The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation

Abstract

The objective is to investigate the influence of PM2.5 exposure on peripheral blood lymphocyte subsets in pregnant mice and the antagonism of quercetin on adverse effects induced by PM2.5 exposure. Pregnant mice were randomly divided into control group, PM2.5 model group and 3 quercetin intervention groups. Dams in all groups except the control group were exposed to PM2.5 suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Meanwhile, each dam was given 0.15% carboxymethylcellulose sodium (CMCS) (control group & PM2.5 model group) and different doses of quercetin (quercetin intervention groups) by gavage once a day from GD0 to GD17. The percentage of lymphocyte subsets, Biomarkers of systemic inflammation injuries (IL-2, IL-6, IL-8 & TNF-α) and oxidative stress indicators (CAT, GSH & HO-1) in peripheral blood of the dams were analyzed. The number of T cells increased, accompanied by increased level of IL-2, IL-6, IL-8 and HO-1 due to PM2.5 exposure. Less CD4+ and CD8+ T cells were counted in 100 mg/kg quercetin intervention group, compared with PM2.5 model group. Quercetin may inhibit cytokine production, especially in IL-6 and IL-8 and may upgrade the level of HO-1. Our findings indicate that PM2.5 could significantly influence the distribution of T-lymphocyte subsets, activate inflammatory reaction and elevate oxidative stress level in peripheral blood of pregnant mice. Certain dose of quercetin administration during pregnancy may protect the dams against the adverse effects through various ways.

Chemical screen identifies a geroprotective role of quercetin in premature aging

“…To further determine the molecular mechanisms of Que, we performed RNA-seq analysis and revealed that the upregulated genes in Que-treated WS hMSCs were primarily enriched in cell cycle, nuclear division and chromosome segregation pathways, consistent with the cellular phenotypes observed. Previous studies on Que have mainly focused on its anti-carcinogenic (Ansgar, 2013; Darband Saber et al, 2018), anti-inflammatory (Griffiths et al, 2016; Li et al, 2016a; Liu et al, 2017; Salvatore, 2010), anti-viral (Chiow et al, 2016; Li et al, 2016a), as well as anti-oxidant effects (Sohn et al, 2018). However, the mechanisms of Que against human stem cell aging remain to be elucidated.…”

Quercetin Intervention Alleviates Offspring’s Oxidative Stress, Inflammation, and Tight Junction Damage in the Colon Induced by Maternal Fine Particulate Matter (PM2.5) Exposure through the Reduction of Bacteroides

“…The dosages of PM 2.5 and quercetin were determined in our previous research [ 23 , 24 , 25 ]. The PM 2.5 sample was prepared by several processes: collecting, agitating, filtering, and lyophilizing.…”

“…The PM 2.5 sample was prepared by several processes: collecting, agitating, filtering, and lyophilizing. A detailed description can be seen in our previous articles [ 23 , 24 ]. Phosphate-buffered saline was used as the solvent to dissolve the PM 2.5 powder.…”

Inhale, exhale: Why particulate matter exposure in animal models are so acute? Data and facts behind the history

“… [69] Intratracheal instillation The objective is to investigate the influence of PM 2.5 exposure on peripheral blood lymphocyte subsets in pregnant mice and the antagonism of quercetin on adverse effects induced by PM 2.5 exposure. [70] Intratracheal instillation We intend to investigate the toxic effects of PM 2.5 during summer and winter on reproductive cells and tissues and focus on endoplasmic reticulum stress (ERS) to illustrate the possible molecular mechanisms. [71] Intratracheal instillation We wished to investigate the impact of PM 2.5 on placenta and prenatal outcomes and its related mechanisms in a rat model.…”