The influence of organic solvents on phenylethylamines in capillary zone electrophoresis

Bolkvadze, Vyacheslav; Bondar, Denys; Vaher, Merike; Halling, Evelin; Gorbatšova, Jelena; Mazina-Šinkar, Jekaterina

doi:10.1016/j.chroma.2022.463169

Cited by 2 publications

(1 citation statement)

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“…The addition of appropriate organic reagents to the buffer solution has been demonstrated to increase the viscosity of the buffer and decrease Joule heat produced during electrophoresis, enhancing the separation efficiency of analytes. 34,35 Therefore, the influence of the buffer solution without organic reagents and with methanol, ACN, or isopropanol was investigated to assess the separation of analytes.…”

Section: Type and Proportion Of Organic Modifiersmentioning

confidence: 99%

Combination of large‐volume sample stacking with polarity switching and micelle electrokinetic chromatography for the analysis of anion compounds in Yangxinshi tablets

Liu,

Zhou,

et al. 2024

Phytochemical Analysis

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IntroductionYangxinshi tablet (YXST) is a traditional Chinese medicine preparation characterized by its high efficacy and safety for the treatment of cardiovascular diseases. Anionic compounds have been revealed as potential active components. However, there is currently limited research regarding its quality control.ObjectiveWe aimed to establish a strategy for the simultaneous separation and determination of five key anionic compounds in YXST.MethodA sensitive and efficient analytical method was developed and applied for the simultaneous separation and determination of five key compounds in YXST using large‐volume sample stacking with polarity switching and micelle electrokinetic chromatography (LVSS‐PS‐MEKC) coupled with diode array detection. Crucial parameters, including sample volume, applied voltage, composition and pH of the running buffer, concentration of organic modifier, and switching time of the polarity, were systematically evaluated and optimized using a single variable method to enhance separation performance. Furthermore, the impact of cyclodextrin and sodium dodecyl sulfate as electrolyte modifiers was also investigated.ResultsUnder the optimal conditions, baseline separation of the five compounds (daidzein, puerarin, glycyrrhiztinic acid, chlorogenic acid, and salvianolic acid B) was achieved within 20 min. In comparison to the conventional MEKC mode, the constructed LVSS‐PS‐MEKC method exhibited a more than sixfold increase in the enrichment factor. The method was validated in terms of linearity, precision, accuracy, 24 h stability, and recovery and successfully applied to analyze YXST samples.ConclusionA sensitive strategy was developed for the simultaneous separation and determination of five key anionic components in YXST, offering a robust and efficient strategy for pharmaceutical analysis.

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Section: Type and Proportion Of Organic Modifiersmentioning

confidence: 99%