sorbents to bind organic and inorganic toxicants into non-toxic complexes and reduce the negative effect on the environment.

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“…There are suggestions that humic substances have a mechanism of immunostimulating activity due to the presence of organic nitrogen, which is capable of inducing cytokines and activating cells of the immune system [64]. This group of substances has a previously described broad biological activity [64][65][66][67][68][69], which makes it promising for testing in a potential antimelanoma agent screening model. There is evidence that lignins can influence the activation of the immune response in vitro.…”

Section: Obtaining Fractions Of Humic Substances and Chitosanmentioning

confidence: 99%

Determination of Common microRNA Biomarker Candidates in Stage IV Melanoma Patients and a Human Melanoma Cell Line: A Potential Anti-Melanoma Agent Screening Model

Antonova,

Hambikova,

Shcherbakov

et al. 2023

IJMS

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MicroRNAs (miRNAs) are small, non-coding RNAs that play an important role in regulating gene expression. Dysregulation of miRNA expression is commonly observed in cancer, and it can contribute to malignant cell growth. Melanoma is the most fatal type of skin malignant neoplasia. Some microRNAs can be prospective biomarkers for melanoma in stage IV (advanced) at higher risk of relapses and require validation for diagnostic purposes. This work aimed to (1) determine the most significant microRNA biomarker candidates in melanoma using content analysis of the scientific literature, (2) to show microRNA biomarker candidates’ diagnostic efficacy between melanoma patients and healthy control groups in a small-scale preliminary study by blood plasma PCR analysis, (3) to determine significant microRNA markers of the MelCher human melanoma cell line, which are also detected in patients with melanoma, that can be used as markers of drug anti-melanoma activity, and (4) test anti-melanoma activity of humic substances and chitosan by their ability to reduce level of marker microRNAs. The content analysis of the scientific literature showed that hsa-miR-149-3p, hsa-miR-150-5p, hsa-miR-193a-3p, hsa-miR-21-5p, and hsa-miR-155-5p are promising microRNA biomarker candidates for diagnosing melanoma. Estimating microRNA in plasma samples showed that hsa-miR-150-5p and hsa-miR-155-5p may have a diagnostic value for melanoma in stage IV (advanced). When comparing ΔCt hsa-miR-150-5p and ΔCt hsa-miR-155-5p levels in melanoma patients and healthy donors, statistically significant differences were found (p = 0.001 and p = 0.001 respectively). Rates ΔCt were significantly higher among melanoma patients (medians concerning the reference gene miR-320a were 1.63 (1.435; 2.975) and 6.345 (4.45; 6.98), respectively). Therefore, they persist only in plasma from the melanoma patients group but not in the healthy donors group. In human wild-type stage IV melanoma (MelCher) cell culture, the presence of hsa-miR-150-5p and hsa-miR-155-5p in supernatant was detected. The ability of humic substance fractions and chitosan to reduce levels of hsa-miR-150-5p and hsa-miR-155-5p was tested on MelCher cultures, which is associated with anti-melanoma activity. It was found that the hymatomelanic acid (HMA) fraction and its subfraction UPLC-HMA statistically significantly reduced the expression of miR-150-5p and miR-155-5p (p ≤ 0.05). For the humic acid (HA) fraction, this activity was determined only to reduce miR-155-5p (p ≤ 0.05). Ability to reduce miR-150-5p and miR-155-5p expression on MelCher cultures was not determined for chitosan fractions with a molecular weight of 10 kDa, 120 kDa, or 500 kDa. Anti-melanoma activity was also determined in the MTT test on MelCher cultures for explored substances. The median toxic concentration (TC50) was determined for HA, HMA and UPLC-HMA (39.3, 39.7 and 52.0 μg/mL, respectively). For 10 kDa, 120 kDa, or 500 kDa chitosan fractions TC50 was much higher compared to humic substances (508.9, 6615.9, 11352.3 μg/mL, respectively). Thus, our pilot study identified significant microRNAs for testing the in vitro anti-melanoma activity of promising drugs and melanoma diagnostics in patients. Using human melanoma cell cultures gives opportunities to test new drugs on a culture that has a microRNA profile similar to that of patients with melanoma, unlike, for example, murine melanoma cell cultures. It is necessary to conduct further studies with a large number of volunteers, which will make it possible to correlate the profile of individual microRNAs with specific patient data, including the correlation of the microRNA profile with the stage of melanoma.

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The influence of humus acids of peloids and its components on free radical processes

Cited by 4 publications

References 3 publications

Application of topical peloid-derived humic acid suppresses allergic contact dermatitis in mice model

Application of topical peloid-derived humic acid suppresses allergic contact dermatitis in mice model

Study of the method of detoxification of mineralised water sediments by natural sorbents

Determination of Common microRNA Biomarker Candidates in Stage IV Melanoma Patients and a Human Melanoma Cell Line: A Potential Anti-Melanoma Agent Screening Model

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