The influence of total solids content and initial pH on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes

Robledo-Narváez, Paula Natalia; Muñoz-Páez, Karla M.; Poggi‐Varaldo, Héctor M.; Rı́os-Leal, Elvira; Calva-Calva, Graciano; Ortega‐Clemente, Luis Alfredo; Rinderknecht-Seijas, N.; Estrada-Vázquez, Carlos; Ponce-Noyola, M.T.; Salazar-Montoya, Juan Alfredo

doi:10.1016/j.jenvman.2013.04.042

Cited by 42 publications

(30 citation statements)

References 89 publications

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“…The negative influence of high solids content has also been found in other studies. For example, in one study, it was found that the H 2 production was negatively influenced by total solid contents above 18% using digestates from a methanogenic solid substrate anaerobic digester (Robledo-Narváez et al, 2013). The reason for the increased rates without solids could be related to the improved mass transfer and higher water activity.…”

Section: Fermentation Using the Hydrolysate Juice As Feed-stockmentioning

confidence: 99%

Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Sjöblom

Μάτσακας

Christakopoulos

et al. 2015

Industrial Crops and Products

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a b s t r a c tEnzymatically liquefied sweet sorghum stalks and beet molasses were evaluated for butyrate production using Clostridium tyrobutyricum in 1 L scale fed-batch fermentations. The hydrolysates used for the fermentations were prepared separately by liquefying the sorghum stalks at 50 • C, pH 5.0 for 18 h, with 30% (w/v) DM content using the enzyme preparation Cellic ® CTec2 at an activity of 26.5 FPU/g DM. To enhance sucrose consumption, the fermentations were supplemented with invertase at an activity equivalent to 8.3 U/g DM. With the hydrolysate as the feedstock, a butyrate concentration of 37.2 ± 0.8 g/L, a productivity of 0.86 ± 0.02 g/L h and a yield of 0.39 ± 0.02 g/g (p = 0.05) consumed sugars were obtained. Finally, a butyrate concentration of 58.8 g/L, a productivity of 1.9 g/L h, a butyrate yield of 0.52 g/g consumed sugars and a dry cell mass concentration of 15.7 g/L were obtained with fed-batch cultivation and a constant feed consisting of 64% sorghum hydrolysate juice and 36% molasses. Evidence for inducible saccharolytic activity was also proven, as the cellulase activity in the culture supernatant was found more than double during feed with limiting sugar concentrations. The present study clearly demonstrates that combinations of low cost raw materials can be used for efficient butyrate production, also without cell immobilization.

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Section: Fermentation Using the Hydrolysate Juice As Feed-stockmentioning

confidence: 99%

Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Sjöblom

Μάτσακας

Christakopoulos

et al. 2015

Industrial Crops and Products

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“…So far, the effect of TS content on dry microbial fermentation has rarely been evaluated. Only two studies compared the effects of a gradient of TS content (from 21 to 35% TS [13] and from 10 to 35% TS [14]) on biohydrogen production. Both concluded to a decrease of hydrogen production with an increase of TS content and the importance of alkalinity to buffer the media.…”

Section: Introductionmentioning

confidence: 99%

Total solids content: a key parameter of metabolic pathways in dry anaerobic digestion

Motte

Trably

Escudié

et al. 2013

Biotechnol Biofuels

141

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BackgroundIn solid-state anaerobic digestion (AD) bioprocesses, hydrolytic and acidogenic microbial metabolisms have not yet been clarified. Since these stages are particularly important for the establishment of the biological reaction, better knowledge could optimize the process performances by process parameters adjustment.ResultsThis study demonstrated the effect of total solids (TS) content on microbial fermentation of wheat straw with six different TS contents ranging from wet to dry conditions (10 to 33% TS). Three groups of metabolic behaviors were distinguished based on wheat straw conversion rates with 2,200, 1,600, and 1,400 mmol.kgVS-1 of fermentative products under wet (10 and 14% TS), dry (19 to 28% TS), and highly dry (28 to 33% TS) conditions, respectively. Furthermore, both wet and dry fermentations showed acetic and butyric acid metabolisms, whereas a mainly butyric acid metabolism occurred in highly dry fermentation.ConclusionSubstrate conversion was reduced with no changes of the metabolic pathways until a clear limit at 28% TS content, which corresponded to the threshold value of free water content of wheat straw. This study suggested that metabolic pathways present a limit of TS content for high-solid AD.

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“…They found that there was a considerable increase of volatile fatty acids (VFA, also known as short chain organic acids) compared with the non‐pretreated WAS (2243 mg COD L −1 vs. 191.1 mg COD L −1 , respectively). Regarding this, further studies are necessary because on the one hand, VFA accumulation can be inhibitory to further bioH 2 , and on the other hand, generation of some VFAs, for instance, acetate, are associated with high yields of biohydrogen during dark fermentation of wastes . It is known that some genuses of Clostridia are able to ferment hexose to 4 moles of H 2 plus acetate (Equation (1)), which is the highest yield of H 2 from hexoses in DF whereas microorganisms engaged in mixed fermentation and those that give butyrate as the fermentation product of hexoses will generate 2 moles of H 2 or less (Equation (2)).…”

Section: Classification Of Pretreatmentsmentioning

confidence: 99%

“…Several factors have significant effects on DF from organic substrates. For instance, a high solids content or low pH (<4.5) or high lactic acid and solvents production, as well as other fermentation deviations, negatively affect the hydrogen productivity from the OFMSW as feedstock . Furthermore, other factors such as alkalinity, the type of inoculum and method of inoculum conditioning and enrichment, the method of methanogenesis inhibition used, and hydraulic retention time are also important .…”

Section: Introductionmentioning

confidence: 99%

A review of the potential of pretreated solids to improve gas biofuels production in the context of an OFMSW biorefinery

Romero-Cedillo

Poggi‐Varaldo

Ponce-Noyola³

et al. 2016

J. Chem. Technol. Biotechnol.

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The organic fraction of municipal solid waste (OFMSW), mainly composed of lignocellulosic polymers, is extremely complex. Therefore, it is necessary to apply pretreatments to remove the lignin content and decrease the cellulose crystallinity in order to use the OFMSW for gas biofuels production in the context of biorefineries from waste. This work focused on critically reviewing the conventional pretreatments applied to OFMSW, with the goal of improving the H 2 production, as well as other biofuels in modern biorefineries. There are a wide variety of pretreatments that have successfully been used, mainly alkaline, milling and dilute acid. In addition, some research has focused on the recovery and reutilization of the alkali, acid or solvents after the pretreatment, to be incorporated into new cycles of production, minimizing the environmental impacts. Moreover, it would be necessary to incorporate analytical tools, in order to determine the sustainability of the biorefinery project. It is concluded that waste pretreatments could significantly contribute to increased yields of biogas fuels in organic waste-based biorefineries. Therefore, establishing preliminary stages for conditioning biomass or wastes is essential to improve the degradation of wastes and bio-product generation. 938 www.soci.org L Romero-Cedillo et al. db dry basis wb wet basis wrm wet reactor mass J Chem Technol Biotechnol 2017; 92: 937-958 /jctb , [56.76 j , 2.53 k ] 104 J Chem Technol Biotechnol 2017; 92: 937-958 Steam explosion Steam explosion, also known as hydrothermal, promotes the formation of soluble lignin compounds, such as HMF, due to high temperatures that stimulate the breakdown of sugars. 121 In this process, HMF and other inhibitor compounds are generated, such as furans from pentoses and HMF from hexoses. 122 Interestingly, HMF can be finally degraded into levulinic and formic acids. In contrast, even when a pretreatment did not necessarily remove lignin, it was hypothesized that high temperatures had a significant J Chem Technol Biotechnol 2017; 92: 937-958 /jctb J Chem Technol Biotechnol 2017; 92: 937-958

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The influence of total solids content and initial pH on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes

Cited by 42 publications

References 89 publications

Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses

Total solids content: a key parameter of metabolic pathways in dry anaerobic digestion

A review of the potential of pretreated solids to improve gas biofuels production in the context of an OFMSW biorefinery

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