The influence of lododeoxyuridine pretreatment of various cell cultures on adenovirus multiplication

Abstract

Treatment of cell cultures with iododeoxyuridine (IUdR) before virus inoculation may enhance the subsequent virus multiplication. This effect was studied in seven kinds of cell cultures with seven human adenoviruses from six subgenera. IUdR (50 micrograms/ml) was added 24 h after the seeding of cells, left for 2 days, and removed before virus inoculation. IUdR had no effect on cell proliferation. Viral cytopathic effect was enhanced in many instances by IUdR pretreatment. However, virus multiplication was enhanced weakly in only four cases [adenovirus 7 in Vero, African green monkey kidney, cynomolgus monkey kidney; adenovirus 4 (Ad4) in HeLa cells], and strongly only for Ad4 in Vero cells, which are semipermissive for Ad4. IUdR pretreatment of Vero cells shortened the replication cycle for Ad4 and increased sensitivity for minimal virus concentrations about 1000-fold. From immunofluorescence experiments it appears that more than one infectious particle is required to infect an untreated Vero cell.

Evaluation of mixed cell types and 5-iodo-2'-deoxyuridine treatment upon plaque assay titers of human enteric viruses

“…A variety of additives have been evaluated for use in cell culture media in efforts to broaden the natural susceptibilities of cell lines to different virus types. These additives include, but are not limited to, chymotrypsin (10,17), DEAE dextran (5), erythrocyte extract (16), 5-iodo-2'-deoxyuridine (IDU; 1,6,8,9,15,18), and MgCl2 (19). Halogenated pyrimidine IDU has frequently been found to work well for this purpose, particularly in the case of adenoviruses.…”