The influence of iron ions on ATP-hydrolases activity of cell membranes of rat colon smooth muscle and kidney

Aa, Kaplia

doi:10.15407/ubj87.01.083

Cited by 4 publications

(19 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CSM Na + ,K + -АТРase biochemical properties revealed in the own researches correspond to the specific features of the enzyme from different sources [12,18,19]. Thus, the data also represent the approach of the simple and reliable determination of the activity of Na + ,K + -АТРase in nonpurified CSM membranes, present in membrane-bound form with native microenvironment, necessary for functional congruence.…”

Section: T a B L E 2 Inhibition Parameters For Atp-hydrolases (і 50 supporting

confidence: 58%

“…Taken together [12,19], the data reflect the differences of the structural arrangement in the membrane for two ATP-hydrolases, important for the functional manifestation. The CSM Na + ,K + -АТРase biochemical properties revealed in the own researches correspond to the specific features of the enzyme from different sources [12,18,19].…”

Section: T a B L E 2 Inhibition Parameters For Atp-hydrolases (і 50 mentioning

confidence: 62%

“…In the previous researches for the first time we estimated functionally adequate Na + ,K + -ATPase activity in postmitochondrial cellular membrane fraction of the rat CSM by digitonin pretreatment [12,18,19]. This study is an application of the Ds-Na pretreatment procedure used for the first time for the CSM membranes.…”

Section: Resultsmentioning

confidence: 99%

“…This indicates the necessity for compliance with mild pretreatment procedure at room temperature, proper detergent/ protein ratio and considerable detergent dilution in ATPase medium far below CMC Summarizing in general, it is important to emphasize that the basic principles of the methodological procedure are determined by standard characteris tics and precaution of commonly used detergents in membranological studies [15,16,20,21] requiring mild pretreatment procedure at room temperature before incubation of permeabilized membranes with effectors or in ATPase medium at 37 °C. Thus, the methodological importance of the separation of the procedures of the detergent action, modification and/or ATPase reaction to minimize the deleterious unfolding detergent effect in the course of Na + ,K + -ATPase activity determination, including CSM preparations, is highlighted [12,[17][18][19]. It also diminishes the possible combined effect of detergents and membrane-active agents, enhancing enzyme unfolding and inactivation [17].…”

Section: Resultsmentioning

confidence: 99%

“…Aliquots 5-10 µl immedia tely were added into ATPase reaction mixture (0.5 ml) at 37 °С with dilution 50-100 times [18,19].…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Different sensitivity of Na(+),K(+)-ATPase and Mg(2+)-АТРase to ethanol and arachidonic acid in rat colon smooth muscle under pretreatment of cellular membranes with Ds-Na

Kaplia¹

2017

Ukr.Biochem.J.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: T a B L E 2 Inhibition Parameters For Atp-hydrolases (і 50 supporting

confidence: 58%

Section: T a B L E 2 Inhibition Parameters For Atp-hydrolases (і 50 mentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Aliquots 5-10 µl immedia tely were added into ATPase reaction mixture (0.5 ml) at 37 °С with dilution 50-100 times [18,19].…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Different sensitivity of Na(+),K(+)-ATPase and Mg(2+)-АТРase to ethanol and arachidonic acid in rat colon smooth muscle under pretreatment of cellular membranes with Ds-Na

Kaplia¹

2017

Ukr.Biochem.J.

Self Cite

View full text Add to dashboard Cite

show abstract

The influence of heavy metal ions, spermine and sodium nitroprusside on ATP-hydrolases of cell membranes of rat colon smooth muscle

Kaplia¹

2016

Ukr.Biochem.J.

Self Cite

View full text Add to dashboard Cite

The specific features of functional lability of the rat colon smooth muscle (CSM) АТР-hydrolases were studied. Na+,K+-AТРase activity is effectively inhibited by divalent ions of both transition (≥ 0,1 µM) and nontransition (≥ 1 µM) heavy metals in succession by efficiency: Cu2+ > Fe2+ ≥ Cd2+ (10 µM). Polyamine spermine (0,5-1,0 mM) is a weak Na+,K+-AТРase inhibitor at saturation concentrations of ions and substrate. Sodium nitroprusside (1 mM) as nitric oxide-generating compound exhibits weak Na+,K+-AТРase inhibition only after prolonged preincubation with membranes. Mg2+-АТР-hydrolase activity in all cases is much more resistant to studied agents. Considering the example of the CSM Na+,K+-AТРase it is assumed that enzyme has specific biochemical features that contribute to its role as a potential target and redox-sensor, mediating the pathological mechanisms of heavy metal intoxication and cell oxidative damage.

show abstract

Iron(IV) hexahydrazide clathrochelate complexes: the chronic toxicity study

Dukhnytskyi¹,

Kalachniuk²,

Derkach³

et al. 2020

Ukr J Ecol

View full text Add to dashboard Cite

One of the main tasks in the fight against anemia is to carry out preventive measures, to develop and to introduce new preparations containing iron, to provide the pharmaceutical market with anti-anemic agents. That's why to investigate the chronic toxic effect of Iron(IV) hexahydrazide clathrochelate (new iron-containing drug) in mice was the article's aim. The study of chronic toxic effect of Iron(IV) clathrochelate was performed on 45 white mice (19–25 g) separated into 3 groups: control (I) and two experimental (II and III). Animals of II and III groups were daily fed the water solution of Iron(IV) clathrochelate at a rate of 125.8 and 251.6 mg/kg of body weight, respectively. It was monitored the dynamics of body weight of white mice, relative coefficients of the mass of internal organs, hemoglobin content and morphological parameters of blood, biochemical indices of blood serum on the 10th, 20th and 30th day of the experiment. It was established a decrease of body weight by 16–21% and relative weight indices of internal organs (liver, kidney, heart, and spleen) – by 5– 43% in white mice of II and III experimental groups, respectively. Changes in the morphological composition of the blood showed stimulation of leukocytopoiesis. A significant decrease in creatinine (hypocreatinemia) and uric acid (hypouricemia) was observed, which indicates an increase in the filtration capacity of the renal glomeruli. Complex studies of the effect of Iron(IV) clathrochelate solution (at a dose of 125.8 and 251.6 mg/kg of body weight) did not cause any visible signs of intoxication and death of animals at the first time. Iron(IV) clathrochelate solution (at a dose of 125.8 mg/kg of body weight) is demonstrated more safe effect.

show abstract

The influence of iron ions on ATP-hydrolases activity of cell membranes of rat colon smooth muscle and kidney

Cited by 4 publications

References 16 publications

Different sensitivity of Na(+),K(+)-ATPase and Mg(2+)-АТРase to ethanol and arachidonic acid in rat colon smooth muscle under pretreatment of cellular membranes with Ds-Na

Different sensitivity of Na(+),K(+)-ATPase and Mg(2+)-АТРase to ethanol and arachidonic acid in rat colon smooth muscle under pretreatment of cellular membranes with Ds-Na

The influence of heavy metal ions, spermine and sodium nitroprusside on ATP-hydrolases of cell membranes of rat colon smooth muscle

Iron(IV) hexahydrazide clathrochelate complexes: the chronic toxicity study

Contact Info

Product

Resources

About