The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism

Carneiro, Ronaldo Leal; Alípio, Ana Carla Nascimento; Bisch, Paulo Mascarello; Azevedo, Sandra Maria Feliciano de Oliveira e

doi:10.1590/s1517-83822011000400042

Cited by 12 publications

(6 citation statements)

References 16 publications

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“…1, Table 2). The results were corroborated by data from Carneiro et al (2011), who showed that growth in Cylindrospermopsis was inhibited by a continued exposure to high Ca 2 ' (8 mM). A field survey was implemented by Ye et al (2010), who showed that Ca 2 ' concentration was about 57.73 mg/l in the northern rivers of the Taihu Basin where large Microcystis blooms often occurred.…”

Section: Discussionsupporting

confidence: 68%

Physiological and molecular responses to calcium supplementation inMicrocystis aeruginosa(Cyanobacteria)

Shi¹,

Wu²,

Song³

2013

New Zealand Journal of Marine and Freshwater Research

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Calcium is an important element in water bodies that plays a pivotal role in numerous biological processes; however, its effects on bloom-forming cyanobacteria have been studied only rarely. In the present study, the effects of calcium on Microcystis aeruginosa, a major bloom-forming cyanobacterium, were investigated. Significant decreases in growth, chlorophyll a and maximum electron transport rate were shown when calcium was absent, while similar reductions in these parameters were also found at high concentrations of calcium (240 mg/l). The expression of psbA, grpE and recA was up-regulated significantly for 0 and 240 mg/l calcium treatments. The activity of catalase (CAT) also increased remarkably under these two treatments, suggesting that Microcystis suffered from stress under both calcium absence and under high calcium concentrations. Additionally, the polysaccharide synthesis gene, epsL, was up-regulated at high concentrations of calcium. These data suggest that calcium plays an important role in the growth of Microcystis, and that enhancement in polysaccharide synthesis in response to increases in calcium concentration might be responsible for bloom formation.

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Section: Discussionsupporting

confidence: 68%

Physiological and molecular responses to calcium supplementation inMicrocystis aeruginosa(Cyanobacteria)

Shi¹,

Wu²,

Song³

2013

New Zealand Journal of Marine and Freshwater Research

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“…Previously, an inhibitory effect of calcium chloride on C. raciborskii (T3) metabolism was reported [37]. Studying C. raciborskii saxitoxin biosynthesis in vitro , Kellman and Neilan [38] revealed that Ca 2+ was inhibitory.…”

Section: Resultsmentioning

confidence: 99%

Growth and Saxitoxin Production by Cylindrospermopsis raciborskii (Cyanobacteria) Correlate with Water Hardness

2013

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The cosmopolitan and increasing distribution of Cylindrospermopsis raciborskii can be attributed to its ecophysiological plasticity and tolerance to changing environmental factors in water bodies. In reservoirs in the semi-arid region of Brazil, the presence and common dominance of C. raciborskii have been described in waters that are considered hard. We investigated the response of a Brazilian C. raciborskii strain to water hardness by evaluating its growth and saxitoxin production. Based on environmental data, a concentration of 5 mM of different carbonate salts was tested. These conditions affected growth either positively (MgCO3) or negatively (CaCO3 and Na2CO3). As a control for the addition of cations, MgCl2, CaCl2 and NaCl were tested at 5 or 10 mM, and MgCl2 stimulated growth, NaCl slowed but sustained growth, and CaCl2 inhibited growth. Most of the tested treatments increased the saxitoxin (STX) cell quota after six days of exposure. After 12 days, STX production returned to concentrations similar to that of the control, indicating an adaptation to the altered water conditions. In the short term, cell exposure to most of the tested conditions favored STX production over neoSTX production. These results support the noted plasticity of C. raciborskii and highlight its potential to thrive in hard waters. Additionally, the observed relationship between saxitoxin production and water ion concentrations characteristic of the natural environments can be important for understanding toxin content variation in other harmful algae that produce STX.

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“…It also is linked integrally to river flow, and generally increases during periods of low flow because of lack of dilution from water with lower ionic content, a trend also evident in our data set (data not shown). Many of the major ions contributing to conductivity, e.g., Ca 21 , Cl 2 , Na 1 , K 1 , and Mg 21 , influence important metabolic processes or enhance/suppress growth in cyanobacteria (Seale et al 1987, Parker et al 1997, Carneiro et al 2011. Individual ions/compounds that contribute to conductivity may play a role in regulating Phormidium accrual, but further research is required to test this suggestion.…”

Section: Drivers Of % Phormidium Proliferationsmentioning

confidence: 99%

Effect of river flow, temperature, and water chemistry on proliferations of the benthic anatoxin-producing cyanobacterium Phormidium

et al. 2017

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Proliferations of the benthic anatoxin-producing cyanobacterium Phormidium are increasing in prevalence in cobble-bed rivers worldwide. This proliferation is of particular concern when rivers are used as sources of drinking water or for recreation. Little is known about the physicochemical variables promoting proliferations, and our existing knowledge is based on data from only a few rivers. We assessed Phormidium cover, physicochemical variables, and anatoxin concentrations at 10 sites in 7 New Zealand rivers every week for 2 y. Generalized additive mixed models (GAMMs) identified dissolved inorganic N (DIN) over the accrual period <0.8 mg/L, dissolved reactive P accrual <0.005 mg/L, water temperatures >157C, and conductivity as having positive and statistically significant effects on % Phormidium cover. Flow intensity, expressed relative to the long-term median, had a positive effect up to 0.4Â the median flow and a negative effect when >0.5Â the median flow. Quantile regression models showed marked variability among sites in relation to the flow intensity required to reduce % Phormidium cover (90 th percentile ranged 0.65-249Â the long-term median flow). Anatoxins were detected in variable concentrations in samples from 7 of the 10 sites. GAMMs identified strong relationships between elevated toxin concentrations and low conductivity and increasing % Phormidium cover, and significantly lower toxin concentrations when DIN was <0.2 mg/L. These data demonstrate that multiple physicochemical variables influence Phormidium proliferations and toxin concentrations and indicate that the relative importance of these variables differs among rivers and sites.

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The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism

Cited by 12 publications

References 16 publications

Physiological and molecular responses to calcium supplementation inMicrocystis aeruginosa(Cyanobacteria)

Physiological and molecular responses to calcium supplementation inMicrocystis aeruginosa(Cyanobacteria)

Growth and Saxitoxin Production by Cylindrospermopsis raciborskii (Cyanobacteria) Correlate with Water Hardness

Effect of river flow, temperature, and water chemistry on proliferations of the benthic anatoxin-producing cyanobacterium Phormidium

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