The integration of NSC-derived and host neural networks after rat spinal cord transection

Lai, Bi-Qin; Wang, Junmei; Duan, Jingjing; Chen, Yong; Gu, Huaiyu; Ling, Eng‐Ang; Wu, Jin-Lang; Zeng, Yuan-Shan

doi:10.1016/j.biomaterials.2012.12.046

Cited by 41 publications

(35 citation statements)

References 44 publications

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“…The strategy of transplantation of in vitro prebuilt SCLT consisting of differentiated cells is believed to increase the safety of donor cells31 and dodge the uncertainty of cell differentiation amid postinjury microenvironment when compared to direct transplantation of stem or progenitor cells. [[qv: 10a,32]] Following SCLT transplantation, the overall hind limb motor function was significantly improved, compared with the control groups; in fact, it was superior to that of what we reported before using NSC‐derived neural network transplantation (equivalent to the GMLT transplantation) 12. Consistent with previous observation, the GMLT module of the SCLT was capable of making contacts with the host descending or ascending neural circuits that would potentially serve as “tissue engineering neuronal relay” to conduct signals passing through the injury/graft site of spinal cord.…”

Section: Discussionmentioning

confidence: 92%

“…To strengthen the safety, the stem cells were induced into young neurons in vitro, prior to transplantation 11. The preconstructed functional neuronal network had provided a favorable microenvironment that increased the survival of donor cells, thereby fostering the regeneration of the host nervous tissue 11, 12. More importantly, this in vitro tissue construction strategy is believed to minimize the uncertainty of stem cell differentiation in vivo.…”

Section: Introductionmentioning

confidence: 99%

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A Modular Assembly of Spinal Cord–Like Tissue Allows Targeted Tissue Repair in the Transected Spinal Cord

et al. 2018

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Tissue engineering–based neural construction holds promise in providing organoids with defined differentiation and therapeutic potentials. Here, a bioengineered transplantable spinal cord–like tissue (SCLT) is assembled in vitro by simulating the white matter and gray matter composition of the spinal cord using neural stem cell–based tissue engineering technique. Whether the organoid would execute targeted repair in injured spinal cord is evaluated. The integrated SCLT, assembled by white matter–like tissue (WMLT) module and gray matter–like tissue (GMLT) module, shares architectural, phenotypic, and functional similarities to the adult rat spinal cord. Organotypic coculturing with the dorsal root ganglion or muscle cells shows that the SCLT embraces spinal cord organogenesis potentials to establish connections with the targets, respectively. Transplantation of the SCLT into the transected spinal cord results in a significant motor function recovery of the paralyzed hind limbs in rats. Additionally, targeted spinal cord tissue repair is achieved by the modular design of SCLT, as evidenced by an increased remyelination in the WMLT area and an enlarged innervation in the GMLT area. More importantly, the pro‐regeneration milieu facilitates the formation of a neuronal relay by the donor neurons, allowing the conduction of descending and ascending neural inputs.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

A Modular Assembly of Spinal Cord–Like Tissue Allows Targeted Tissue Repair in the Transected Spinal Cord

et al. 2018

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“…Silk fibroin-chitosan (SF-CS) scaffolds have been studied for their applicability in many fields such as medicine, pharmacy, tissue engineering, and basic sciences 5,6) . SF-CS scaffolds not only provide a space for cells to organize into a three-dimensional (3D) structure, they also provide mechanical integrity and a hydrated space for the diffusion of nutrients and metabolites to and from the cells 7) . Silk fibroin (SF) and chitosan (CS) are two prominent biopolymers currently being studied for the fabrication of tissue scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Construction and <i>in vitro</i> characterization of three-dimensional silk fibroinchitosan scaffolds

Tong

Liu

et al. 2015

Dental Materials Journal

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The objective of this study was to discuss the construction method, characterization, and biocompatibility of three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met the requirements of bone tissue engineering scaffolds. Silk fibroin (SF) and chitosan (CS) were mixed at different ratios -3 to 7, 5 to 5, and 7 to 3-to fabricate the composite materials. To find out the optimum mixing ratio of SF and CS, parameters such as pore size, porosity, water absorption, and the mechanical properties were evaluated. Osteoblast cells hFOB1.19 were seeded on SF-CS scaffolds and pure CS scaffolds for the first time. Cell adhesion rate, cell proliferation, and cell activity were evaluated, and cell growth and formation of mineralized nodules were observed. Results showed that SF-CS scaffolds are a suitable candidate for bone tissue engineering.

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“…On the other hand, in a complete or more severe SCI, it has become clear that there is no single ''magic bullet'' that allows concurrent remyelination, neuronal survival, and axonal regeneration [8]. In this connection, the design of a tissueengineered neural network, with the core concept of tissue engineering consisting of cells, bioactive molecules, and scaffolds, as well as their mutual interactions seems promising [9,10]. With the optimal combination of three elements mentioned above, tissue engineering approach is expected to bridge the cavities as well as to promote the remyelination and regeneration of axons in the injured area.…”

mentioning

confidence: 99%

“…Sheet-like structures with large surface areas have been shown to provide a successful seal of adhered cells. The niches are believed to have profound effect on lineage specification of NSCs, which tend to differentiate into neural cells [10]. In SCI animal model, threedimensional (3D) GS appeared to provide guidance for regenerating axons, facilitate remyelination, and prevent the infiltration of scar tissue and cyst formation [10,15].…”

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confidence: 99%

Graft of a Tissue-Engineered Neural Scaffold Serves as a Promising Strategy to Restore Myelination after Rat Spinal Cord Transection

Lai

Wang²,

Ling³

et al. 2014

Stem Cells and Development

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Remyelination remains a challenging issue in spinal cord injury (SCI). In the present study, we cocultured Schwann cells (SCs) and neural stem cells (NSCs) with overexpression of neurotrophin-3 (NT-3) and its high affinity receptor tyrosine kinase receptor type 3 (TrkC), respectively, in a gelatin sponge (GS) scaffold. This was aimed to generate a tissue-engineered neural scaffold and to investigate whether it could enhance myelination after a complete T10 spinal cord transection in adult rats. Indeed, many NT-3 overexpressing SCs (NT-3-SCs) in the GS scaffold assumed the formation of myelin. More strikingly, a higher incidence of NSCs overexpressing TrkC differentiating toward myelinating cells was induced by NT-3-SCs. By transmission electron microscopy, the myelin sheath showed distinct multilayered lamellae formed by the seeded cells. Eighth week after the scaffold was transplanted, some myelin basic protein (MBP)-positive processes were observed within the transplantation area. Remarkably, certain segments of myelin derived from NSC-derived myelinating cells and NT-3-SCs were found to ensheath axons. In conclusion, we show here that transplantation of the GS scaffold promotes exogenous NSC-derived myelinating cells and SCs to form myelins in the injury/transplantation area of spinal cord. These findings thus provide a neurohistological basis for the future application or transplantation using GS neural scaffold to repair SCI.

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The integration of NSC-derived and host neural networks after rat spinal cord transection

Cited by 41 publications

References 44 publications

A Modular Assembly of Spinal Cord–Like Tissue Allows Targeted Tissue Repair in the Transected Spinal Cord

A Modular Assembly of Spinal Cord–Like Tissue Allows Targeted Tissue Repair in the Transected Spinal Cord

Construction and <i>in vitro</i> characterization of three-dimensional silk fibroinchitosan scaffolds

Graft of a Tissue-Engineered Neural Scaffold Serves as a Promising Strategy to Restore Myelination after Rat Spinal Cord Transection

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