The influence of water structure on the rotational depolarization of fluorescence

Collisional Quenching of Erythrosine B as a Potential Reference Dye for Impulse Response Function Evaluation

“…7,8 The authors showed that the lifetime of ErB is slightly dependent on temperature and equals approximately 80 ps. The weak dependence of ErB fluorescence was also reported by Matczuk et al 9 In fact, the lifetime of ErB slightly increases with the temperature. Later, the lifetimes of ErB were measured in both the frequency domain and the time domain and the recently reported values are slightly higher, 89 ps in water and 480 ps in methanol.…”

Accurate anisotropy recovery from fluorophore mixtures using Multivariate Curve Resolution (MCR)

“…These concentrations yielded equivalent fluorescence intensities and minimized IFE [53]. In addition, the short lifetimes (for example 75 ps for Erythrosin B in water [54]) of these fluorophores should further minimize depolarization effects. The absorbance and the uncorrected fluorescence EEM spectra (Figure 4) of Phloxine B and Erythrosin B show that there is a small degree of IFE for EB, expected because of the relatively high absorbance (~ 0.18) for 6 μM EB compared to (~ 0.019) for 0.6 μM PB.…”

Photo-catalytic H2 evolution, structural effect and electron transfer mechanism based on four novel [2Fe2S] model complexes by photochemical splitting water

“…where I 0 is the initial fluorescence emission intensity in the absence of quencher, I is the corresponding value in the presence of quencher, τ 0 is the excited-state lifetime in the absence of quencher (for EBS 2-, τ 0 =75ps) (Matczuk, et al, 1995), k q is the bimolecular quenching rate constant, and [Q] is the molar concentration of quencher.…”