The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour

Ramires, P. A.; Romito, Antonio; Cosentino, Francesco; Milella, E.

doi:10.1016/s0142-9612(00)00269-6

Cited by 279 publications

(172 citation statements)

References 29 publications

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“…Thus, gene expression from composite HA/TiO2 coatings may be hard to interpret due to gene responses to both HA and the TiO2 oxide layer. Nevertheless, surface-specific changes in osteogenic bone-associated gene expression were observed in human osteoblasts, which may mediate, at least partly, the later changes in cell proliferation and differentiation observed in this work and in literature [13][14][15].…”

Section: Discussionmentioning

confidence: 83%

“…While no significant change was found in proliferation, marked changes in ALP activity were found with such HA/TiO2 coated surfaces [13]. Ramires et al (2001) [14] also found altered expression of other differentiation markers at 7 days using the MG63 osteoblast-like cell line, and confirmed these findings with rat primary osteoblasts [15], although different HA/TiO2 compositions and manufacture methods were utilized. An absence of cytotoxic effects for HA/TiO2 sol-gel coatings, assessed by viability assays in material extracts at 3 days, was also reported [15].…”

Section: Introductionmentioning

confidence: 80%

“…Previous work on such sol-gel composites has suggested optimum compositions of HA+20% TiO2 [13] and HA+50% TiO2 [14], as determined by differentiation marker production in immortal or animal cells. This work, assessing earlier cell functions and behaviour using human primary cells, proposes that a lower composition of TiO2 may be more biologically optimal in such composite coatings, although decreased TiO2 composite levels may not be practical in vivo due to their adverse affect on coating adhesion strength [13] The early expression of osteogenic genes by all surfaces was additionally studied.…”

Section: Discussionmentioning

confidence: 99%

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Initial responses of human osteoblasts to sol–gel modified titanium with hydroxyapatite and titania composition

et al. 2006

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Initial responses of human osteoblasts to sol–gel modified titanium with hydroxyapatite and titania composition

et al. 2006

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“…Yellow tetrazolium salt (MTT) is reduced to purple formazan in the living cells or cells in early apoptosis cycle with active mitochondrial dehydrogenases [16,17]. Therefore, it is important to note that changes in cell metabolic activity may cause significant changes in formazan production, despite the constant number of viable cells [18].…”

Section: +mentioning

confidence: 99%

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

et al. 2013

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Calcium phosphate bioceramics have been studied as bone filler materials for years and have become a component of many commercial products. It is widely known that surface-reactive biomaterials may cause changes in the concentration of crucial ions in the surrounding environment, thereby affecting cell metabolism and viability. The aim of this study was to produce five cement-type biomaterials and characterize their phase composition using X-ray diffraction method, and porosity and pore size distribution using mercury intrusion porosimeter. We then evaluated ion interactions of the novel biomaterials with the surrounding environment (culture medium). A commercially available bone substitute, HydroSet™ (Stryker®), was used as a reference. MTT and NRU cytotoxicity tests were performed to assess the effect of changes in the concentration of crucial ions (calcium, magnesium, phosphate) on osteoblast metabolism and viability in vitro. Our study clearly indicated that various biomaterials demonstrated different ion reactivity and consequently may cause changes in ion concentration in the local environment. Critically low or high values of calcium, magnesium, and phosphate concentrations in the medium exerted cytotoxic effects on the cultured cells. Moreover, we discovered that the chemical composition of the culture medium had a substantial influence on ion interactions with biomaterials.

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“…The TiO 2 + 15 vol.% HA composite rods showed an increase of shear strength of 1.7 times when compared to that of the pure TiO 2 rods. Ramires et al [15] The objective of this work was to HVOF spray nanostructured TiO 2 , nanostructured TiO 2 + 10 wt% HA and nanostructured TiO 2 + 20 wt% HA feedstock powders, in order to engineer coatings having the good mechanical performance of the HVOFsprayed nanostructured titania and the proven biocompatibility of HA. In order to try to separate the effects of powder composition and morphology on the microstructure and mechanical performance of the coatings from that of the in-flight characteristics of the particles during thermal spraying, the feedstock powders were engineered to exhibit a similar particle size distribution and were thermally sprayed by choosing spray parameters that produced similar values of particle temperature and velocity in the HVOF jet.…”

Section: Titania (Tio 2 ) As a Biomedical Coatingmentioning

confidence: 99%

Nanostructured titania/hydroxyapatite composite coatings deposited by high velocity oxy-fuel (HVOF) spraying

Gaona

Lima

Marple

2007

Materials Science and Engineering: A

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Nanostructured titania/hydroxyapatite composite coatings deposited by high velocity oxy-fuel (HVOF) spraying Gaona, M.; Lima, R. S.; Marple, B. R.Contact us / Contactez nous: nparc.cisti@nrc-cnrc.gc.ca. AbstractPure nanostructured titania (TiO 2 ) and blends with 10 and 20 wt% hydroxyapatite (HA) powders were sprayed onto Ti-6Al-4V substrates using a high velocity oxy-fuel (HVOF) system. The feedstock powders employed in this work were engineered to exhibit similar particle size distributions in order to generate similar values of particle temperature and velocity in the spray jet. By achieving these characteristics it was assumed that the differences in coating properties and microstructures produced in this study were mainly related to the nature and composition of the feedstock powders, rather than to the spraying parameters or in-flight particle characteristics. The microstructure, porosity, roughness, Vickers hardness and bond strength (ASTM C633) of these coatings were analyzed and evaluated. X-ray diffraction (XRD) patterns showed that no detectable chemical reaction occurred between the nanostructured TiO 2 and HA phases during the spray process. Due to the poor mechanical performance of HA, its addition decreased the bond strength and hardness values of the coatings, especially when the content of HA was 20 wt%; however, the bond strength values were still much superior to those of HA thermally sprayed coatings. The addition of HA to nanostructured titania for producing HVOF-sprayed coatings could be very interesting for biomedical applications due to the combination of the good mechanical performance and chemical stability of nanostructured titania and a bioactive phase (HA) that can enhance the bio-performance of the coating. Crown

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The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour

Cited by 279 publications

References 29 publications

Initial responses of human osteoblasts to sol–gel modified titanium with hydroxyapatite and titania composition

Initial responses of human osteoblasts to sol–gel modified titanium with hydroxyapatite and titania composition

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

Nanostructured titania/hydroxyapatite composite coatings deposited by high velocity oxy-fuel (HVOF) spraying

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