The influence of leaf age on methylxanthines, total phenolic content, and free radical scavenging capacity of Ilex paraguariensis aqueous extracts

Abstract

Please cite this article in press as: Blum-Silva, C.H., et al. The influence of leaf age on methylxanthines, total phenolic content, and free radical scavenging capacity of Ilex paraguariensis aqueous extracts. Revista Brasileira de Farmacognosia (2015), http://dx.BJP 84 1-6 Revista Brasileira de Farmacognosia xxx (2015) xxx-xxx w w w . s b f g n o s i a . o r g . b r / r e v i s t a Original Article a b s t r a c tYerba-mate (Ilex paraguariensis A. St. Hil., Aquifoliaceae) is a South American native species that is widely used for its industrial potential in the preparation of drinks, teas and cosmetics. Its properties are directly related to the presence of its chemical constituents, such as saponins, methylxanthines and phenolic compounds. This study aimed to investigate the influence of leaf age on methylxanthine and total phenolic contents by High Performance Liquid Chromatography and UV, as well as on free radical scavenging Q2 capacity by UV, of aqueous extracts of I. paraguariensis leaves. The results showed great variability in all the metabolites measured. Leaf ageing significantly increased the methylxanthine content and total phenolic content of the extracts. Free radical scavenging capacity was also significantly affected (p < 0.05) by leaf age. A positive correlation was observed, between the antioxidant activity and total phenolic content. Q3

Phytochemicals and bioactive properties of Ilex paraguariensis: An in-vitro comparative study between the whole plant, leaves and stems

“…Several authors evaluated the antioxidant activity of I. paraguariensis extracts (de Mejía et al, 2010;Peres et al, 2013;Blum-Silva et al, 2015), though they have used different methods or expressed their results in distinct units, making it impossible to compare them with our results. Berté et al (2011) reported a much lower DPPH scavenging activity for an aqueous spray-dried extract of yerba mate from Brazil (EC 50 value 2520 µg/mL) than the one determined herein.…”

“…Furthermore, the antioxidant (de Mejía, Song, Heck, & Ramirez-Mares, 2010;Peres, Tonin, Tavares & Rodriguez-Amaya, 2013;Blum-Silva, Chaves, Schenkel, Coelho & Reginatto, 2015;Boaventura et al, 2015b) and anti-inflammatory (Tsai, Tsai, Wuc, Tseng & Tsai, 2010;Arçari et al, 2011, Lanzetti et al, 2012 properties of aqueous yerba mate extracts have been extensively investigated. However, studies on the cytotoxic potential of I.…”

Early selection and classification of yerba mate progênies

“…These programs aim at selecting genotypes with additional adaptive traits to environmental cultivation conditions, high-leaf mass production, and tolerance to biotic and abiotic factors. Recently, emphasis has been placed on the inclusion traits linked to the content of some relevant chemical compounds that can directly affect the quality of commercial products and on their biological effects (Morais et al, 2009;González de Mejía et al, 2010;Sturion & Resende, 2010;Anesini et al, 2012;Barbosa et al, 2015;Blum-Silva et al, 2015;González Arbeláez et al, 2016;Cardozo Junior & Morand, 2016). However, increasing productivity is always desirable for breeding programs.…”

Novel Beverages of Yerba-Mate and Soy: Bioactive Compounds and Functional Properties

“…According to Dartora et al [27], yerba-mate directly exposed to sunlight has higher levels of phenolic compounds as compared with the shaded plant. Blum-Silva et al [28] reported that the concentration of phenolic compounds is higher in younger leaves. However, Strassmann et al [29] found greater concentration of total phenolic compounds in aqueous extracts of cultivated yerba-mate leaves, either young or adult (149.68 and 135.40 µg/mL, respectively) in comparison to native leaves (64.08 µg/mL and 59.30 µg/mL, respectively).…”