The influence of various cell kinetic conditions on functional differentiation in the small intestine of the rat

Both, N.J. de; Dongen, J.M. van; Hofwegen, B. van; Keulemans, J. L. M.; Visser, W.J.; Galjaard, H.

doi:10.1016/0012-1606(74)90263-2

Cited by 43 publications

(20 citation statements)

References 38 publications

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“…In proliferating crypt cells, lacking the microvillous structure [24], the enzyme activity is about 15% of the upper villous cell activity (table 1). The preferential localization in the brushborder may explain the high specific activity of this particulate enzyme in mutosal scrapings as compared to most other tissues of the rat in which microvillous structures are less well developed or nearly absent (compare [2]).…”

Section: Discussionmentioning

confidence: 99%

The localization of guanylate cyclase in rat small intestinal epithelium

Jonge

1975

FEBS Letters

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Section: Discussionmentioning

confidence: 99%

The localization of guanylate cyclase in rat small intestinal epithelium

Jonge

1975

FEBS Letters

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“…(3) Reference system: in the experiments of ae Both et al (7) and Menge et al (28), significant changes in villus height were not accompanied by corre sponding alterations in disaccharidase activity referred to protein. In tírese situa tions and perhaps in the present experiments, changes in enzyme activity were accompanied by similar alterations in the reference system (mucosal protein) and as a consequence of this, the specific activity of the enzyme remained unaltered.…”

Section: Discussionmentioning

confidence: 99%

The Effect of a Single Dose of Cyclophosphamide on the Jejunum of Specified Pathogenfree and Germfree Rats

Ecknauer¹,

Löhrs²

1976

Digestion

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After a single dose of cyclophosphamide (100 mg/kg) cell proliferation in the jejunum of the rat decreased within the first 24 h and returned to the initial level after 48 h. Under the influence of cyclophosphamide, an increased cell loss in germfree rats could be observed. Villus height and villus cell count tended to decrease. Changes in disaccharidase activity in mucosal scrapings with respect to protein and DNA content could not be demonstrated. An influence of presence or absence of bacterial flora could be observed.

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“…The enzyme activities were estimated for each animal in ten samples of crypt-material and ten samples of villus-material. The non-specific esterase served as a parameter for crypt cell maturation and the alkaline phosphatase, leucine aminopeptidase and neutral a-glucosidase as parameters for villus cell function, this distinction being based on earlier experiments (de Both et al, 1974;Galjaard et al, 1970Galjaard et al, , 1972b.…”

Section: Enzymatic Assaysmentioning

confidence: 99%

“…The effect of changes in crypt cell proliferation on the process of cell maturation has also been investigated by microchemical enzyme analyses on isolated crypts and villi, during continuous irradiation and during a subsequent recovery period. The activity of the non-specific esterase was measured as aparameter for crypt cell maturation (de Both et al, 1974;Galjaard, van Duuren & Giesen, 1970;van der Meer-Fieggen, 1973) and the activities of alkaline phosphatase, leucine aminopeptidase and neutral cc-glucosidase were analysed as parameters for villus cell function (de Both et al, 1974;Fortin-Magana et al, 1970;Galjaard, van der Meer-Fieggen & de Both, 1972b;Nordstrom, Dahlqvist & Josefsson, 1968;Webster & Harrison, 1969).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Continuous Irradiation on Cell Proliferation and Maturation in Small Intestinal Epithelium

et al. 1975

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Autoradiographic studies and scintillation counting of crypt material after pulse labelling with 3H‐thymidine showed that during continuous irradiation with 290 rads/day a reduced proliferative activity is present in the crypts of rat small intestine after 1 day of irradiation and of normal activity during the remaining period (5 days) irradiation. After cessation of irradiation an increase in proliferative activity can be observed after 1 day of recovery. From the time (36–48 hr after starting of the irradiation) that the number of villus cells is reduced an expansion of the proliferation zone in the crypt was observed. Both effects last until 1 day of recovery after cessation of irradiation. The process of crypt cell maturation and of villus cell function has also been studied during and after continuous irradiation by micro‐chemical enzyme analyses in isolated crypts and villi. It was found that the expansion of the proliferation zone in the crypt is accompanied by a decrease in activity of only those enzymes (i.e. non‐specific esterases) which normally become active during crypt cell maturation. The activity of enzymes normally present mainly in the functional villus cells remained relatively unaffected by changes in crypt cell kinetics. A hypothesis of different regulation mechanisms of the proliferative activity in the intestinal crypt and a possible explanation of the different behaviour of various enzyme activities as a result of changes in crypt cell proliferation is discussed.

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The influence of various cell kinetic conditions on functional differentiation in the small intestine of the rat

Cited by 43 publications

References 38 publications

The localization of guanylate cyclase in rat small intestinal epithelium

The localization of guanylate cyclase in rat small intestinal epithelium

The Effect of a Single Dose of Cyclophosphamide on the Jejunum of Specified Pathogenfree and Germfree Rats

The Effect of Continuous Irradiation on Cell Proliferation and Maturation in Small Intestinal Epithelium

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