The influence of storage time and temperature on propofol concentrations in canine blood and plasma

Cox, Sherry; Bailey, Joan; Okafor, Chika C.; Seddighi, Reza; Doherty, Thomas J

doi:10.7717/peerj.3476

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Since propofol is a short-acting intravenous anesthetic, it has the advantage of rapid induction and rapid resuscitation and is often used in a variety of animal experiments and surgical procedures [ 18 ]. As a new intravenous anesthetic, propofol may play a protective role in the body through the influence to cytokines produced by endotoxemia [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-α correlating with high-mobility group box 1 expression in lipopolysaccharides-stimulated RAW 264.7 cells

Jia

Sun

et al. 2017

BMC Anesthesiol

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BackgroundStudies have found that propofol can inhibit endotoxin-induced monocyte-macrophages to produce various inflammatory factors. This study is to disclose whether the propofol affects the expression of high-mobility group box 1 (HMGB1) in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells and the release of interleukin-6 (IL-6), 8 (IL-8) and tumor necrosis factor-α (TNF-α).MethodsRAW 264.7 cells were divided into four groups for intervention. After culturing for 16 h, the cells and culture supernatants were collected. The expression of HMGB1 in RAW 264.7 cells was detected by Western blot. The levels of IL-6, IL-8 and TNF-α in supernatants of cells were determined by enzyme-linked immunosorbent assay (ELISA).ResultsStimulation of LPS increased the expression of HMGB1 and promoted the release of IL-6, IL-8 and TNF-α in supernatants of RAW 264.7 cells (p < 0.05); however, propofol down-regulated the expression of LPS-stimulated HMGB1 and reduced the LPS-stimulated releases of IL-6, IL-8 and TNF-α in supernatants of RAW 264.7 cells (p < 0.05). Moreover, the releases of IL-6, IL-8 and TNF-α intimately correlated with the expression of HMGB1 in this process (p < 0.05).ConclusionPropofol inhibited the releases of IL-6, IL-8 and TNF-α in LPS-stimulated RAW 264.7 cells, and the levels of IL-6, IL-8 and TNF-α intimately correlated with the expression of HMGB1, which indicating that propofol may prevent inflammatory responses through reducing the releases of these cytokines and inflammatory mediators.

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Section: Discussionmentioning

confidence: 99%

Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-α correlating with high-mobility group box 1 expression in lipopolysaccharides-stimulated RAW 264.7 cells

Jia

Sun

et al. 2017

BMC Anesthesiol

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“…Propofol in whole blood was suggested to be stored at 4°C instead of −20°C because of significant propofol loss when frozen. 27 It was reported that propofol in blood was stable for 12 weeks at 4°C. 28 Final…”

Section: Methods Validationmentioning

confidence: 99%

A sensitive liquid chromatography method for analysis of propofol in small volumes of neonatal blood

Nicolaï

Smits

et al. 2019

J Clin Pharm Ther

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What is known and objective: Sampling volumes of blood from neonates is necessarily limited. However, most of the published propofol analysis assays require a relatively large blood sample volume (typically ≥0.5 mL). Therefore, the aim of the present study was to develop and validate a sensitive method requiring a smaller sample volume (0.2 mL) to fulfill clinically relevant research requirements. Methods: Following simple protein precipitation and centrifugation, the supernatant was injected into the HPLC-fluorescence system and separated with a reverse phase column. Propofol and the internal standard (thymol) were detected and quantified using fluorescence at excitation and emission wavelengths of 270 nm and 310 nm, respectively. The method was validated with reference to the Food and Drug Administration (FDA) guidance for industry. Accuracy (CV, %) and precision (RSD, %) were evaluated at three quality control concentration levels (0.05, 0.5 and 5 µg/mL). Results and discussion: Calibration curves were linear in the range of 0.005-20 µg/ mL. Intra-and interday accuracy (−4.4%-13.6%) and precision (0.2%-5.8%) for propofol were below 15%. The calculated LOD (limit of detection) and LLOQ (lower limit of quantification) were 0.0021 µg/mL and 0.0069 µg/mL, respectively. Propofol samples were stable for 4 months at −20°C after the sample preparation. This method was applied for analyzing blood samples from 41 neonates that received propofol, as part of a dose-finding study. The measured median (range) concentration was 0.14 (0.03-1.11) µg/mL, which was in the range of the calibration curve. The calculated median (range) propofol half-life of the gamma elimination phase was 10.4 (4.7-26.7) hours. What is new and conclusion: A minimal volume (0.2 mL) of blood from neonates is required for the determination of propofol with this method. The method can be used to support the quantification of propofol drug concentrations for pharmacokinetic studies in the neonatal population.

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Drug detection in decomposed cadavers confirms testimonial evidence in a case of serial homicides

Ottaviano

Tavone

Scipione

et al. 2021

Forensic Science International

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The influence of storage time and temperature on propofol concentrations in canine blood and plasma

Cited by 4 publications

References 18 publications

Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-α correlating with high-mobility group box 1 expression in lipopolysaccharides-stimulated RAW 264.7 cells

Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-α correlating with high-mobility group box 1 expression in lipopolysaccharides-stimulated RAW 264.7 cells

A sensitive liquid chromatography method for analysis of propofol in small volumes of neonatal blood

Drug detection in decomposed cadavers confirms testimonial evidence in a case of serial homicides

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