The Influence of Injection Conditions and Soil Types on Soil Improvement by Microbial Functions

Inagaki, Yukiko; Tsuji, Masayoshi; Mori, Hiroyuki; Sasaki, Tetsuya; Soga, Kenichi; Qabany, Ahmed Al; Hata, Toshiro

doi:10.1061/41165(397)411

Cited by 15 publications

(11 citation statements)

References 2 publications

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“…Stocks-Fischer [35] reported that the bacterial suspension density acts as nucleation sites for calcite precipitation processes, and this was confirmed [95]. The increase in the concentration of microbes has been reported [109] to be proportional to the amount of precipitated CaCO 3 , as long as it does not exceed the pore volume. Also, higher bacterial suspension density delivered into the soil leads to an increase in the MICP process [110].…”

Section: Availability Of Nucleation Sites (Bacterial Suspension Density)mentioning

confidence: 81%

“…Dejong [31] reported that both the nutrients and cementation reagents can rapidly be exhausted due to: (1) the flow rates through the soil medium being too fast for reaction to occur, (2) nutrients not being provided in sufficient quantity, or (3) nutrients being exhausted over time. To overcome some of these challenges, studies [4,20,24,109,195] have been conducted to determine or optimize the quantities of injection of the microbes, nutrients, and cementation reagents. Some of the reported findings of the studies are: (1) The stop-flow injection method is preferred over continuous and recirculation methods of injection, as it provides an even and more uniform distribution of calcite when compared with the other two methods, which either result in greater filling of voids near the injection points or flushing of some of the microbes, preventing their participation in the precipitation process; (2) A maximum of two-thirds of the pore volume should be used as the injection volume for the microbes, nutrients, and cementation reagents [24]: (3) It is recommended [24] that a maximum of one-third of the pore volume should be used for the injection of microbes, an approach that yielded positive results with tropical residual soils [113][114][115].…”

Section: Nonuniform Injection Of Microbes and Cementation Reagentsmentioning

confidence: 99%

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Review of the use of microorganisms in geotechnical engineering applications

et al. 2020

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Until recently, engineers either ignored or neglected the role of microorganisms in geotechnical engineering. The microbially induced calcite precipitation (MICP) research technique is an innovative and relatively green technology which consists in a biological process through which microorganisms react with minerals (calcium source and cementation reagent) to produce calcite (CaCO 3 ) as a byproduct that modifies and improves the engineering properties of soil. Laboratory and field results obtained from various studies are promising and viable, suggesting potential for various engineering applications. This research technique has also been considered to be useful in many engineering applications such as improvement of construction materials, cementation of porous media, improvement in strength and stiffness of engineering soils, hydraulic control of engineering facilities (waste containment), liquefaction, and erosion mitigation. In this review, the various methods of production of calcium carbonates (calcite), the role played by bacteria, various state-of-the-art procedures that have evolved in this research area, as well as ongoing studies are reported. Furthermore, the use of the MICP technique in remediation of contaminants and other environmental concerns is also presented. The advantages and challenges (in form of undesired byproducts) of this research technique are highlighted herein.

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Section: Availability Of Nucleation Sites (Bacterial Suspension Density)mentioning

confidence: 81%

Section: Nonuniform Injection Of Microbes and Cementation Reagentsmentioning

confidence: 99%

Review of the use of microorganisms in geotechnical engineering applications

et al. 2020

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“…Each specimen was treated with MICP or BEICP to compare the impact of both treatment methods on UCS and permeability. Soil specimens were treated with either 8-12-, or 16-cycles, given that Inagaki et al (2011) and Choi et al (2016) used similar levels of ICP cycling. More detailed information regarding the treatment methodologies will be provided in the next section.…”

Section: Soil Stabilization Column Preparationmentioning

confidence: 99%

Sand and silty-sand soil stabilization using bacterial enzyme–induced calcite precipitation (BEICP)

et al. 2019

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This paper examines the bio-derived stabilization of sand-only or sand-plus-silt soils using an extracted bacterial enzyme application to achieve induced calcite precipitation (ICP). As compared to conventional microbial induced calcite precipitation (MICP) methods, which use intact bacterial cells, this strategy that uses free urease catalysts to secure bacterial enzyme–induced calcite precipitation (BEICP) appears to offer an improved means of bio-stabilizing silty-sand soils as compared to that of MICP processing. Several benefits may possibly be achieved with this BEICP approach, including bio-safety, environmental, and geotechnical improvements. Notably, the BEICP bio-stabilization results presented in this paper demonstrate (i) higher rates of catalytic urease activity, (ii) a wider range of application with sand-plus-silt soil applications bearing low-plasticity properties, and (iii) the ability to retain higher levels of soil permeability after BEICP processing. Comparative BEICP versus MICP results for sand-only systems are presented, along with BEICP-based results for stabilized soil mixtures at 90:10 and 80:20 percentile sand:silt ratios. This BEICP method’s ability to obtain unconfined compressive strength results in excess of 1000 kPa with sand-plus-silt soil mixtures is particularly noteworthy.

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“…[16,18]). In the past 10 years, the development and application of this technique has greatly advanced [2,6,8,14,15,18,32]. Compared with conventional techniques based on chemical reactions, biogrouting exhibits a delayed effect; it solidifies after the grout solution has dispersed on the ground, allowing for materials such as fertilizer components to be used as solidifiers.…”

Section: Introductionmentioning

confidence: 99%

Biogeochemical simulation of microbially induced calcite precipitation with Pararhodobacter sp. strain SO1

Akiyama¹,

Kawasaki

2019

Acta Geotech.

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Biogrouting is a ground improvement technique, which utilizes microorganisms. The numerical simulation of biogrouting is important to ensure efficient operation and to assess the applicability to the target ground. In this study, we compared syringe-scale biogrouting with biogeochemical simulation. Parameters suitable for practical applications were included. The rate constant and half-saturation constant of the reaction rate law in ureolytic bacteria Pararhodobacter sp. strain SO1, obtained from the simulation based on the urease activity test, were 1 × 10-8 mol/mg/s and 0.635 M, respectively. To achieve the same mineral precipitation in measurement and simulation, a setting in which only the calcite precipitated was used. In the sequential simulation of the solidification test, a variation in discharged Ca 2+ concentration was reproduced by introducing an "adjustment index," which considers the microbial biomass contributing to the reaction. Moreover, for the re-injection test, in which microbes were injected again to further improve the biogrout strength, the settings were validated by the sequential simulation followed by predictive simulation on different injection dates. The results indicate that by conducting a biogeochemical simulation of calcite precipitation for biogrouting using ureolytic bacteria, the strength of biogrout can be predicted and managed.

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The Influence of Injection Conditions and Soil Types on Soil Improvement by Microbial Functions

Cited by 15 publications

References 2 publications

Review of the use of microorganisms in geotechnical engineering applications

Review of the use of microorganisms in geotechnical engineering applications

Sand and silty-sand soil stabilization using bacterial enzyme–induced calcite precipitation (BEICP)

Biogeochemical simulation of microbially induced calcite precipitation with Pararhodobacter sp. strain SO1

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