The influence of selection on reaction to stress in mice. III. Influence of fasting, immobilization and exposure to cold on lactate dehydrogenase and aldolase activity in the liver and kidney

Abstract

Summary The experiment was conducted on 6‐week‐old mice selected for a high rate of body‐weight gain. Model stressors were used: fasting for 24 and 48 h, immobilization for 12 and 48 h, and cooling, i.e. reducing outside temperature to +5°C, over 12 h. Measurements were made of the level of glucose in blood, the aldolase activity and the LDH activity in the liver and kidney, and the level of total protein. After 48 h of fasting, the weight of the body, liver and spleen decreased. Fasting and immobilization caused a decrease in the level of blood glucose and in the activity of aldolase and LDH, which was more marked in selected mice. The cold exposure increased the blood‐serum glucose level. The results presented suggest that mice selected for a high rate of body weight gain seem to be less resistant to unfavorable environmental factors — fasting, immobilization and cooling — than the unselected animals. Zusammenfassung Einflußder Selektion auf Stressreaktion bei Mäusen Nach 48‐stündigen Hungern kommt es bei Mäusen zu einer Abnahme des Körpergewichtes sowie des Leber‐und Milz gewichtes. Nach 48 Studen Hunger sowie bei Immobilisierung wurde einen Abfall des Blutglukosespiegels, weiter auch eine Abnahme der LDH‐und Aldolaseaktivität in Leber und Niere, insbesonderes bei den selektierten Tieren, beobachtet. Nach 12 stündige Kühlung bei +5°C kommt es zu einem Anstieg der Blutglukosespiegel. Die Ergebnisse zeigen, daßselektierte Tiere empfindlicher als Kontrolltiere gegen Belastungen sind.

The influence of selection on reaction to stress in mice V. The effect of starvation and glutathione injection on the activity of N‐acetyl‐beta‐glucosaminidase and the level of glutathione in the liver

“…This experiment continued the previous studies of R ysińska et al (1992) and K ołataj et al (1992, 1995, 1996).…”

The influence of selection on reaction to stress in mice.VII. Activity of some lysosomal enzymes in the muscle, spleen, kidney and liver

“…This experiment continued previous studies ( Witek 1998 ; Koątaj et al . 1992 , 1995, 1996; Rysińska et al .…”

“…This experiment continued previous studies ( Witek 1998 ; Koątaj et al . 1992 , 1995, 1996; Rysińska et al . 1992 ) and compared the activity of lysosomal enzymes in organs of mice selected for a lower feed consumption (per gram of body weight gain) and unselected control mice.…”

“…These experiment proved that the selected animals had higher activities of the lysosomal enzymes in the liver, kidney, muscle and spleen than the unselected, control ones, and that selection influenced these differences significantly. The earlier studies ( Ko&aogon,taj et al . 1995 , 1996; Witek & Ko&aogon,taj 1998) showed that selection for a high rate of body weight gain can change the biochemical reactivity of an organism in connection with damaging environmental factors.…”

The influence of selection on reaction to stress in mice. IX. Effect of dietary protein level on activity of lysosomal enzymes in liver and kidney

“…The studies of Witek and Kołątaj (1998) and Kołątaj et al (1998) indicate that the lysosomal system is significantly connected with homeostasis. Homeostatic disturbances caused by prolonged selection may also be observed in the activity of non‐lysosomal enzymes ( Kołątaj et al 1995, 1996). The lysosome is the major site of intracellular protein degradation.…”