In this study, six grip spans (45mm~65mm) were tested to evaluate the effects of handle grip span and user's hand size on maximum grip strength, individual finger force, and subjective ratings of comfort using a digital dynamometer with individual force sensors. Forty-six males were assigned into three hand size groups according to their hand lengths. Results showed that overall 55mm and 50mm grip spans were the most comfortable sizes and associated with the highest grip strength in the maximum grip force exertions, whereas 65mm grip span was rated as the least comfortable size as well as the lowest grip strength. In the interaction effect of grip span and hand size, small and middle hand sized participants rated the best preference and the least preference grip spans differently with large hand sized participants. With respect to the analysis of individual finger force, the middle finger force was the strongest and the highest contribution to the total finger force, followed by ring, index and little fingers. In addition, it was noted that each finger had a different optimal grip span for exerting maximum force resulting in a bowed contoured shaped handle for two-handle hand tools. Thus, the grip spans for two-handle hand tools might be designed according to the users' hand and finger anthropometrics to maximize performance and subjective perception of comfort.