The initial single yeast cell adhesion on glass via optical trapping and Derjaguin–Landau–Verwey–Overbeek predictions

Castelain, M.; Pignon, Frédéric; Piau, Jean‐Michel; Magnin, Albert

doi:10.1063/1.2842078

Cited by 17 publications

(11 citation statements)

References 55 publications

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“…Adhesion was strongest on surfaces with hydrophilic and hydrophobic properties, lower on purely hydrophobic surfaces, and lowest on hydrophilic surfaces, regardless of FLO11 expression (58). The adhesion strengths to different surfaces were measured using AFM (59) and optical tweezers (60).…”

Section: Discussionmentioning

confidence: 99%

Single cell analysis reveals unexpected growth phenotype of S. cerevisiae

2008

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Single cell analysis is mainly limited to single time-point measurements, without the possibility to track behavioral changes of a single cell or its descendents. Here, the integration of a spatiotemporal single cell lab-on-a-chip system with an automated cultivation device allows single cell analysis under defined growth conditions and, especially, semiautomated cell retrieval and growth kinetic analysis of the single cell descendants. Performance of the new platform was evaluated using the yeast Saccharomyces cerevisiae. The yeast was singularized in the lab-on-a-chip (Envirostat), which combines the possibility of cell cultivation with cell analysis. Singularized cells were collected in a microtiter plate and cultivated in a semiautomated cultivation device (Bioscreen C). S. cerevisiae showed highly reproducible and glucose concentration independent growth kinetics in population experiments. Yet, growth kinetics in cultures inoculated with only one or few cells exhibited strong variations, because of an unexpected growth phenotype: colony formation in submerse cultures. Interestingly, the colony-like structures grew for more then 60 h and were stable for at least 82 h, despite rigorous shaking. Cell agglomeration due to pseudohyphal growth could be excluded, suggesting changes in cell wall properties of yeast populations starting from a single yeast. Single cell analysis still exhibits unexpected obstacles. Nevertheless, this new single cell analysis platform can be used for studying cellular dynamics of single cells and expanded cell populations thereof. '

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Section: Discussionmentioning

confidence: 99%

Single cell analysis reveals unexpected growth phenotype of S. cerevisiae

2008

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“…They gained information on the number, strength, and reaction rates of the bonds. Optical tweezers have also been applied to study the adhesion of Saccharomyces cerevisiae cells [ 134 , 135 , 138 ] and to map the adhesion force during the formation and maturation of cell adhesion sites of mouse embryonic fibroblasts [ 136 ].…”

Section: Types Of Adhesion Studiesmentioning

confidence: 99%

A Review of Cell Adhesion Studies for Biomedical and Biological Applications

Khalili

Ahmad

2015

IJMS

763

545

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Cell adhesion is essential in cell communication and regulation, and is of fundamental importance in the development and maintenance of tissues. The mechanical interactions between a cell and its extracellular matrix (ECM) can influence and control cell behavior and function. The essential function of cell adhesion has created tremendous interests in developing methods for measuring and studying cell adhesion properties. The study of cell adhesion could be categorized into cell adhesion attachment and detachment events. The study of cell adhesion has been widely explored via both events for many important purposes in cellular biology, biomedical, and engineering fields. Cell adhesion attachment and detachment events could be further grouped into the cell population and single cell approach. Various techniques to measure cell adhesion have been applied to many fields of study in order to gain understanding of cell signaling pathways, biomaterial studies for implantable sensors, artificial bone and tooth replacement, the development of tissue-on-a-chip and organ-on-a-chip in tissue engineering, the effects of biochemical treatments and environmental stimuli to the cell adhesion, the potential of drug treatments, cancer metastasis study, and the determination of the adhesion properties of normal and cancerous cells. This review discussed the overview of the available methods to study cell adhesion through attachment and detachment events.

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“…However, microbial adhesion has been dissected into several mechanisms that act either in parallel or in a consecutive manner. These include non-specific interactions, for which the description is strongly inspired by colloidal disciplines such as physicochemical approaches with DLVO theory [ 83 , 84 , 85 , 86 ] to the specific side, driven by the ligand-receptor mechanisms [ 14 , 87 , 88 ] including glycan-lectin interactions. In most cases, bacteria initially adhere to host cells through the action of adhesins that bind specifically to receptors at the surface of host cells.…”

Section: Mechanisms Of Glycan-based Adhesion Of Bacteriamentioning

confidence: 99%

The Role of Glycans in Bacterial Adhesion to Mucosal Surfaces: How Can Single-Molecule Techniques Advance Our Understanding?

et al. 2018

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Bacterial adhesion is currently the subject of increased interest from the research community, leading to fast progress in our understanding of this complex phenomenon. Resent research within this field has documented the important roles played by glycans for bacterial surface adhesion, either through interaction with lectins or with other glycans. In parallel with this increased interest for and understanding of bacterial adhesion, there has been a growth in the sophistication and use of sensitive force probes for single-molecule and single cell studies. In this review, we highlight how the sensitive force probes atomic force microscopy (AFM) and optical tweezers (OT) have contributed to clarifying the mechanisms underlying bacterial adhesion to glycosylated surfaces in general and mucosal surfaces in particular. We also describe research areas where these techniques have not yet been applied, but where their capabilities appear appropriate to advance our understanding.

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The initial single yeast cell adhesion on glass via optical trapping and Derjaguin–Landau–Verwey–Overbeek predictions

Cited by 17 publications

References 55 publications

Single cell analysis reveals unexpected growth phenotype of S. cerevisiae

Single cell analysis reveals unexpected growth phenotype of S. cerevisiae

A Review of Cell Adhesion Studies for Biomedical and Biological Applications

The Role of Glycans in Bacterial Adhesion to Mucosal Surfaces: How Can Single-Molecule Techniques Advance Our Understanding?

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