When growing high-quality grape seedlings, an effective organization of production is required, aimed at the intensification and development of the industry. Rooting grapes with lignified cuttings is one of the most effective and economical methods of reproduction. The article presents the results of three-year studies (2019-2021) to study the effect of biologically active substances on the rootability of lignified grape cuttings. The object of the study is the Victoria grape variety obtained by crossing Vitis amurensis and Vitis vinifera with the Save Vilar 12-304 variety in VNIIVIV im. Yakov Ivanovich Potapenko. The variety under study is a table variety with an early ripening period, characterized by increased resistance to powdery mildew, oidium and gray rot. Withstands low negative temperatures up to -26 ... - 27ºC, with a period from budding to removable ripeness of 115-120 days. During the experiment, records were made about the growth of callus, rooting and output of standard seedlings. When studying the effect of biologically active substances on lignified cuttings, they were treated with salicylic and β-indolyl butyric acids at concentrations of 2000 and 3000 mg/l. As a result of the conducted studies, it was found that the highest percentage of rooting was obtained in the variant using salicylic acid at a concentration of 2000 mg/l and reached 84.5% on average over three years. The same version of the experiment showed the maximum yield of standard seedlings - up to 76.3% on average for three years.