The inhibition of oxalate biosynthesis in isolated perfused rat liver by dl-phenyllactate and n-heptanoate

Liao, Lon Lon; Richardson, K.E.

doi:10.1016/0003-9861(73)90035-0

Cited by 51 publications

(8 citation statements)

References 26 publications

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“…One unit of GAD is defined as 1 p mol of 14C-oxalate produced/h at 3TC. Lactate dehydrogenase (LDH) was spectrophotometrically assayed by the method of Liao and Richardson [9] with glyoxylate used as substrate. The reaction mixture consisted of 0.5 M glycine buffer (pH 10.0), 0.1 ml of enzyme source, 20 p mol of NAD, and 501u mol sodium glyoxylate in a total volume of 2 ml.…”

Section: Methodsmentioning

confidence: 99%

Effect of Vitamin A Deficiency on the Specific Activities of Oxalate-Synthesizing Enzymes.

Kancha

Anasuya

1991

J. Clin. Biochem. Nutr.

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SummaryTo investigate the cause of hyperoxaluria in vitamin A deficiency, we studied the specific activities of the major oxalatesynthesizing enzymes in the liver, viz., glycolic acid oxidase (GAO), glycolic acid dehydrogenase (GAD), and lactate dehydrogenase (LDH). Pair-fed control and experimental (vitamin A deficient) groups of rats were fed a high-calcium (9g/kg diet), calculogenic diet for 12 weeks. Vitamin A deficiency resulted in increased specific activities of GAO, GAD (p<0.001), and LDH (p<0.05) compared with those of pair-fed controls. Correction of vitamin A deficiency returned the enzyme activities to the levels observed in control animals. The possible biochemical mechanism of hyperoxaluria in vitamin A deficiency is discussed.

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Section: Methodsmentioning

confidence: 99%

Effect of Vitamin A Deficiency on the Specific Activities of Oxalate-Synthesizing Enzymes.

Kancha

Anasuya

1991

J. Clin. Biochem. Nutr.

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“…Ten grams of liver were homogenised in 30 ml of cold 0-25 M sucrose, pH 7-0 for 20 sec at full speed in a Sorvall Omniniix. After centrifugation at 500 g for 5 min, aliquots of the supernatant, in appropriate dilutions, were assayed for glycollate oxidase, lactate dehydrogenase (Liao and Richardson, 1973), catalase (McGroarty et al, 1974) and alanine aminotransferase (Bergmeyer, 1963). Protein was detennined by the method of Lowry et al (1951).…”

Section: Methodsmentioning

confidence: 99%

Oxalate Excretion in Rats Injected With Xylitol or Glycollate: Stimulation by Phenobarbitone Pre‐treatment

Bais

et al. 1979

Aust J Exp Biol Med

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Summary The hypothesis that the prior intake of barbiturates may predispose patients to form increased amounts of oxalate following the intravenous infusion of xylitol was investigated in the rat. Phenobarbitone pre‐treatment resulted in a 2–3 fold increase in urinary |14C| oxalate concentration following the intra‐peritoneal injection of |U‐14C| xylitol or |1‐14C| glycollate. The absence of any marked changes in urine volumes and creatinine excretion implied that this increase in urinary oxalate excretion was due to the enhanced synthesis of oxalate. The activities of key enzymes in hepatic oxalate synthesis, glycollate oxidase, lactate dehydrogenase, catalase and alanine aminotransferase were not altered by phenobarbitone pre‐treatment. It is suggested that the increased activity of the microsomal mixed function oxidases, following phenobarbitone treatment, may facilitate the oxidation of glycollate and possibly xylitol. This communication lends experimental support to the concept that the prior intake of drugs, such as barbiturates, may predispose patients to form increased amounts of oxalate.

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“…GAO was assayed as described by Murthy et al [15] and Sharma et al [20] and GAD by the method of Fry and Richardson [4] with the modifications as suggested earlier [15,20], while LDH activity was measured using glyoxylate as substrate according to the method of Liao and Richardson [10]. Protein in all the samples was determined according to the method of Lowry et al [11].…”

Section: Methodsmentioning

confidence: 99%

Effect of Sodium Glycolate and Sodium Pyruvate on Oxalic Acid Biosynthesizing Enzymes in Rat Liver and Kidney

Murthy¹,

Talwar²,

Thind³

et al. 1983

Ann Nutr Metab

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Sodium glycolate feeding (50 mg/l00 g body weight/day) to adult male rats for 7 days resulted in increased activities of glycolate oxidase in liver and lactate dehydrogenase in liver and kidney. However, the activity of glycolate dehydrogenase decreased both in liver and kidney. Treatment of sodium pyruvate (100 mg/100 g body weight/day) to the glycolate-fed rats resulted in lowered liver glycolate oxidase activity, and the glycolate dehydrogenase activity was further decreased as compared to glycolate-fed rats in both age groups. However, lactate dehydrogenase activity was not affected by pyruvate feeding in comparison to the glycolate-treated group. It is concluded that glycolate-induced oxalate biosynthesis in rats involves increased activity of liver glycolate oxidase, and pyruvate feeding inhibits glycolate oxidase, thereby decreasing oxalate biosynthesis.

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The inhibition of oxalate biosynthesis in isolated perfused rat liver by dl-phenyllactate and n-heptanoate

Cited by 51 publications

References 26 publications

Effect of Vitamin A Deficiency on the Specific Activities of Oxalate-Synthesizing Enzymes.

Effect of Vitamin A Deficiency on the Specific Activities of Oxalate-Synthesizing Enzymes.

Oxalate Excretion in Rats Injected With Xylitol or Glycollate: Stimulation by Phenobarbitone Pre‐treatment

Effect of Sodium Glycolate and Sodium Pyruvate on Oxalic Acid Biosynthesizing Enzymes in Rat Liver and Kidney

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