The Inhibitory Effect of Epigallocatechin Gallate on the Viability of T Lymphoblastic Leukemia Cells is Associated with Increase of Caspase-3 Level and Fas Expression

Ghasemi-Pirbaluti, Masome; Pourgheysari, Batoul; Shirzad, Hedayatollah; Sourani, Zahra; Beshkar, Pezhman

doi:10.1007/s12288-017-0854-4

Cited by 15 publications

(8 citation statements)

References 34 publications

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“…As the gene expression may not necessarily result in protein expression, the surface Fas was also studied. Such effects have already been reported by other plants-derived component in lymphoblastic leukemia cells (32). Pterostilbene also induces apoptosis through the caspase-independent pathway in both sensitive and chemo-resistant lymphoid leukemia cell lines (33).…”

Section: Discussionsupporting

confidence: 73%

Pterostilbene increases Fas expression in T-lymphoblastic leukemia cell lines

et al. 2019

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Treatment of acute lymphoblastic leukemia (ALL) has been promising in last decades, but side effects still persist and searching for the least toxic agents continue. Pterostilbene (PTE) is a natural compound with several anti-cancer and anti-oxidant properties. Fas, as a member of death inducing family of tumor necrosis factor (TNF) receptors with an intracellular death domain, can initiate the extrinsic apoptosis signaling pathway. Here after the half maximal inhibitory concentration (IC 50 ) determination in cell lines, we searched for PTE effects on Fas, both in mRNA and surface levels in two ALL cell lines, Jurkat and Molt-4. After harvesting cells in optimum situations, MTS assay was used to determine IC 50 concentrations. Real-time polymerase chain reaction (RT-PCR) and flow cytometry were performed for Fas mRNA and surface expression variations after exposure to PTE. The findings showed that PTE decreases cell viability with different extent in two ALL cell lines. In addition to inducing apoptosis, it can increase Fas in both gene and cell surface expression in the same concentrations. Pterostilbene as a natural anti-cancer agent can increase Fas expression both in mRNA and surface levels that results in apoptosis signal transduction improvement which sensitizes cells to apoptosis by immune effector cells. As a result, abnormal cells removal would be more efficiently with the minimum side effects on normal cells.

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Section: Discussionsupporting

confidence: 73%

Pterostilbene increases Fas expression in T-lymphoblastic leukemia cell lines

et al. 2019

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“…Cornwall Cull, et al have indicated that EGCG results in apoptosis in B-CLL and T-CLL cells in a dose-dependent manner, while it does not affect normal B and T cells [22]. Furthermore, it has been observed that EGCG induces apoptosis in Jurkat cell line through the expression of Fas and increasing Caspase 3 levels [23]. It has been also shown that EGCG prompts apoptosis in leukemic cells such as KG-1, TH-P1, and PML/RARα leukemic mice [24,25].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Epigallocatechin gallate on Cross-talk Between Autophagy and Apoptosis in NALM-6 Cell Line

Gharehchahi

Zare

Dehbidi

et al. 2021

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Background: Acute lymphoblastic leukemia is a prevalent hematological malignancy in 2-5-year-old children. Chemotherapy, as the most common treatment for ALL, is not usually responsive. Epigallocatechin gallate (EGCG), a small molecule extracted from green tea, has significant effects on tumor cells through different mechanisms, such as DNA damage, cell cycle arrest, oxidative stress, apoptosis, and autophagy. In this study, we investigated the impact of EGCG on autophagy and apoptosis in NALM-6 cell line. Methods and results: Cell viability and apoptosis were assessed by MTT and Trypan blue exclusion assay, and flow cytometry. It was shown that EGCG remarkably inhibited proliferation, reduced cell viability, and induced apoptosis in NALM-6 cell line (P<0.05). In addition, real-time PCR and western blot analysis were used to examine autophagy. It was observed that EGCG resulted in a 4-fold increase in LC3 protein level (P<0.05) while reducing the mRNA expression level of LC3B, P62/SQSTM1, and Atg2B genes (P<0.01). It also caused around 1.3-fold increase in DRAM1 mRNA expression level (P<0.05). Finally, it was indicated that the inhibition of autophagy affects apoptosis neither in untreated nor treated cells with EGCG.Conclusion: These results show that EGCG can induce apoptosis and autophagy in NALM-6 cell line while inhibition of autophagy cannot affect apoptosis in this cell line.

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“…In another study, on ALL Jurkat E6.1 cells, apoptosis was also present. Concentrations of EGCG that were used (50M, 70M, 100M) induced the apoptosis of 31%, 40%, and 71% of cells, respectively [17]. However, a chemically modified derivative of EGCG (MST-312) has been found effective as a telomerase inhibitor.…”

Section: Polyphenolsmentioning

confidence: 99%

Telomerase Inhibition Potential of Natural Compounds in Leukemia Treatment

Bartoszewska,

Molik,

Choromańska

et al. 2024

Preprint

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Leukemia encompasses diverse blood cancers marked by the unchecked growth and developmental blockage of myeloid or lymphoid precursor cells within the bone marrow and the bloodstream. Treatment traditionally involves intensive chemotherapy or hematopoietic stem cell transplantation, albeit these approaches often yield severe side effects and encounter resistance from leukemia cells. Hence, the quest for novel therapies targeting leukemic cells selectively is paramount to enhancing leukemia outcomes. Exploring natural products is a promising avenue for pursuing effective chemotherapy and preventive measures against leukemia. These compounds serve as a crucial wellspring for drug development and offer a source of biologically active elements for potential therapeutic interventions in leukemia. The enzyme telomerase plays a vital role in stabilizing chromosomes by adding DNA sections to the end of chromosomes during the mitotic cycle. Notably, hTERT mRNA expression strongly correlates with telomerase activity, making it a potential target for therapeutic intervention without adverse systemic effects. Telomerase inhibition is a promising target in cancer therapy, especially in combination with natural ingredients, demonstrating less cytotoxicity than chemotherapies.

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The Inhibitory Effect of Epigallocatechin Gallate on the Viability of T Lymphoblastic Leukemia Cells is Associated with Increase of Caspase-3 Level and Fas Expression

Cited by 15 publications

References 34 publications

Pterostilbene increases Fas expression in T-lymphoblastic leukemia cell lines

Pterostilbene increases Fas expression in T-lymphoblastic leukemia cell lines

The Effect of Epigallocatechin gallate on Cross-talk Between Autophagy and Apoptosis in NALM-6 Cell Line

Telomerase Inhibition Potential of Natural Compounds in Leukemia Treatment

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