The influence of testosterone on activity of glycosidases and proteinases in the intestine of the sterlet (Acipenser ruthenus)

The experimental study of the effect of feed additive based on ellagotanins and immobilized probiotic “Ecoflor” on some parameters of non-specific immunity of sterlet Acipenser ruthenus was carried out. Feed additives based on ellagotannins are one of the numerous preparations of natural origin. They have antibacterial and immunostimulating properties. Biologically active additive “Ecoflor” is a consortium of live antagonistically active strains of bifido- and lactobacilli immobilized on enterosorbent. During the experiment, control and experimental groups were kept in flow-through plastic pools. Sterlets were fed 6 times a day. Drugs were added to the feed according to the instructions. Sampling was carried out before the start of the experiment, on 7, 14, 30, 45 and 60 days. Material for the study was taken from 5 individuals from each group. Peripheral blood was taken from the tail vein. After autopsy, tissue pieces of immunocompetent organs (kidney, spleen and liver) were taken from the fish into dry sterile tubes. Blood coagulation and clot retraction were waited for to obtain serum. The serum was then withdrawn with a syringe with a fine needle and transferred into an Eppendorf tube. For further studies, the homogenate was prepared from kidney, spleen and liver with physiological solution in the ratio of 1:6. The level of bacteriostatic activity, C-reactive protein and nonspecific immune complexes was determined in blood serum samples. In homogenates of kidney, spleen and liver the content of immune complexes was determined. The study established high indices of antimicrobial properties of blood serum in all studied fish and the absence of immunodeficient individuals. Lower indices of bacteriostatic activity in experimental groups and zero percent of immunodeficient individuals indicate insignificant influence of preparations on nonspecific antibacterial defense. The majority of the studied fish had a positive level of C-reactive protein, which indicates the presence of inflammatory processes in the body. The content of nonspecific immune complexes in the liver was several times higher than in other tissues, which may be due to the neutralization of components included in the preparations.

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The effect of the probiotic drug "Ecoflor" on the proteolytic activity of the sterlet intestine

Tarleva,

Mikryakov,

Zhandalgarova

2024

jour

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The effect of the probiotic preparation "Ecoflor" on proteolytic activity of the intestinal mucosa and chyme of sterlet was studied. This probiotic is a consortium of strains of lacto- and bifidobacteria: B. bifidum, B. longum and L. acidophilus, L. casei, L. plantarum, immobilized on carbon-mineral sorbent SUMS-1. For the experiment, fish were divided into 4 groups: control and three experimental groups. Experimental groups of fish were given feed with probiotic in the amount of 2, 4, 6 g/kg of feed. Sampling was carried out before the beginning of the experiment, on 7, 14, and 21 days. Material for the study was taken from 5 individuals from each group. To obtain enzymatically active preparations, fish intestines were freed from fat and dried on ice bath glass. After incision, the chyme was collected with a spatula and scraper, then the mucous membrane was removed. The total proteinase activity was calculated by the ratio of the difference between the spectrophotometer readings of the sample with substrate and the blank sample to the product of the intestinal weight (g) by the incubation time (min) EA = ΔE425/(g×min). Determinations were performed in three repetitions. Data analysis showed the stimulating effect of the preparation on proteolytic activity. The maximum increase in enzymatic activity in the experimental groups compared to the control group was recorded on the 7th day of the experiment. A more significant increase in proteolytic activity of the intestinal mucosa compared to chyme was found. At the beginning of feeding, the stimulating effect of the preparation was found in all experimental groups compared to the control. In further observation periods the effectiveness of the probiotic significantly decreased.

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The influence of testosterone on activity of glycosidases and proteinases in the intestine of the sterlet (Acipenser ruthenus)

Cited by 3 publications

References 9 publications

Effect of testosterone on the composition of leucocytes in the peripheral blood and immunocompetent organs of sterlet, Acipenser ruthenus

Effect of testosterone on the composition of leucocytes in the peripheral blood and immunocompetent organs of sterlet, Acipenser ruthenus

Effect of Ellagotanins and Probiotic “Ecoflor” on Sterlet Nonspecific Immunity Indices

The effect of the probiotic drug "Ecoflor" on the proteolytic activity of the sterlet intestine

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