The inhibition of lipid synthesis in vitro in the locust, Schistocerca gregaria, by factors from the corpora cardiaca

Gokuldas, M.; Hunt, Philip A.; Candy, David J.

doi:10.1111/j.1365-3032.1988.tb00907.x

Cited by 56 publications

(28 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1b), which can be interpreted as a preparation to fuel vitellogenic oocyte growth. High acetate incorporation into fat body lipids that precedes the onset of vitellogenic oocyte growth has also been shown for locusts (Gokuldas et al, 1988;Lee and Goldsworthy, 1995) and mosquitoes (Ziegler, 1997). Mosquitoes are capable of converting dietary sugars into lipid reserves during the first days of adult life that are later used to support egg production (Van Handel, 1965;Ziegler and Ibrahim, 2001).…”

Section: Discussionmentioning

confidence: 90%

Changes in the biochemical composition of fat body stores during adult development of female crickets, Gryllus bimaculatus

Lorenz

Anand

2004

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Age-dependent changes in the fat body composition and aspects of lipogenesis in the free abdominal fat body of female crickets, Gryllus bimaculatus, were studied. Lipid, protein, glycogen, and free carbohydrate content of the fat body, and fat body wet weight increased simultaneously and sharply from day 0 onwards and were doubled/almost doubled by day 2 after adult emergence. Lipogenic activity of the fat body, fat body weight, and the energy stores in the fat body peaked on day 2, except for free carbohydrate, which peaked on day 3. On day 2, the fat body was mainly comprised of lipid (53.8%) and protein (6.6%), while glycogen and free carbohydrate together contributed less than 1% of the fat body wet weight. After peaking, both lipogenesis and energy stores decreased in a synchronous manner. The depletion of the fat body energy stores and the consequent decrease in the fat body weight were concomitant with a fast and massive gain in ovary weight (day 2: 19.5 +/- 1.5 mg; day 4: 332.8 +/- 31.5 mg) due to the vitellogenic oocyte growth that started on day 2. Our data clearly underline the importance of the free abdominal fat body as a source of energy for reproduction in the cricket. Fat body fatty acid synthase activity coincided with lipogenic activity. Adipokinetic hormone inhibits lipid synthesis in the fat body, but treatment of the fat body with adipokinetic hormone in vitro showed no consistent effect on fatty acid synthase activity.

show abstract

Section: Discussionmentioning

confidence: 90%

Changes in the biochemical composition of fat body stores during adult development of female crickets, Gryllus bimaculatus

Lorenz

Anand

2004

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

show abstract

“…The neuropeptides of the family are usually octa-, nona-or decapeptides with both termini blocked (and one 11mer with nonamidated C-terminus - Köllisch et al, 2000), and are synthesized and released from the corpora cardiaca (CC). Besides the mobilization of energetic reserves these hormones exert additional physiological actions such as the inhibition of protein synthesis (Carlisle & Loughton, 1979;Cusinato et al, 1991), the inhibition of mRNA synthesis (Kodrík & Goldsworthy, 1995), the modulation of neuro-or myoactivity (Scarborough et al, 1984;Milde et al, 1995;Socha et al, 1999), and the inhibition of glycolysis (Becker & Wegener, 1998) and lipid synthesis (Gokuldas et al, 1988;Ziegler, 1997;Lorenz, 2001). Moreover, the adipokinetic hormone of Locusta migratoria (L.), Locmi-AKH-I, has been shown to increase the immune response in locusts challenged with laminarin or bacterial lipopolysaccharide (Goldsworthy et al, 2002a(Goldsworthy et al, , 2003.…”

Section: Introductionmentioning

confidence: 99%

The kind of AKH-mobilized energy substrates in insects can be predicted without a knowledge of the hormone structure

Socha¹,

Kodrı́k²,

Šimek³

et al. 2004

Eur. J. Entomol.

View full text Add to dashboard Cite

Abstract. The aim of this study was to show that the kind of AKH-mobilized energy substrates in insects can be predicted on the basis of the results obtained with the application of heterologous, i.e. inter-species, AKHs. Four different AKHs, the Locmi-AKH-I inducing hyperlipaemia and hyperglycaemia in Locusta migratoria, Tenmo-HrTH inducing hyperglycaemia in Tenebrio molitor, and Pyrap-AKH and Peram-CAH-II inducing hyperlipaemia in Pyrrhocoris apterus were used, firstly in conspecific tests, secondly in all possible species-AKH combinations, and finally in individual applications on the test species, the cotton bug Dysdercus cingulatus. Since each of the AKHs induced hyperlipaemia in D. cingulatus adults, we predicted that lipids are the only energy substrates which are mobilized in this species by its native AKH. The accuracy of this prediction was subsequently confirmed by the structural identification of the native D. cingulatus AKH and conspecific application tests. The proposed methodical approach can serve as a suitable monitoring system for determination of the kind of energy substrates mobilized by native insect AKHs until the structure of the hormone is identified.

show abstract

“…The inhibiting action of AKH on the synthesis of lipids is likely to be a general physiological effect in insects. This effect has been shown in S. gregaria (Gokuldas et al, 1988), in L. migratoria (Lee and Goldsworthy, 1995a,b), in G. morsitans, where CC-extracts have been tested (Pimley and Langley, 1981), in A. aegypti (Ziegler, 1997), and now in G. bimaculatus (this study).…”

Section: Discussionmentioning

confidence: 54%

Synthesis of lipids in the fat body of Gryllus bimaculatus: Age‐dependency and regulation by adipokinetic hormone

Lorenz

2001

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The free abdominal fat body of adult female Mediterranean field crickets, Gryllus bimaculatus, synthesizes lipids from [1-(14)C]-acetate in vitro. Up to an age of 12h, the incorporation of acetate into lipids is very low and then rises to a maximum 24h after adult emergence. Thereafter, the incorporation of acetate decreases to moderate levels at day 2 and then slowly decreases until day 30. The adipokinetic hormone of G. bimaculatus (Grb-AKH) significantly inhibits the incorporation of acetate at a concentration of 10(-11) M; maximum inhibition (approximately 95%) is reached at 10(-8) M. The inhibiting effect of Grb-AKH is fast, dose-dependent, and reversible. The periovaric fat body shows a similar pattern of acetate incorporation, although rates of incorporation are lower; the incorporation can be inhibited by Grb-AKH as well. The segmental abdominal fat body and the fat body from the head both incorporate acetate into lipids at low rates that cannot be inhibited significantly by AKH. Prepurified brain extracts significantly inhibit acetate incorporation by free abdominal fat bodies at a concentration of 0.1 brain equivalent. Allatostatins and crustacean cardioactive peptide, which are both present in cricket brains, are not responsible for this inhibiting effect. Octopamine causes a dose-dependent inhibition of acetate incorporation whereas synephrine had no such effect. The inhibiting effect of Grb-AKH on the formation of lipid stores in the fat body and its consequences for reproductive processes are discussed.

show abstract

The inhibition of lipid synthesis in vitro in the locust, Schistocerca gregaria, by factors from the corpora cardiaca

Cited by 56 publications

References 22 publications

Changes in the biochemical composition of fat body stores during adult development of female crickets, Gryllus bimaculatus

Changes in the biochemical composition of fat body stores during adult development of female crickets, Gryllus bimaculatus

The kind of AKH-mobilized energy substrates in insects can be predicted without a knowledge of the hormone structure

Synthesis of lipids in the fat body of Gryllus bimaculatus: Age‐dependency and regulation by adipokinetic hormone

Contact Info

Product

Resources

About