The Influence of the Tretment of Silver Nanoparticles on the Dna of the Nuclei of Thymus Cells, Lymph Nodes and the Follicular Environment of the Oocyte Under Conditions of Experimental Systemic Autoimmune Disorder

T., T. Yu. Voznesenskaya; Stupchuk, M. S.; Grushka, Nataliya; Kondratskaya, O. A.; Blashkiv, T. V.

doi:10.29254/2077-4214-2018-1-2-143-327-331

Cited by 1 publication

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“…Experimental systemic autoimmune disorder (glomerulonephritis) in mice was achieved by immunization of white laboratory mice of the first generation with a kidney antigen suspension derived from a parent. And it was established that DNA damage increased in the nuclei of thymus cells, lymph nodes and cells of follicular environment of the oocyte under experimental glomerulonephritis; the damage on DNA of cells of the thymus and lymph nodes increased under the condition of AgNPs tretment; DNA damage in nuclei of thymus cells, lymph nodes and cells of follicular environment of the oocyte decreased under conditions of experimental glomerulonephritis and tretment of AgNPs [20].…”

Section: Analysis and Discussion Of Resultsmentioning

confidence: 99%

Resumption of Meiotic Maturation of Oocytes, Pre- and Post-Implantational Embryonic Mortality under Conditions of Experimental Glomerulonephritis and Treatment of Silver Nanoparticles

Blashkiv¹,

Stupchuk²,

Sribna³

et al. 2018

Nano BioMed ENG

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The aim of the current study was to estimate the resumption of meiotic maturation of oocytes, preand post-implantation mortality of embryos under conditions of experimental glomerulonephritis and intravenous treatment of silver nanoparticles in mice. Experimental glomerulonephritis in mice was achieved by immunization of white laboratory mice of the first generation with a kidney antigen suspension derived from a parent. The treatment was carried out in the following way: Kidney antigen suspension-intraperitoneal three times 1 time per day; the procedure was repeated in 3 weeks, one time intraperitoneally with the same dose (10 mkL of suspension per 10 grams of body weight of the animal). Silver nanoparticles (AgNPs, 30 nm)-intravenous treatment three times: 1 time per day for 1 h before immunization of animals with suspension of kidney antigen; as well as in 3 weeks once with the same dose (2 mg/kg). Thus, the number of oocytes that resumed meiosis in vitro from animals under experimental glomerulonephritis decreased as compared to the numbers in control animals; no inhibition of meiotic resumption of oocytes of animals was established under conditions of AgNPs treatment; the AgNPs treatment under conditions of experimental glomerulonephritis increased the number of oocytes that resumed meiosis in vitro compared with such values in the control and under conditions of experimental glomerulonephritis. The value of post-implantation embryonic mortality increased under conditions of experimental glomerulonephritis; no infertility of implantation in females of mice under conditions of AgNPs treatment; the AgNPs treatment under conditions of experimental glomerulonephritis decreased post-implantation mortality of embryos.

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Section: Analysis and Discussion Of Resultsmentioning

confidence: 99%