The interaction of a structural flexible small molecule with nucleic acid structures: Investigation of the origin of fluorescence signal discrimination in sensing and the utilization in live cell imaging

A study on a telo21 G-quadruplex DNA specific binding ligand: enhancing the molecular recognition ability via the amino group interactions

“…All those interactions with G-quartets constrain the π-conjugation system of 1 to a more planar conformation as compared to its unbound form, which is conformational flexible due to the ethylene bridge. 22 The docking result explains the experimental observation that the ligand system showed weak fluorescent signal in aqueous or physiological conditions while exhibited strong fluorescent signal induction upon binding to telo21 G-quadruplex structure. Interestingly, our findings based on both experimental and docking results are in accord with to previously reported results that the introduction of a net positive charge onto the side chain (through the nitrogen atom of the terminal morpholino moiety) of a series of fluorenone carboxamides can improve G-quadruplex binding (docking study based on , a NMR optimized DNA G-quadruplex model GRID MIFs, related to the telomeric sequence d[AG3(T2AG3) 3 ]).…”

“…1 , L). 22 The reported ligand L shows only limited signal discrimination ability although its signaling specificity towards G4-DNA is merit. Probably due to the ligand only has one amine group for interaction.…”

Stabilization and fluorescence light-up of G-quadruplex nucleic acids using indolyl-quinolinium based probes

“…57 Sun and co-workers reported planar and rotatable π-conjugated indole and pyridinium molecules linked by an ethylene bridge to improve the structural flexibility of the conjugated two ring co-planar system, demonstrating remarkable fluorescence discrimination between G4 DNA and other DNA tertiary structures. Xie and co-workers 58 reported indole- or pyrrole-substituted distyryl and mono-styryl dyes and studied their fluorescent light-up properties for G4 DNAs, duplex DNA, or single-stranded RNA. 59 However, none of these probes were found to have a preference for a particular G4 topology.…”

Fluorescent Benzothiadiazole Derivatives as Fluorescence Imaging Dyes: A Decade of New Generation Probes

“…The nucleus is important, inter alia, for gene expression, cell division, growth, protein synthesis, which are all well documented. In this sense, several works are available describing the selective staining of this organelle and/or of its internal compartments [70–75] …”

“…In this sense, several works are available describing the selective staining of this organelle and/or of its internal compartments. [70][71][72][73][74][75] The first example a fluorogenic BTD applied as a cell imaging probe was described nearly ten years ago by our group. [20] In the work, fluorescent BTD-based dyes have been used to stain the dsDNA of human stem-cells selectively.…”