The Influence of Piperine on the Radioprotective Effect of Curcumin in Irradiated Human Lymphocytes

Abstract

İyonize radyasyon (IR) normal hücrelerde DNA hasarına neden olarak genotoksisiteye neden olur. Curcumin ve piperin ile ortak tedavinin insan normal lenfositlerinde iyonlaştırıcı radyasyonun neden olduğu genotoksisiteye karşı koruyucu etkileri araştırıldı. Gereç ve Yöntemler: İnsan kan numuneleri, farklı konsantrasyonlarda (5, 10 ve 25 µg/mL) kurkumin ve/veya (2.5 µg/mL) piperin içerisinde ile ön muamele edildi ve daha sonra 1.5 Gy'lik bir dozda IR'ye maruz bırakıldı. Curcumin ve piperinin radyo-koruyucu etkileri mikronükleus (MN) testi ile değerlendirildi. Bulgular: Kurkumin ve piperin, lenfositlerde IR tarafından indüklenen MN yüzdesini azaltmıştır. Sadece piperin, tüm konsantrasyonlarda tek başına uygulanan kurkumin ile karşılaştırıldığında, IR tarafından indüklenen genotoksisiteyi önemli ölçüde azaltmıştır. Düşük konsantrasyonda 5 µg/mL'de piperin ve kurkumin kombinasyonu ile ilave bir radyo-koruyucu etki gözlenirken, bu sinerjistik etki 10 ila 25 µg/mL'lik yüksek konsantrasyonlarda kurkuminle gözlenmedi. Sonuç: Piperin, kurkuminle karşılaştırıldığında düşük konsantrasyonda güçlü bir radyo-koruyucu etkiye sahiptir. Bununla birlikte, düşük konsantrasyonda piperin ve kurkumin ile birlikte yapılan muamele ile ilave bir radyo-koruyucu etki gözlenirken, piperin yüksek konsantrasyonda kurkumin ile birlikte muamele edildiğinde normal lenfositlerde MN oranını artırmıştır. Anahtar kelimeler: Kurkumin, piperin, radyo-koruyucu genotoksisite, iyonize radyasyon ÖZ Objectives: Ionizing radiation (IR) induces DNA damage in normal cells, leading to genotoxicity. The radioprotective effects of co-treatment with curcumin and piperine were investigated against genotoxicity induced by IR in human normal lymphocytes. Materials and Methods: Human blood samples were pretreated with curcumin at different concentrations (5, 10, and 25 µg/mL) and/or piperine (2.5 µg/mL) and then were exposed to IR at a dose 1.5 Gy. The radioprotective effects of curcumin and piperine were assessed by micronucleus (MN) assay. Results: Curcumin and piperine reduced the percentage of MN induced by IR in lymphocytes. Piperine alone significantly reduced genotoxicity induced by IR as compared to curcumin alone at all concentrations. An additive radioprotective effect was observed with combination of piperine and curcumin at the low concentration of 5 µg/mL, while this synergistic effect was not observed with curcumin at the higher concentrations of 10 and 25 µg/mL. Conclusion: Piperine has a potent radioprotective effect at low concentration as compare to curcumin. However, an additive radioprotective effect was observed with co-treatment with piperine and curcumin at low concentration, while piperine increased the percentage of MN in normal lymphocytes when co-treated with curcumin at higher concentration.

Piperine – An Immunomodulator and Inflammation Mitigator

“…It has been reported that piperine exerts a significant radioprotection in irradiated human lymphocytes expressed by a marked diminution of micronuclei number compared with irradiated non piperine-treated cells. This effect was more impressive when the lymphocytes were pretreated with both piperine and curcumin [51]. Rats given piperine before irradiation showed lesser elevation in serum TNF-α, IL-1β and IL-6 [52].…”

Nanodrugs with intrinsic radioprotective exertion: Turning the double‐edged sword into a single‐edged knife

“…Accumulative studies have shown that CUR is imparted with radioprotective functions on the hematopoietic system, skin, lungs, hearts, eyes, brain tissue, and other organs. [63][64][65][66][67][68][69][70] Impressively, an NIR-triggered TPGS-BCNPs has been recently constructed to load and deliver curcumin to enhance its bioavailability, which has been well proven to exhibit enhanced radioprotective capability for normal tissues. In addition, the excellent performance of RSV on radioprotection inspired the scientists as well.…”

Connection between Radiation-Regulating Functions of Natural Products and miRNAs Targeting Radiomodulation and Exosome Biogenesis

“…Zingerone [214] miR-200c-3p [215] Piperine [216] miR-181c-3p [217] Berberine [9] miR-182-5p [218] miR-17-5p [219], miR-383-3p, miR-383-5p [220] ↓…”

“…Lycopene inhibited atrazine-induced ROS generation and apoptosis in the B-cell zone by downregulating miR-27a-3p [213] (Table 1). Piperine (2.5 µg/mL), a black-pepper-derived alkaloid, reduced DNA damage in 1.5 Gy irradiated lymphocytes [216]. Piperine suppressed leptin-induced anti-breastcancer effects, inhibiting proliferation and migration, and inhibited breast tumor growth in obese mice by upregulating miR-181c-3p [217].…”