The influence of poly(ethylene glycol) on the molecular dynamics within the glycocalyx

Pratsch, Lothar; Herrmann, Andreas; Schwede, Ilona; Meyer, H.

doi:10.1016/0005-2736(89)90393-3

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…Thus compared with PLys, PEI appears to promote the accessibility of GTlb to cell surface probes. The influence of PEI on GTlb labelling may be mediated through the alteration of the physico chemical properties of water surrounding the cells as is the case for polyethyleneglycol [44], This phenomenon may also account for the faster attachment of neuronal cells on PEI followed by a higher cell growth compared with PLys cells (to be published).…”

Section: Modulation O F Radioiodinated Lipidmentioning

confidence: 99%

Expression of Plasma Membrane and Cell Surface Phospholipids and Gangliosides of Chick Embryo Neurons Grown in Primary Cultures: Developmental Studies

Lelong

Frechard²,

Crémel³

et al. 1991

Dev Neurosci

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Plasma membranes isolated from chick embryo neurons after 2 days (division phase) and 5 days (maturation phase) in culture showed no differences in the phospholipid (PL) pattern or in the plasma membrane average fluidity. Significant ganglioside (Ggl) variations were only found in GM1 and GD3 which represent 35% of the total plasma membrane N-acetylneuraminic acid (NeuNac) content (1.8-fold GM1 NeuNac increase, 1.4-fold GD3 NeuNac reduction). Lactoperoxidase-catalyzed radioiodination (¹²⁵I) under saturating conditions indicated that the hydrophobic core of cell surface Ggl was more exposed to the hydrophilic extracellular environment than that of PL. Nevertheless, when interneuronal contacts start to form, the ¹²⁵I-labelling of both PL and Ggl dropped progressively throughout the maturation phase. Further analysis revealed that (1) plasma membrane GD3 was not accessible to the enzymatic labelling, and (2) surface phosphatidylethanolamine (PE) and GT1b became more accessible to the external medium during development, whereas the opposite was found for phosphatidylcholine (PC) and GM1. The overall profile of radioiodinated PL and Ggl was unaltered when poly-L-lysine was substituted for polyethyleneimine as culture substratum, except for an increase in GT1b labelling 24 h after plating. These results suggest the existense of a ''buffering'' mechanism protecting the neuronal plasma membrane during cellular growth from marked changes in fluidity, polar lipid composition and relative localization of polar lipids with regard to the extracellular environment. In addition, our findings suggest that: (1) GD3, which is at maximal levels during the division phase, is not exposed to the extracellular environment; (2) PE, PC, GM1 and GT1b undergo subtle topological reorganization as cellular maturation proceeds, and (3) the culture substratum may modulate the exposure of certain cell surface glycolipid components.

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Section: Modulation O F Radioiodinated Lipidmentioning

confidence: 99%

Expression of Plasma Membrane and Cell Surface Phospholipids and Gangliosides of Chick Embryo Neurons Grown in Primary Cultures: Developmental Studies

Lelong

Frechard²,

Crémel³

et al. 1991

Dev Neurosci

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show abstract

“…Although the influence of PEG on the fusion of cells is not clear, a direct interaction with membrane constituents has been ruled out. Apparently, PEG alters the physicochemical properties of the water surrounding the cells, thereby creating a depletion layer between the two opposing membranes (13). PEG a t such low concentrations used in our experiments, however, does not possess any fusing activity per se.…”

Section: Discussionmentioning

confidence: 99%