The influence of physical state on the absorption and fluorescence spectra of organic substances

A study of the adsorption of dyes on bovine serum albumin by the method of polarization of fluorescence

“…Glycerol, concentrated sugar solution and ethanol gave smaller shifts to the red with respect to water, although the refractive indices and hence the volume-polarizabilities of these liquids was as high or higher than the benzene and 'essence'. A similar view is admitted by Seshan (1936). It may be concluded that the behaviour of the fluorescent dyes when adsorbed on to serum albumin identifies the site of adsorption with material of low dielectric constant.…”