The Influence of Streptozotocin Diabetes and Metformin on Erythrocyte Volume and on the Membrane Potential and the Contractile Characteristics of the Extensor Digitorum Longus and Soleus Muscles in Rats

McGuire, Michelle K.; MacDermott, M

doi:10.1111/j.1469-445x.1999.01916.x

Cited by 28 publications

(26 citation statements)

References 13 publications

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“…1, Table 1). This result was consonant with previous studies showing that the STZ-induced diabetic rat endures a fast-twitch, fiber-specific atrophy of hindlimb muscles (5,14,39). Also, DIA induced a profound decrease of SDH activity compared with CONT (Fig.…”

Section: Effects Of Diabetes On Morphological Properties Of Spinotrapsupporting

confidence: 80%

In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes

Eshima

Tanaka

Sonobe

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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([Ca 2ϩ ]i) accumulation in Type 1 diabetes are unclear. We tested the hypothesis that, following repeated bouts of muscle contractions, the rise in resting [Ca 2ϩ ]i evident in healthy rats would be increased in diabetic rats and that these changes would be associated with a decreased cytoplasmic Ca 2ϩ -buffering capacity. Adult male Wistar rats were divided randomly into diabetic (DIA; streptozotocin, ip) and healthy control (CONT) groups. Four weeks later, animals were anesthetized and spinotrapezius muscle contractions (10 sets of 50 contractions) were elicited by electrical stimulation (100 Hz). Ca 2ϩ imaging was achieved using Fura-2 AM in the spinotrapezius muscle in vivo (i.e., circulation intact). The ratio (340/380 nm) was determined from fluorescence images following each set of contractions for estimation of [Ca 2ϩ ]i. Also, muscle Ca 2ϩ buffering was studied in individual myocytes microinjected with 2 mM Ca 2ϩ solution. After muscle contractions, resting [Ca 2ϩ ]i in DIA increased earlier and more rapidly than in CONT (P Ͻ 0.05 vs. precontraction). Peak [Ca 2ϩ ]i in response to the Ca 2ϩ injection was significantly higher in CONT (25.8 Ϯ 6.0% above baseline) than DIA (10.2 Ϯ 1.1% above baseline). Subsequently, CONT [Ca 2ϩ ]i decreased rapidly (Ͻ15 s) to plateau 9 -10% above baseline, whereas DIA remained elevated throughout the 60-s measurement window. No differences in SERCA1 and SERCA2 (Ca 2ϩ uptake) protein levels were evident between CONT and DIA, whereas ryanodine receptor (Ca 2ϩ release) protein level and mitochondrial oxidative enzyme activity (succinate dehydrogenase) were decreased in DIA (P Ͻ 0.05). ] i evident in healthy rats would be magnified in muscles of diabetic rats and further that these changes would be associated with attenuation of the Ca 2ϩ -handling system within the in vivo environment. METHODS AnimalsMale Wistar rats (n ϭ 43, 10 wk of age; Japan SLC, Shizuoka, Japan) were used in this study. Rats were maintained on a 12:12-h light-dark cycle and received food and water ad libitum. Rats were divided into two groups: healthy control (CONT) and diabetic (DIA) rats. Rats were anesthetized using isoflurane and given intraperitoneal injection of 45 mg/kg body wt of streptozotocin (STZ; S0130, SigmaAldrich St. Louis, MO) prepared fresh in saline solution. CONT animals were injected with the saline solution. Urine glucose levels of rats were measured (New Uriesu Ga, Terumo, Japan) 2 days after STZ injection with the onset of diabetes raising glucose concentrations above 500 mg/dl. These measurements of urine glucose were continued each week for 4 wk. At experiment completion, blood was collected from a tail vein puncture to confirm that the blood glucose level exceeded 300 mg/dl. All experiments were conducted under the guidelines established by the Physiological Society of Japan and were approved by University of Electro-Communications Institutional Animal Care and Use Committee. The rats were anesthetized using pentobarbital sodium (60 mg/kg ip), and supplemental dose...

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Section: Effects Of Diabetes On Morphological Properties Of Spinotrapsupporting

confidence: 80%

In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes

Eshima

Tanaka

Sonobe

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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“…The age chosen for induction of diabetes is consistent with most previous studies of this model (10,12,23,33,39,40,41,51,55,56). Animals were studied 4 wk after streptozotocin or buffer injection, as this amount of time postinjection is in the middle of the range used in previous studies (10,12,19,23,31,33,39,40,41,51,55,56,63). Furthermore, 4 wk is in line with the two previous diabetic heart gene expression array studies, which examined animals 2 wk post-streptozotocin (31) or 3 days, 4 wk, and 6 wk poststreptozotocin (19).…”

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 66%

Oxidoreductase, morphogenesis, extracellular matrix, and calcium ion-binding gene expression in streptozotocin-induced diabetic rat heart

Lunteren

Moyer

2007

American Journal of Physiology-Endocrinology and Metabolism

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“…Several investigations have been concerned with the effect of diabetes on rodent hindlimb skeletal muscles. They have shown that diabetes impairs muscle contractility and induces muscle atrophy [1][2][3][4] . Different hypotheses have been formulated to explain the alterations in muscle function in the diabetic state (see Discussion).…”

Section: Introductionmentioning

confidence: 99%

Diabetes Provides an Unfavorable Environment for Muscle Mass and Function after Muscle Injury in Mice

Vignaud¹,

Ramond²,

Hourdé³

et al. 2007

Pathobiology

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It is of common knowledge that diabetes decreases skeletal muscle contractility and induces atrophy. However, how hyperglycemia and insulin deficiency modify muscle mass and neuromuscular recovery after muscle injury is not well known. We have analyzed two models of diabetes: streptozotocin (STZ)-treated Swiss mice and Akita mice that spontaneously develop diabetes. A fast muscle, the tibialis anterior, was injured following injection of a myotoxic agent (cardiotoxin). Neuromuscular function was evaluated by examining in situ isometric contractile properties of regenerating muscles in response to nerve stimulation 14, 28 and 56 days after myotoxic injury. We found that STZ-induced diabetes reduces muscle weight and absolute maximal tetanic force in both regenerating and uninjured muscles (p = 0.0001). Moreover, it increases specific maximal tetanic force and tetanic fusion in regenerating and uninjured muscles (p = 0.04). In the Akita mice, diabetes decreases muscle weight and absolute maximal tetanic force, and increases tetanic fusion in both regenerating and uninjured muscles (p ≤ 0.003). Interestingly, STZ-induced diabetes exerts more marked effects than diabetes of genetic origin, in particular on muscle weight. This reduction in muscle mass was not due to an increased expression of the atrogenes MuRF1 and atrogin-1 during STZ-induced diabetes. The present study in mice demonstrates that both models of diabetes impair regenerating muscles as well as uninjured muscles. Regenerating fast muscles are weaker, lighter and slower in diabetic compared with nondiabetic mice.

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The Influence of Streptozotocin Diabetes and Metformin on Erythrocyte Volume and on the Membrane Potential and the Contractile Characteristics of the Extensor Digitorum Longus and Soleus Muscles in Rats

Cited by 28 publications

References 13 publications

In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes

In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes

Oxidoreductase, morphogenesis, extracellular matrix, and calcium ion-binding gene expression in streptozotocin-induced diabetic rat heart

Diabetes Provides an Unfavorable Environment for Muscle Mass and Function after Muscle Injury in Mice

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The Influence of Streptozotocin Diabetes and Metformin on Erythrocyte Volume and on the Membrane Potential and the Contractile Characteristics of the Extensor Digitorum Longus and Soleus Muscles in Rats

Cited by 28 publications

References 13 publications

In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes

In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes

Oxidoreductase, morphogenesis, extracellular matrix, and calcium ion-binding gene expression in streptozotocin-induced diabetic rat heart

Diabetes Provides an Unfavorable Environment for Muscle Mass and Function after Muscle Injury in Mice

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In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes

In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes