The inhibitory effect of environmental ammonia on Danio rerio LPS induced acute phase response

Gonçalves, Ana Clara Campagnolo Real; Páscoa, Inês; Neves, João V.; Coimbra, Joaquim Luís; Vijayan, Mathilakath M.; Rodrigues, Pedro; Wilson, Jonathan M.

doi:10.1016/j.dci.2011.04.008

Cited by 29 publications

(11 citation statements)

References 59 publications

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“…Thus, suppression of transcripts of the above-mentioned immune components in fish during transport could be attributed to the continued effect of cortisol resulting from various stressors including ammonia. The inhibitory effect of high ammonia on genes associated with innate immune response has been previously reported in zebrafish [13]. The suppression of transcripts of immune components during the transport may later on compromise the health of fish.…”

Section: Discussionmentioning

confidence: 76%

“…The complexity of changes in water quality in these transport units is dependent on the interrelationship between parameters such as ammonia, nitrite, nitrate, oxygen, carbon dioxide and pH [6], [7], [8], [9]. Ammonia accumulating in water during transport can be one of the main inducers of stress [10], [11], [12], [13]. In addition, oxygen super saturation can trigger oxidative stress [14], [15], [16] even though this parameter is not known to elevate cortisol or ammonia levels [17].…”

Section: Introductionmentioning

confidence: 99%

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Liver Transcriptome Changes in Zebrafish during Acclimation to Transport-Associated Stress

2013

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Liver plays a key role during the stress acclimation, and liver transcriptome analysis of shipped zebrafish could reveal the molecular adjustments that occur in the organ. Transcriptional changes in liver were analyzed with a 44 K oligo array using total RNA from fish prior to transport and during a mock transport process - immediately after packing (0 h), at 48 and 72 h. Large numbers of genes related to a variety of biological processes and pathways were regulated, mainly during transport (at 48/72 h). Immediately after packing, transcripts of genes related to both gluconeogenesis and glycolysis were induced. During transport, induction of gluconeogenesis-linked genes and reduction of glycolysis-related genes may be supporting the increase in blood glucose levels. Inhibition of genes involved in fatty acid beta-oxidation may be pointing to the poor ability of fish to utilize energy from fatty acids, under transport conditions. Genes involved in some of the mechanisms that regulate body ammonia were also affected. Even though genes associated with certain transaminases were inhibited in liver, sustained glutamate deamination may have led to high ammonia accumulation in liver/body. Enhanced levels of gene transcripts in ubiquitination and MAPK signalling cascade and reduced levels of gene transcripts related to ROS generation via peroxisomal enzymes as well as xenobiotic metabolism may be signifying the importance of such cellular and tissue responses to maintain homeostasis. Furthermore, transcripts connected with stress and thyroid hormones were also regulated. Moreover, suppression of genes related to specific immune components may be denoting the deleterious impact of transport on fish health. Thus, this study has revealed the complex molecular -adjustments that occur in zebrafish when they are transported.

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Liver Transcriptome Changes in Zebrafish during Acclimation to Transport-Associated Stress

2013

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“…Low concentration of ammonia-N has also been reported to decrease the virulence of white spot syndrome virus and Enterococcus [35,36]. Conversely, the high environmental ammonia was reported to display the inhibitory effect on LPS induced immune response in Danio rerio [37]. These results manifested that the concentration and exposure time of ammonia could influence the pathogen virulence in invertebrates.…”

Section: Time (D) Treatmentsmentioning

confidence: 69%

Alternation of immune parameters and cellular energy allocation of Chlamys farreri under ammonia-N exposure and Vibrio anguillarum challenge

Wang

Chen

et al. 2012

Fish & Shellfish Immunology

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“…This process was repeated for a total of three extractions and the ether was evaporated off overnight at room temperature. Cortisol quantification was performed with a commercially available ELISA (Neogen Corp, Lexington, KY) which has been used and characterized previously 8 40 , according to the manufacturer’s protocols. Steroid samples were reconstituted in extraction buffer from the ELISA kit.…”

Section: Methodsmentioning

confidence: 99%

Maternal Cortisol Mediates Hypothalamus-Pituitary-Interrenal Axis Development in Zebrafish

Nesan

Vijayan

2016

Sci Rep

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In zebrafish (Danio rerio), de novo synthesis of cortisol in response to stressor exposure commences only after hatch. Maternally deposited cortisol is present during embryogenesis, but a role for this steroid in early development is unclear. We tested the hypothesis that maternal cortisol is essential for the proper development of hypothalamus-pituitary-interrenal (HPI) axis activity and the onset of the stressor-induced cortisol response in larval zebrafish. In this study, zygotic cortisol content was manipulated by microinjecting antibody to sequester this steroid, thereby making it unavailable during embryogenesis. This was compared with embryos containing excess cortisol by microinjection of exogenous steroid. The resulting larval phenotypes revealed distinct treatment effects, including deformed mesoderm structures when maternal cortisol was unavailable and cardiac edema after excess cortisol. Maternal cortisol unavailability heightened the cortisol stress response in post-hatch larvae, whereas excess cortisol abolished the stressor-mediated cortisol elevation. This contrasting hormonal response corresponded with altered expression of key HPI axis genes, including crf, 11B hydroxylase, pomca, and star, which were upregulated in response to reduced cortisol availability and downregulated when embryos had excess cortisol. These findings for the first time underscore a critical role for maternally deposited cortisol in programming HPI axis development and function in zebrafish.

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The inhibitory effect of environmental ammonia on Danio rerio LPS induced acute phase response

Cited by 29 publications

References 59 publications

Liver Transcriptome Changes in Zebrafish during Acclimation to Transport-Associated Stress

Liver Transcriptome Changes in Zebrafish during Acclimation to Transport-Associated Stress

Alternation of immune parameters and cellular energy allocation of Chlamys farreri under ammonia-N exposure and Vibrio anguillarum challenge

Maternal Cortisol Mediates Hypothalamus-Pituitary-Interrenal Axis Development in Zebrafish

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