The Inhzbitory Effect of D-Penicillamine on Human Lymphocyte Cultures Stimulated by Phytohaemagglutinin, The Antagonistic Action of L-Cysteine and Synergistic Inhibition by Copper Sulphate

Room, G; Roffe, Lindsay; Maini, R N

doi:10.3109/03009747909108236

Cited by 13 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition D-penicillamine has been shown to inhibit mitogen-induced lymphocyte transformation. 16 17 All these authors found a concentration-dependent effect with doses similar to ours. This work indicates that another type of cell originating from the mesoderm is also sensitive to the action of D-penicillamine and confirms that the chondrocyte is a target cell for this drug.…”

Section: Discussionsupporting

confidence: 82%

Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes.

Jaffray¹,

Ronot²,

Adolphe³

et al. 1984

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

Correspondence to Dr M. Adolphe. Material and methodsOrigin and culture of chondrocytes. Articular cartilage was removed from the shoulder and knee joints of 1-to 2-month old rabbits, Fauve de Bourgogne. Chondrocytes were enzymatically released from cartilage slices by the technique of Green,12 which gives a pure population of chondrocytes. Isolated cells were then cultured in HAM F 12 medium with 10% fetal calf serum and penicillin-streptomycin added. The medium was changed every second day after cell attachment had occurred, and the cuiture was maintained at 37°C in an atmosphere of 5% CO2. After the cells reached the plateau growth phase they were detached with trypsin, and the subculture was used for the present study.Drug. D-penicillamine hydrochloride was purchased for Sigma. Drug solutions were prepared on the day of the experiment by dissolving D-penicillamine in HAM F 12 medium. Serial appropriate dilutions were made at room temperature.Cell growth. 35 mm plastic Petri dishes were seeded with 7 x 104 cells. After one day of culture the dishes received fresh medium containing D-penicillamine hydrochloride at the appropriate concentration. After 24, 48, and 72 hours' incubation cells were collected by trypsinisation and the growth of control and treated cells was measured by cell 333 on 11 May 2018 by guest. Protected by copyright.

show abstract

Section: Discussionsupporting

confidence: 82%

Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes.

Jaffray¹,

Ronot²,

Adolphe³

et al. 1984

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

show abstract

“…D-PA forms stable mixed disulphides with many sulphydryl compounds, including cysteine, which may cause cysteine deficiency effects on in vitro systems. This caused some confusing results at first, but it is now established that D-PA in fact has a dose-dependent inhibitory effect on lymphocyte activation by phytohemagglutinin (19). Addition of cysteine diminishes this effect whereas CuS04 enhances inhibition.…”

Section: Effects In Vitro and On Animal Modelsmentioning

confidence: 99%

Penicillamine in 1980

Wolheim¹

1981

Journal of Internal Medicine

View full text Add to dashboard Cite

“…In vitro studies of the action of D-pen demonstrated that in lymphocyte cultures from normal individuals the mitogenic effects of phytohemagglutinin and concanavalin A were inhibited at D-pen doses of b 100 pg/ml.14322, 24 Investigations of immunoglobulin synthesis by human peripheral blood lymphocytes stimulated with pokeweed mitogen in short-term culture showed that preincubation with D-pen (100 pg/ml) alone had little effect and that significant inhibition of immunoglobulin synthesis occurred only when copper sulfate (3 pg/ml) was also p r e~e n t . '~ Although this inhibition of nonspecific immunoglobulin synthesis may be the basis for some of the observed changes in patients with rheumatoid arthritis, it offers no explanation for the appearance (or enhancement) of AChR antibody in patients who develop myasthenia while being treated with D-pen.…”

mentioning

confidence: 99%

D‐penicillamine‐associated myasthenia gravis: Immunological and electrophysiological studies

et al. 1982

View full text Add to dashboard Cite

Immunological and electrophysiological features were investigated in a patient with rheumatoid arthritis who developed myasthenic symptoms after 10 months of treatment with D-penicillamine (D-pen). After an initial rise, acetylcholine receptor (AChR) antibodies gradually fell from 24.4 nmole alpha-bungarotoxin bound/liter to 1.8 nmole/liter following withdrawal of the drug, and this was associated with clinical and electrophysiological improvement. Immunoglobuline synthesis by peripheral blood lymphocytes cultured with pokeweed mitogen and D-pen was significantly increased at D-pen concentrations of 10-100 micrograms/ml in 2 patients with D-pen associated myasthenia gravis and 1 out of 3 normal individuals. Furthermore, lymphocytes from 1 myasthenic patient synthesized detectable AChR antibodies in vitro, and the amount of receptor antibody produced was increased in the presence of the drug. This effect was most marked at a concentration of 10 micrograms/ml which is thought to approximate the concentration in vivo. Our results confirm the in vivo findings of others that D-pen may have different effects on immunoglobulin production in different individuals and may also enhance the snythesis of at least 1 autoantibody, the AChR antibody.

show abstract

The Inhzbitory Effect of D-Penicillamine on Human Lymphocyte Cultures Stimulated by Phytohaemagglutinin, The Antagonistic Action of L-Cysteine and Synergistic Inhibition by Copper Sulphate

Cited by 13 publications

References 11 publications

Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes.

Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes.

Penicillamine in 1980

D‐penicillamine‐associated myasthenia gravis: Immunological and electrophysiological studies

Contact Info

Product

Resources

About