The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)

Mateescu, Andreea Lorena; Mincu, Nicolae-Bogdan; Vasilca, Silvana; Apetrei, Roxana-Mihaela; Stan, Diana; Zorilă, Bogdan; Stan, Dana

doi:10.1038/s41598-021-92522-3

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…The use of sugars (e.g., sucrose, glucose, and trehalose) is reported to influence the protein–solvent interaction with a direct effect on both tertiary and quaternary protein structures [ 6 , 7 , 8 ]. Specifically, Lerbret and coworkers observed the “destructuring” effect of trehalose upon the water network by which the water molecules are ordered next to the sugar molecule (as a kosmotrope), preventing ice formation [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase

Gonçalves

Pedro

Oliveira

et al. 2022

IJMS

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Catechol-O-methyltransferase (COMT) has been involved in a number of medical conditions including catechol-estrogen-induced cancers and a great range of cardiovascular and neurodegenerative diseases such as Parkinson’s disease. Currently, Parkinson’s disease treatment relies on a triple prophylaxis, involving dopamine replacement by levodopa, the use of aromatic L-amino acid decarboxylase inhibitors, and the use of COMT inhibitors. Typically, COMT is highly thermolabile, and its soluble isoform (SCOMT) loses biological activity within a short time span preventing further structural and functional trials. Herein, we characterized the thermal stability profile of lysate cells from Komagataella pastoris containing human recombinant SCOMT (hSCOMT) and enzyme-purified fractions (by Immobilized Metal Affinity Chromatography—IMAC) upon interaction with several buffers and additives by Thermal Shift Assay (TSA) and a biological activity assessment. Based on the obtained results, potential conditions able to increase the thermal stability of hSCOMT have been found through the analysis of melting temperature (Tm) variations. Moreover, the use of the ionic liquid 1-butyl-3-methylimidazolium chloride [C4mim]Cl (along with cysteine, trehalose, and glycerol) ensures complete protein solubilization as well as an increment in the protein Tm of approximately 10 °C. Thus, the developed formulation enhances hSCOMT stability with an increment in the percentage of activity recovery of 200% and 70% when the protein was stored at 4 °C and −80 °C, respectively, for 12 h. The formation of metanephrine over time confirmed that the enzyme showed twice the productivity in the presence of the additive. These outstanding achievements might pave the way for the development of future hSCOMT structural and biophysical studies, which are fundamental for the design of novel therapeutic molecules.

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Section: Introductionmentioning

confidence: 99%

Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase

Gonçalves

Pedro

Oliveira

et al. 2022

IJMS

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Relationship between abdominal obesity and C-reactive protein in combination with hypertension in middle-aged and older adults: insights from the CHARLS study

Liao,

Huang,

Wang

et al. 2024

Preprint

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Background Hypertension is a global public health problem, and as good prevention and management of hypertension can effectively reduce the risk of myocardial infarction and stroke, thereby significantly reducing the health economic burden, exploring more risk factors used to identify hypertension remains necessary. Aim To investigate whether the combination of elevated hs-CRP levels and abdominal obesity increases the risk of hypertension in middle-aged and older adults. Methods This study used data from 3439 investigators aged 45 years and older who participated in the 2015 China Health and Retirement Longitudinal Study (CHARLS). Hypertension, waist circumference, and hs-CRP were measured. logistic regression adjusted for confounding variables was used to examine the association between abdominal obesity and hs-CRP under the combination with hypertension. Results The risk of hypertension was significantly increased when participants had both abdominal obesity and high hs-CRP levels. Even after adjusting for covariates, a significant risk of hypertension was still possible (OR = 1.71, 95% CI:1.33–2.20). Sex- and age-stratified analyses showed that the association between abdominal obesity and high hs-CRP levels in combination with incident hypertension was more likely to be observed in participants under 60 years of age (OR = 1.72, 95% CI:1.15–2.59) and in women (OR = 1.72, 95% CI:1.15–2.59). Conclusions The findings suggest that elevated hs-CRP levels in combination with abdominal obesity may increase the risk of hypertensive events in the middle-aged and elderly Chinese population.

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The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)

Cited by 2 publications

References 20 publications

Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase

Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase

Relationship between abdominal obesity and C-reactive protein in combination with hypertension in middle-aged and older adults: insights from the CHARLS study

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