The influence of roughness on stem cell differentiation using 3D printed polylactic acid scaffolds

Feng, Kuan‐Che; Pinkas-Sarafova, Adriana; Ricotta, Vincent; Cuiffo, Michael; Zhang, Linxi; Guo, Yichen; Chang, Chung-Chueh; Halada, Gary P.; Simon, Marcia; Rafailovich, Miriam

doi:10.1039/c8sm01797b

Cited by 17 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10,35 Feng et al suggested that such an uneven surface is a result of sharkskin surface instability. 43 Sharkskinlike surface irregularity has been reported in previous literature related to polymer extrusion. 32,43 Sharkskin surface on the extruded polymer is observed due to stress singularity developed at the nozzle exit, resulting in rapid tensile deformation of polymer molecules in the extrudate.…”

Section: Discussionmentioning

confidence: 80%

“…43 Sharkskinlike surface irregularity has been reported in previous literature related to polymer extrusion. 32,43 Sharkskin surface on the extruded polymer is observed due to stress singularity developed at the nozzle exit, resulting in rapid tensile deformation of polymer molecules in the extrudate. 32 As shown in Figure S5, the printed polymer fibers preserve the sharkskin patterns developed during extrusion, greatly reducing the optical transparency of the top surface.…”

Section: Discussionmentioning

confidence: 80%

See 1 more Smart Citation

Facile Route for 3D Printing of Transparent PETg-Based Hybrid Biomicrofluidic Devices Promoting Cell Adhesion

Mehta

Sudhakaran

Rath

2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

3D printing has emerged as a promising fabrication technique for microfluidic devices, overcoming some of the challenges associated with conventional soft lithography. Filament-based polymer extrusion (popularly known as fused deposition modeling (FDM)) is one of the most accessible 3D printing techniques available, offering a wide range of low-cost thermoplastic polymer materials for microfluidic device fabrication. However, low optical transparency is one of the significant limitations of extrusion-based microfluidic devices, rendering them unsuitable for cell culture-related biological applications. Moreover, previously reported extrusion-based devices were largely dependent on fluorescent dyes for cell imaging because of their poor transparency. First, we aim to improve the optical transparency of FDM-based microfluidic devices to enable bright-field microscopy of cells. This is achieved using (1) transparent polymer filament materials such as poly(ethylene terephthalate) glycol (PETg), (2) optimized 3D printing process parameters, and (3) a hybrid approach by integrating 3D printed microfluidic devices with cast poly(dimethylsiloxane) (PDMS) blocks. We begin by optimizing four essential 3D printing process parameters (layer height, printing speed, cooling fan speed, and extrusion flow), affecting the overall transparency of 3D printed devices. Optimized parameters produce exceptional optical transparency close to 80% in 3D printed PETg devices. Next, we demonstrate the potential of FDM-based 3D printing to fabricate transparent micromixing devices with complex planar and nonplanar channel networks. Most importantly, cells cultured on native 3D printed PETg surfaces show excellent cell attachment, spreading, and proliferation during 3 days of culture without extracellular matrix coating or surface treatment. Next, we introduce L929 cells inside hybrid PETg-PDMS biomicrofluidic devices as a proof of concept. We demonstrate that 3D printed hybrid biomicrofluidic devices promote cell adhesion, allow bright-field microscopy, and maintain high cell viability for 3 days. Finally, we demonstrate the applicability of the proposed fabrication approach for developing 3D printed microfluidic devices from other FDM-compatible transparent polymers such as polylactic acid (PLA) and poly(methyl methacrylate) (PMMA).

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 80%

Facile Route for 3D Printing of Transparent PETg-Based Hybrid Biomicrofluidic Devices Promoting Cell Adhesion

Mehta

Sudhakaran

Rath

2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…It is known that the morphological characteristics of scaffolds, such as pore size and gap width between struts, affect cell adhesion, proliferation and differentiation [ 190 ]. It is possible to control cell fate by modification of the structural properties of a scaffold [ 191 ]. Recently, 3D printing technology has been used to produce tailored PLA scaffolds for pulp tissue engineering.…”

Section: Synthetic Biomaterials Scaffoldsmentioning

confidence: 99%

Biomaterial scaffolds for clinical procedures in endodontic regeneration

Liu

Lü

Jiang

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

“…For example, in a recent study by Feng and colleagues, they compared two different techniques in fabricating a PLA scaffold, either molding via standard extrusion processes or 3D printed, and its influence on dental pulp cells. The results indicated that manufacturing techniques can influence differences in cell migration, morphology, and differentiation marker expression [72]. Another study by Hu and colleagues used 3D printed molds to create cellularized conduits for peripheral nerve regeneration, which showed comparable results to the use of autografts in repairing peripheral nerve defects (Figure 3) [73].…”

Section: Pre-clinical and Clinical Applicationsmentioning

confidence: 99%

The Applications of 3D Printing for Craniofacial Tissue Engineering

et al. 2019

View full text Add to dashboard Cite

Three-dimensional (3D) printing is an emerging technology in the field of dentistry. It uses a layer-by-layer manufacturing technique to create scaffolds that can be used for dental tissue engineering applications. While several 3D printing methodologies exist, such as selective laser sintering or fused deposition modeling, this paper will review the applications of 3D printing for craniofacial tissue engineering; in particular for the periodontal complex, dental pulp, alveolar bone, and cartilage. For the periodontal complex, a 3D printed scaffold was attempted to treat a periodontal defect; for dental pulp, hydrogels were created that can support an odontoblastic cell line; for bone and cartilage, a polycaprolactone scaffold with microspheres induced the formation of multiphase fibrocartilaginous tissues. While the current research highlights the development and potential of 3D printing, more research is required to fully understand this technology and for its incorporation into the dental field.

show abstract

The influence of roughness on stem cell differentiation using 3D printed polylactic acid scaffolds

Abstract: With the increase in popularity of 3D printing, an important question arises as to the equivalence between devices manufactured by standard methods vs. those presenting with identical bulk specifications, but manufactured via fused deposition modeling (FDM) printing.

Cited by 17 publications

References 26 publications

Facile Route for 3D Printing of Transparent PETg-Based Hybrid Biomicrofluidic Devices Promoting Cell Adhesion

Facile Route for 3D Printing of Transparent PETg-Based Hybrid Biomicrofluidic Devices Promoting Cell Adhesion

Biomaterial scaffolds for clinical procedures in endodontic regeneration

The Applications of 3D Printing for Craniofacial Tissue Engineering

Contact Info

Product

Resources

About