The influence of temperature on antiproliferative effects, cellular uptake and DNA platination of the clinically employed Pt(II)-drugs

Gabano, Elisabetta; Colangelo, Donato; Ghezzi, A.; Osella, Domenico

doi:10.1016/j.jinorgbio.2007.10.006

Cited by 58 publications

(57 citation statements)

References 55 publications

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“…While the final temperature reached through light-induced nanoparticle heating is directly related to the laser power density, the desired temperature for hyperthermia therapy lies in a narrow range, traditionally from 41-43 8C. [17,34] Lower temperatures are therapeutically ineffective, and higher temperatures may cause adverse side effects. Therefore, the measurement of cell suspension temperatures during laser excitation is crucial for the characterization of GNR hyperthermia, and was performed using a thermal camera (Fig.…”

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confidence: 99%

Enhancing the Toxicity of Cancer Chemotherapeutics with Gold Nanorod Hyperthermia

et al. 2008

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confidence: 99%

Enhancing the Toxicity of Cancer Chemotherapeutics with Gold Nanorod Hyperthermia

et al. 2008

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“…Cisplatin interacts with DNA to form DNA intraor inter-strand crosslinks and/or DNA-protein crosslinks, thus induces cytotoxicity (49). Cisplatin in combination with hyperthermia causes an increase in intracellular drug accumulation and induced DNA-platinum adducts (18).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Cisplatin Efficacy by Gold Nanoparticles or Microwave Hyperthermia? An In Vitro Study on a Melanoma Cell Line

et al. 2017

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Purpose: Chemotherapy-resistance of melanoma has led to poor prognosis and decreased survival in the patients. Therefore, the addition of adjuvant therapies to the conventional chemotherapy regimens has been taken into consideration to improve the clinical treatments efficiency. In this study, the effect of microwave (MW) Hyperthermia has been evaluated on the toxicity of cisplatin on the MM200 cell line in the presence and without gold nanoparticles (GNPs). Methods: Cells incubation was performed with and without cisplatin in the presence and absence of GNPs. To induce hyperthermia, the cells were immediately placed under MW irradiation for 25 and 30 minutes (41-43°C) following the addition of the drug and GNPs, then they were incubated for 24 hours. Finally, cell survival was determined by MTT assay.Results: GNPs (up to 6.6 µg/mL) showed no toxicity. GNPs at the concentration of 13.2 and 26.4 µg/mL caused 13% and 20.7% drop in cell survival rate, respectively. IC50 of cisplatin decreased from 4 to 2 µg/mL in the presence of GNPs. Hyperthermia (43°C) plus chemotherapy (2 µg/mL) resulted in no significant enhancement in cisplatin cytotoxicity relative to chemotherapy alone whereas by adding GNPs, an increase in cell mortality up to 15-fold in comparison to cisplatin alone was observed. Conclusions: There is a synergistic effect between cisplatin and GNPs, this could be due to the facilitated entrance of cisplatin in the presence of GNPs. MW exposure improves the efficacy of cisplatin therapy in the presence of GNPs on MM200 cells.

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“…This is probably one of the reasons why some chemotherapeutics will be able to pass the cell barrier more effectively when the cells are treated with hyperthermia. For example, several reports show that the concentration of the chemotherapeutic cisplatin increases in the cell when it is treated with hyperthermia [61][62][63]. Moreover, heat also contributes to structural changes in the membrane by altering the behaviour of membrane-embedded proteins, and this will also increase cellular cisplatin concentrations; this is illustrated by heat facilitating multimerisation of a copper transporter (CTR1) that is responsible for cisplatin uptake [64].…”

Section: Cellular Membrane and Drug Uptakementioning

confidence: 99%

Improving efficacy of hyperthermia in oncology by exploiting biological mechanisms

Tempel¹,

Horsman

Kanaar³

2016

International Journal of Hyperthermia

102

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It has long been established that hyperthermia increases the therapeutic benefit of radiation and chemotherapy in cancer treatment. During the last few years there have been substantial technical improvements in the sources used to apply and measure heat, which greatly increases enthusiasm for the clinical use of hyperthermia. These advances are converging with a better understanding of the physiological and molecular effects of hyperthermia. Therefore, we are now at a juncture where the parameters that will influence the efficacy of hyperthermia in cancer treatment can be optimised in a more systematic and rational manner. In addition, the novel insights in hyperthermia's many biological effects on tumour cells will ultimately result in new treatment regimes. For example, the molecular effects of hyperthermia on the essential cellular process of DNA repair suggest novel combination therapies, with DNA damage response targeting drugs that should now be clinically explored. Here, we provide an overview of recent studies on the various macroscopic and microscopic biological effects of hyperthermia. We indicate the significance of these effects on current treatments and suggest how they will help design novel future treatments. ARTICLE HISTORY

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The influence of temperature on antiproliferative effects, cellular uptake and DNA platination of the clinically employed Pt(II)-drugs

Cited by 58 publications

References 55 publications

Enhancing the Toxicity of Cancer Chemotherapeutics with Gold Nanorod Hyperthermia

Enhancing the Toxicity of Cancer Chemotherapeutics with Gold Nanorod Hyperthermia

Enhancement of Cisplatin Efficacy by Gold Nanoparticles or Microwave Hyperthermia? An In Vitro Study on a Melanoma Cell Line

Improving efficacy of hyperthermia in oncology by exploiting biological mechanisms

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