The inhibition of methicillin-resistant Staphylococcus aureus by essential oils isolated from leaves and fruits of Schinus areira depending on their chemical compositions.

Abstract

Schinus areira L. is a native plant from South America used for centuries in traditional medicine. Here, we investigate the antimicrobial activity of four essential oils extracted from leaves and fruits of S. areira exhibiting different chemical profiles. The antibacterial activity against the human pathogenic bacteria Staphylococcus aureus susceptible as well as methicillin resistant strain was assessed by the broth microdilution assay. The results showed that the limonene-rich oil extracted from the leaves and fruits have potent antibacterial effect on S. aureus ATCC 25923, while the α-phellandrene-rich fruit oil having a lower content of limonene showed the lowest antibacterial efficacy. In this work, for the first time, we demonstrated the bactericidal activity of essential oils isolated from fruits and leaves of S. areira against susceptible and methicillin resistant S. aureus strains. All results point out the potential use of the S. areira oils as antimicrobial agents to be used, at least against Staphylococcal infections.

Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

“…The Complex Activity Detection Kit was used to determine the effect of limonene on the respiratory chain complexes I~V of L. monocytogenes. The specific steps were, as follows: first, 5 × 10 6 bacterial cells of the control group (sterile water) and the experimental group (1 MIC) cultured for 4,8,12,16,20, and 24 h were collected; then, 1 mL of lysis buffer was added, and the cells were homogenized on ice with a homogenizer. The homogenate was centrifuged (4 • C, 600 g, 10 min.…”

“…Moreover, D-limonene can significantly inhibit gram-negative and gram-positive bacteria as well as fungal activity [11]. In addition, many researchers have confirmed that D-limonene can effectively inhibit the growth of spoilage bacteria, such as Aspergillus niger, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli [12,13]. Limonene, which is the main ingredient of lemon essential oil, was found to have antimicrobial activities against L. monocytogenes in minced beef meat [14].…”

Natural antioxidants isolated from Schinus areira leaves by ultrasound-assisted extraction

“…This study also demonstrated that clinical status (morbidity or mortality), macroscopic morphology and lung/body weight index were unaffected by the administration of the oils. We previously demonstrated that S. areira essential oils isolated from leaves and fruits have antibacterial activity (Celaya, Alabrudzinska, Molina, Viturro, & Moreno, 2014). Essential oils of a related specie, Schinus molle, also exhibited antioxidant and antimicrobial properties (Martins et al, 2014).…”

Chemical Composition and Biological Prospects of Essential Oils and Extracts of Aphyllocladus spartioides Growing in Northwest Argentina

“…EO was extracted from two samples of approximately 500 g of dried plant material by steam distillation for 2.5 h using a Clevenger ‐type apparatus. The collected oil samples were dried (Na 2 SO 4 ) and stored at 4 °C until analysis . The EO content was determined volumetrically on a dried weight basis, obtaining approximately the same yield.…”

“…EO composition was analyzed by GC‐IT‐MS and GC‐FID using a previously described procedure . GC‐IT‐MS analysis was carried out on a Hewlett Packard GC 6890 / MDS 5972 apparatus equipped with a HP‐5 MS Agilent Technologies column (30 m × 0.25 mm; 0.25 μm) and a Satum GC/MS workstation software version 6.8.…”