The Influence of Titanium Surfaces in Cultures of Neonatal Rat Calvarial Osteoblast-Like Cells: An Immunohistochemical Study

Aybar, Büket; Emes, Yusuf; Atalay, Belir; Tanrikulu, Sinasi H.; Kaya, Ayşem; İşsever, Halim; Ceyhan, Taşk ̇in; Bılır, Ayhan

doi:10.1097/id.0b013e31818c5bd8

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…This cell morphology on the surface of SLA was consistent with that observed by Aybar et al 19. In the SEM images of the SLActive group at a high cell count, few squamous cells were seen.…”

Section: Discussionsupporting

confidence: 91%

“…In our study, the high cell count after 72 h despite a decrease in DNA activity suggested that rapid cell proliferation occurred in the early phase. Aybar et al 19 reached the same conclusion.…”

Section: Discussionmentioning

confidence: 59%

“…Aybar et al 19 investigated DNA synthesis in osteoblasts using BrdU analysis. Mustafa et al 11 and Aybar et al used BrdU analyses to evaluate cellular DNA activity in their studies examining the surface properties of titanium discs.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Light-Emitting Diode (LED-660 Nm) Application over Primary Osteoblast-Like Cells on Titanium Surfaces: An In Vitro Study

Çankaya¹,

Erdem²,

Erdem³

et al. 2011

Int. J. Med. Sci.

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Background: The goal of this study was to evaluate the behavior of neonatal rat calvarial osteoblast-like cells cultured on different implant surfaces and exposed once or three times to a 660-nm light-emitting diode (LED).Methods: An LED with a 660-nm wavelength was applied once or three times to cultured cells on standard and modified sandblasted acid-etched surfaces (SLA and SLActive; Straumann, Basel, Switzerland). To analyze the effect of the LED on cell proliferation, numbers, and viability, cells were cultured on titanium discs, and measurements were taken after 72 h. Cell proliferation rates were assessed using a bromodeoxyuridine immunohistochemical technique. Cell morphologies were evaluated by scanning electron microscopy (SEM).Results: Osteoblast-like cells proliferated on all tested surfaces, with differences among groups in cell counts and DNA synthesis values. The application of one LED treatment caused a significant increase in cell count in the SLActive group in comparison with the SLA group (p = 0.001), whereas the application of three LED treatments caused a significant decrease in cell count in the SLA group compared with the SLActive group (p < 0.001). After 72 h, the number of cells was highest in the SLActive group exposed once to the LED.Conclusions: One LED application in the SLActive group resulted in significantly increased cell numbers. However, these findings were not exactly compatible with the SEM findings, which demonstrated fewer cells and weak attachments between cells and to the surface. Thus, further studies using different LED application times are needed to clarify the reason for the increased number of cells that are apparently incapable of attaching to the titanium surfaces after 72 h.

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“…This cell morphology on the surface of SLA was consistent with that observed by Aybar et al 19. In the SEM images of the SLActive group at a high cell count, few squamous cells were seen.…”

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 59%