The Ins and Outs of Hematopoietic Stem Cells: Studies to Improve Transplantation Outcomes

Marquez‐Curtis, Leah A.; Turner, A. Robert; Sridharan, Santhi; Ratajczak, Mariusz Z.; Janowska‐Wieczorek, Anna

doi:10.1007/s12015-010-9212-8

Cited by 56 publications

(64 citation statements)

References 205 publications

(218 reference statements)

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“…In stem cell mobilization, granulocytes are activated by G-CSF application, chemotherapy, or irradiation, which convert the BM in a highly proteolytic environment [3,27,28]. The association of neutrophils and increasing elastase concentrations during mobilization of HPC, and the failure of HPC mobilization during neutropenia, suggest that neutrophils and neutrophil proteases are required for HPC mobilization [29,30]. A role for neutrophil elastase in HPC mobilization is also supported by experiments showing that the application of a peptidergic elastase inhibitor reduces G-CSF-induced HPC mobilization [31].…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Richter

Jochheim-Richter

Ciuculescu

et al. 2014

Stem Cells and Development

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Mobilization of hematopoietic stem and progenitor cells (HPCs) is induced by treatment with granulocytecolony stimulating factor, chemotherapy, or irradiation. We observed that these treatments are accompanied by a release of chemotactic activity into the blood. This plasma activity is derived from the bone marrow, liver, and spleen and acts on HPCs via the chemokine receptor CXCR4. A human blood peptide library was used to characterize CXCR4-activating compounds. We identified CXCL12 or CXCL12 induced a significant mobilization of HPCs in mice. Our findings indicate that plasma-derived CXCL12 variants may contribute to the regulation of HPC mobilization by modulating the binding of CXCL12 to GAGs rather than blocking the CXCR4 receptor and, therefore, may have a contributing role in HPC mobilization.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Richter

Jochheim-Richter

Ciuculescu

et al. 2014

Stem Cells and Development

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“…The reverse process during which HSCs cross the endothelial wall and the extracellular matrix barriers is similarly controlled (46,132,186,188,189,264,268,290,299,332,342,343). Knowledge of the molecular pathways governing the migration of HSCs has been translated to the clinic; innovative protocols for the harvest of large numbers of HSCs and the optimization of their engraftment after systemic infusion have been developed to enhance the success of bone marrow transplantation (5,121,150,219,220,268,287). In addition to HSCs, it has been recognized recently that the bone marrow serves as a reservoir for other types of stem and progenitors cells, including endothelial progenitor cells and mesenchymal stem cells (MSCs).…”

Section: Stem Cell Traffickingmentioning

confidence: 99%

Aging Effects on Cardiac Progenitor Cell Physiology

Rota

Goichberg

Anversa

et al. 2015

Comprehensive Physiology

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Cardiac aging has been confounded by the concept that the heart is a postmitotic organ characterized by a predetermined number of myocytes, which is established at birth and largely preserved throughout life until death of the organ and organism. Based on this premise, the age of cardiac cells should coincide with that of the organism; at any given time, the heart would be composed of a homogeneous population of myocytes of identical age. The discovery that stem cells reside in the heart and generate cardiac cell lineages has imposed a reconsideration of the mechanisms implicated in the manifestations of the aging myopathy. The progressive alterations of terminally differentiated myocytes, and vascular smooth muscle cells and endothelial cells may represent an epiphenomenon dictated by aging effects on cardiac progenitor cells (CPCs). Changes in the properties of CPCs with time may involve loss of self-renewing capacity, increased symmetric division with formation of daughter committed cells, partial depletion of the primitive pool, biased differentiation to the fibroblast fate, impaired ability to migrate, and forced entry into an irreversible quiescent state. Telomere shortening is a major variable of cellular senescence and organ aging, and support the notion that CPCs with critically shortened or dysfunctional telomeres contribute to myocardial aging and chronic heart failure. These defects constitute the critical variables that define the aging myopathy in humans. Importantly, a compartment of functionally competent human CPCs persists in the decompensated heart pointing to stem cell therapy as a novel form of treatment for the aging myopathy.

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“…Successful allogeneic HSC or BM transplantation relies heavily on the ability of the HLA-matched donor cells to replace the recipient's hematopoietic functions by homing and engrafting into the host BM niche (7)(8)(9). Currently, all transplantations require recipient conditioning, which involves cytoreductive agents and/or irradiation to improve donor cell engraftment (10)(11)(12).…”

mentioning

confidence: 99%

In vivo engineering of bone tissues with hematopoietic functions and mixed chimerism

Shih

Rao

Chiu

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Synthetic biomimetic matrices with osteoconductivity and osteoinductivity have been developed to regenerate bone tissues. However, whether such systems harbor donor marrow in vivo and support mixed chimerism remains unknown. We devised a strategy to engineer bone tissues with a functional bone marrow (BM) compartment in vivo by using a synthetic biomaterial with spatially differing cues. Specifically, we have developed a synthetic matrix recapitulating the dual-compartment structures by modular assembly of mineralized and nonmineralized macroporous structures. Our results show that these matrices incorporated with BM cells or BM flush transplanted into recipient mice matured into functional bone displaying the cardinal features of both skeletal and hematopoietic compartments similar to native bone tissue. The hematopoietic function of bone tissues was demonstrated by its support for a higher percentage of mixed chimerism compared with i.v. injection and donor hematopoietic cell mobilization in the circulation of nonirradiated recipients. Furthermore, hematopoietic cells sorted from the engineered bone tissues reconstituted the hematopoietic system when transplanted into lethally irradiated secondary recipients. Such engineered bone tissues could potentially be used as ectopic BM surrogates for treatment of nonmalignant BM diseases and as a tool to study hematopoiesis, donor–host cell dynamics, tumor tropism, and hematopoietic cell transplantation.

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The Ins and Outs of Hematopoietic Stem Cells: Studies to Improve Transplantation Outcomes

Cited by 56 publications

References 205 publications

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Identification and Characterization of Circulating Variants of CXCL12 from Human Plasma: Effects on Chemotaxis and Mobilization of Hematopoietic Stem and Progenitor Cells

Aging Effects on Cardiac Progenitor Cell Physiology

In vivo engineering of bone tissues with hematopoietic functions and mixed chimerism

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