The influence of topical mitomycin-C on total fibroblasts, epithelialization, and collagenization in anoplasty wound healing in Wistar rats

Lampus, Harsali; Kusmayadi, Dikki Drajat; Nawas, Bustanul Arifin

doi:10.1016/j.jpedsurg.2015.03.059

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…In clinical practice, it is very di cult to reliably assess the role of these factors in treatment outcome, given the relatively small number of children admitted to a single clinical center and signi cant variability factors affecting the treatment outcomes. Researchers have previously created models similar to ours, however, the impairment of rectal vascularization due to rectal mobilization was not standardized and assessment of rectal ischemia and rectal tissue tension were not described [15]. There is a study in which researchers performed anorectal mobilization followed by 2 and 4 cm resection of the rectum in dogs to evaluate the role of the IAS in fecal continence.…”

Section: Discussionmentioning

confidence: 99%

Effects of extensive mobilization and tension anastomosis in anorectal reconstruction (experimental study)

et al. 2022

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Anorectoplasty and pull-through procedure can be performed with extended mobilization or tension anastomosis, which can compromise the vasculature of the rectum. We aimed to analyze the histopathological ndings, hypoxia biomarker values and to correlate the incidence of anal stenosis and defecation disorders of both conditions in experimental models. MethodsWe created anorectal reconstruction models during anorectal mobilization with extended mobilization with impaired vascularization (group I) and tension anastomosis (group II) in rats. Hypoxia biomarker values was assessed in both groups and in sham operated animals (group III). The histopathological changes on the 3 rd postoperative day, anal stenosis and defecation disorders on the 35 th day are compared with each other and with a control group (group IV). ResultsHypoxia biomarker levels con rmed postoperative ischemia in I -III groups compared to the control.Groups I, II are accompanied by pronounced histopathological changes in the anorectum on the 3rd postoperative day and accompanied by severe brosis on the 35 th day. In comparison to group III, both groups showed defecation disorders and anal stenoses. ConclusionExtensive mobilization with vascular impairment and tension anastomosis resulted in similar ischemia with histopathologic changes, which in the long term resulted in brotic changes associated with defecation disorders.

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Section: Discussionmentioning

confidence: 99%

Effects of extensive mobilization and tension anastomosis in anorectal reconstruction (experimental study)

et al. 2022

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“…Lampus et al [16] investigated 12 Wistar rats undergoing anoplasty in two groups, one of which was the control group. While one group underwent the anoplasty procedure only, the other group was administered 0.5 mg/mL MMC topically for five minutes following the anoplasty procedure.…”

Section: Discussionmentioning

confidence: 99%

Effect of mitomycin-C applied through different approaches following tracheal surgery on development of granulation tissue and level of nephrotoxicity in rats

Kuzucuoğlu

Topuz

Altun

2019

Turk Gogus Kalp Dama

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Bu çalışmada trakea cerrahisi uygulanan sıçanlarda farklı ilaç uygulama yaklaşımları ile uygulanan mitomisin-C'nin cerrahi bölgede granülasyon dokusu gelişimi ve böbrek fonksiyonları üzerine etkisi değerlendirildi.Ça lış m a pl a nı: Elli erkek erişkin Sprague Dawley sıçan (ağırlık ort. 200 g-300 g) beş gruba ayrıldı. Tüm gruplara anestezi altında trakeanın beşinci ve altıncı kıkırdak halkası arasından kesi yapıldı ve kesi 6/0 monofilaman emilebilir dikiş ile primer olarak onarıldı. Deney hayvanlarına atandıkları topikal, intraperitoneal enjeksiyon, yara kenarlarına enjeksiyon ve inhalasyon yolu gruplarına uygun olarak bir doz 0.5 mg mitomisin-C uygulandı. Kontrol grubu kabul edilen bir gruba mitomisin-C uygulanmadı. Cerrahiden dört hafta sonra sıçanlar sakrifiye edildi ve takiben trakeaları çıkarıldı. Trakea dokuları histopatolojik olarak epitelizasyon, fibrozis, fibroblast miktarı, angiogenezis ve enflamatuar yanıt açısından değerlendirildi. Trakea çapı ve duvar kalınlığı ölçüldü. Kan üre ve kreatinin değerleri nefrotoksisite açısından değerlendirildi ve sıçanlar immunhistokimyasal olarak glomeruler patoloji açısından incelendi.Bul gu lar: Kontrol grubuna kıyasla topikal mitomisin-C uygulanan grupta epitelizasyon istatistiksel olarak anlamlı derecede yavaşladı (p<0.01), trakea çapı istatistiksel olarak anlamlı derecede daha genişti (p<0.05) ve trakea duvar kalınlığı anlamlı olarak daha kalındı (p<0.01). So nuç:Trakea cerrahisi sonrası topikal olarak uygulanan mitomisin-C epitelizasyonu yavaşlatarak granülasyon dokusu gelişimini azaltmakta ve trakea çapının daha geniş kalmasını sağlamaktadır.

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“…The study by Gray et al 14 reported that mitomycin-c has the ability to inhibit DNA synthesis to prevent the proliferation of fibroblasts in the long term. Experimental study on animals conducted by Lampus et al 15 reported that topical mitomycin-c was able to reduce total fibroblast in anoplasty wound healing trials in Wistar rats. Another study by Su et al 16 also shows the same result, finding topical MMC over 0.3 mg/mL can delay the wound healing via anti-cell proliferation effect and also that MMC inhibits angiogenesis of the wound via inhibition of vascular endothelial growth factor (VEGF).…”

Section: Discussionmentioning

confidence: 99%

Chronic wound mitomycin-c-induced animal models

et al. 2018

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A chronic wound is a problem often encountered in medical care, especially in areas that do not have adequate health facilities. Several factors causing the injury are mechanical, chemical, electrical, or heat. Chronic inflammation and bacterial infections are two significant factors that affect the process of wound chronicity. Mitomycin-C (MMC) is widely used as intravenous, oral, or topical anti-cancer drug. Mitomycin-C that is topically administration to an injury can cause cross-linking and decrease or stop the DNA transcription process lead the injury will not reach the healing phase. Mitomycin-C works as an inhibitor of fibroblast proliferation that can inhibit wound healing process. This study aimed to investigate the effect of topical MMC administration in chronic wound animals model. Eight female Wistar rats aged 70 to 90 days and weighing between 300 to 350g were used in this study. Wound model was made in the back area with a diameter of approximately 2 cm. Rats were then divided into 2 groups. The first group as treatment group which the wound rats were compressed using sterile gauze moistened with 0.5 mg/mL of MMC for five min and rinsed with 10 mL of saline solution. The second group as control which the wound rats were compressed by using sterile gauze moistened with saline for 5 minutes. On day 3, 5, and 15, the wound profiles were observed consisting of wound diameter, necrosis, and consistency. The rat wound after MMC administration showed a slower decrease in diameter, fewer scar tissue formation, and development of necrotic tissue. In addition, the rat wound appeared as brownish-black, dry, thick chronic wounds and took longer to heal compared to those saline control. In conclusion, MMC administration inhibits the wound healing process on rat surgical wound model.

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The influence of topical mitomycin-C on total fibroblasts, epithelialization, and collagenization in anoplasty wound healing in Wistar rats

Cited by 5 publications

References 22 publications

Effects of extensive mobilization and tension anastomosis in anorectal reconstruction (experimental study)

Effects of extensive mobilization and tension anastomosis in anorectal reconstruction (experimental study)

Effect of mitomycin-C applied through different approaches following tracheal surgery on development of granulation tissue and level of nephrotoxicity in rats

Chronic wound mitomycin-c-induced animal models

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