ŠKARPA, P.: Effect of soil nutrients reserves and level of fertilisation on production parameters of spring barley (Hordeum vulgare L.). Acta univ. agric. et silvic. Mendel. Brun., 2006, LIV, No. 4, pp 91-98 The effect of three different doses of basic fertilisers and a subsequent pre-sowing supplementary fertilisation on production parameters (yield of grain, number of spikes, and thousand grains weight) was evaluated using experimental data obtained within the framework of a one-year pot experiment with spring barley (Hordeum vulgare L.) established at the Department of Agrochemistry and Plant Nutrition, Faculty of Agronomy, Mendel University of Agriculture and Forestry Brno in 2003. Results of statistical analysis indicated that the yield of grain was signifi cantly infl uenced by different doses of fertilisers especially on sandy soils. As compared with control, the second highest dose of fertilisers (i.e. 83 kg N, 31 kg P and 92 kg K.ha -1 ) increased the yield by 91.7 % and the third one (i.e. 113 kg N, 43 kg P and 125 kg K.ha -1 ) even by 124.8 %. This increase in the grain yield was positively affected above all by increasing doses of nitrogen fertilisers. A pre-sowing application of P, K and Mg showed also a positive effect on grain yield not only on sandy but above all on clay soils (as compared with non-fertilised control, this increase ranged from 40.6 to 50.2%). Fertilisation showed also a marked effect on the number of spikes. This factor showed a similar trend as the yield of grain. The thousand grains weight was not signifi cantly infl uenced on both soil types. This value was increased (by 2.9% to 14.8%) after the application of fertilisers prior to sowing but the difference was statistically non-signifi cant.