The inhibitory effect of the leukotriene receptor antagonist on leukotriene D4-induced MUC2/5AC gene expression and mucin secretion in human airway epithelial cells

Bai, Chang Hoon; Song, Si-Youn; Kim, Yong‐Dae

doi:10.1016/j.anl.2006.11.006

Cited by 15 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This does not necessary deny the significance of physiological role of MUC2 and LTD 4 . Bai et al [29] examined the effect of LTD4 on the MUC2 gene expression and mucin secretion in human pulmonary mucoepidermoid carcinoma cell line (NCI-H292). 20 mol/l LTD 4 induced an approximately 3-fold increase in MUC2 gene expression and an 80% increase in…”

Section: Discussionmentioning

confidence: 99%

Leukotriene D4 Upregulates MUC2 Gene Transcription in Human Epithelial Cells

et al. 2008

View full text Add to dashboard Cite

Background/Aims: Leukotriene (LT) D₄ has been shown to induce mucus secretion in the airways. Excessive mucus secretion characterizes airway inflammatory disease such as asthma, allergic rhinitis. However, little is known about the effect of LTD₄ on mucin gene expression. The aim of this study was to investigate the effect of LTD₄ on MUC2 gene expression in cultured epithelial cells (HM3-MUC2 cells). Methods: HM3-MUC2 cells were treated with LTD₄ for 2 or 6 h. Reporter gene assay was mainly used for analysis.MUC2 protein levels were measured using an enzyme-linked immunosorbent assay. Results: LTD₄ significantly increased MUC2 gene transcriptional activity in a dose-dependent manner. Pranlukast, which is a selective antagonist of CysLT₁ receptor, inhibited LTD₄-induced MUC2 gene transcriptional activity in a dose-dependent manner. LTD₄-induced MUC2 gene transcriptional activity was also suppressed by a G-protein inhibitor (pertussis toxin),a protein kinase C (PKC) inhibitor (bisindolylmaleimide), a mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK) inhibitor (PD98059), an extracellular signal regulated kinase-2 (ERK-2) inhibitor (AG126) and a nuclear factor ĸB (NF-ĸB) inhibitor. In addition, pranlukast inhibited LTD₄-induced NF-ĸB activity. Conclusion: These results suggest that LTD₄upregulates MUC2 gene transcription via a signaling pathway involving CysLT₁ receptor, G-protein, PKC, MEK, ERK and NF-ĸB.

show abstract

Section: Discussionmentioning

confidence: 99%

Leukotriene D4 Upregulates MUC2 Gene Transcription in Human Epithelial Cells

et al. 2008

View full text Add to dashboard Cite

show abstract

“…MK-886 binds a membranebound 5-LO-activating protein, thereby inhibiting the translocation and activation of 5-LO, LTC 4 , 45 and leukotriene D 4 . 46,47 Th ese suggest that 5-LO products play a role in sPLA 2 -induced mucus secretion.…”

Section: Secretion In Goblet-enriched Cellsmentioning

confidence: 99%

Secretory Phospholipases A 2 Are Secreted From Ciliated Cells and Increase Mucin and Eicosanoid Secretion From Goblet Cells

Tanabe

Shimokawaji

Kanoh

et al. 2015

Chest

View full text Add to dashboard Cite

BACKGROUND: Secretory phospholipases A 2 (sPLA 2 ) initiate the biosynthesis of eicosanoids, are increased in the airways of people with severe asthma, and induce mucin hypersecretion. We used IL-13-transformed, highly enriched goblet cells and differentiated (ciliary cellenriched) human bronchial epithelial cell culture to evaluate the relative contribution of ciliated and goblet cells to airway sPLA 2 generation and response. We wished to determine the primary source(s) of sPLA 2 and leukotrienes in human airway epithelial cells.

show abstract

“…On the other hand, cysteinylleukotrienes induced mucin release from submandibular glands acting through their own receptor. In the human airway epithelium, leukotriene receptor system is one of the mechanisms related to MUC2/5AC gene expression and mucin secretion [3]. Our results indicate that cysteinylleukotrienes are able to induce mucin secretion in submandibular glands and that they are involved in the increment in basal mucin release observed in rats with ligature.…”

Section: Discussionmentioning

confidence: 59%

“…Increased mucus is likely to be associated with inflammatory responses and epithelial diseases. Inflammatory mediators such as prostaglandins [2], leukotrienes [3], nitric oxide [4], and tumor necrosis factor-a [5], among others, are involved in mucin secretion.…”

Section: Introductionmentioning

confidence: 99%

Increased leukotriene concentration× in submandibular glands from rats with experimental periodontitis

et al. 2009

View full text Add to dashboard Cite

show abstract

The inhibitory effect of the leukotriene receptor antagonist on leukotriene D4-induced MUC2/5AC gene expression and mucin secretion in human airway epithelial cells

Cited by 15 publications

References 17 publications

Leukotriene D<sub>4 </sub>Upregulates <i>MUC2</i> Gene Transcription in Human Epithelial Cells

Leukotriene D<sub>4 </sub>Upregulates <i>MUC2</i> Gene Transcription in Human Epithelial Cells

Secretory Phospholipases A 2 Are Secreted From Ciliated Cells and Increase Mucin and Eicosanoid Secretion From Goblet Cells

Increased leukotriene concentration× in submandibular glands from rats with experimental periodontitis

Contact Info

Product

Resources

About