The interaction of chitosan and BMP‐2 tuned by deacetylation degree and pH value

Wang, Qun; Wang, Menghao; Li, Pengfei; Wang, Kefeng; Fang, Liming; Ren, Fuzeng; Lu, Guoming; Li, Xiong

doi:10.1002/jbm.a.36593

Cited by 20 publications

(14 citation statements)

References 44 publications

(70 reference statements)

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“…Although both BMP‐2 and CS are positively charged, molecular dynamics simulations by Wang et al showed an attractive interaction of these two substances, which depends on the degree of deacetylation of CS (Wang et al, 2019). Experimentally, a loading of CS particles with BMP‐2 could only be shown for sulfated CS so far (Cao, Wang, Hou, Xing, & Liu, 2014), which is to be expected because of the known interaction of BMP‐2 with sulfated glycosaminoglycans such as heparin.…”

Section: Discussionmentioning

confidence: 99%

Varying the sustained release of BMP‐2 from chitosan nanogel‐functionalized polycaprolactone fiber mats by different polycaprolactone surface modifications

Sundermann

Oehmichen

Sydow

et al. 2020

J Biomedical Materials Res

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Polycaprolactone (PCL) fiber mats with different surface modifications were functionalized with a chitosan nanogel coating to attach the growth factor human bone morphogenetic protein 2 (BMP‐2). Three different hydrophilic surface modifications were compared with regard to the binding and in vitro release of BMP‐2. The type of surface modification and the specific surface area derived from the fiber thickness had an important influence on the degree of protein loading. Coating the PCL fibers with polydopamine resulted in the binding of the largest BMP‐2 quantity per surface area. However, most of the binding was irreversible over the investigated period of time, causing a low release in vitro. PCL fiber mats with a chitosan‐graft‐PCL coating and an additional alginate layer, as well as PCL fiber mats with an air plasma surface modification boundless BMP‐2, but the immobilized protein could almost completely be released. With polydopamine and plasma modifications as well as with unmodified PCL, high amounts of BMP‐2 could also be attached directly to the surface. Integration of BMP‐2 into the chitosan nanogel functionalization considerably increased binding on all hydrophilized surfaces and resulted in a sustained release with an initial burst release of BMP‐2 without detectable loss of bioactivity in vitro.

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Section: Discussionmentioning

confidence: 99%

Varying the sustained release of BMP‐2 from chitosan nanogel‐functionalized polycaprolactone fiber mats by different polycaprolactone surface modifications

Sundermann

Oehmichen

Sydow

et al. 2020

J Biomedical Materials Res

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“…Chitosan is known to bind BMP-2 although both molecules have an overall positive charge at neutral pH [27]. The chitosan binding may have a solubilizing effect on BMP-2 by preventing its ionic self-interaction (Figure 9).…”

Section: Discussionmentioning

confidence: 99%

“…Left (without chitosan): Ionic BMP-2 self-interaction, leading to BMP-2 aggregation [23]. Right: Binding of chitosan to the negatively charged tips of BMP-2 [27], potentially leading to a decreased aggregation tendency.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, chitosan, which may be applied in controlled release systems for BMP-2 [25,26], was investigated as possible interfering substance. Although both, BMP-2 and chitosan, are positively charged at physiological pH, molecular dynamics simulations revealed an attractive interaction of these two substances [27]. Taking the two potential effects together, we wanted to explore whether the BMP-2 release as determined in in vitro studies was influenced by aggregation-correlated recovery effects in PBS-based buffers and SBF, especially in the presence of chitosan.…”

Section: Introductionmentioning

confidence: 99%

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ELISA- and Activity Assay-Based Quantification of BMP-2 Released In Vitro Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan

et al. 2021

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Chitosan nanogel-coated polycaprolactone (PCL) fiber mat-based implant prototypes with tailored release of bone morphogenic protein 2 (BMP-2) are a promising approach to achieve implant-mediated bone regeneration. In order to ensure reliable in vitro release results, the robustness of a commercially available ELISA for E. coli-derived BMP-2 and the parallel determination of BMP-2 recovery using a quantitative biological activity assay were investigated within a common release setup, with special reference to solubility and matrix effects. Without bovine serum albumin and Tween 20 as solubilizing additives to release media buffed at physiological pH, BMP-2 recoveries after release were notably reduced. In contrast, the addition of chitosan to release samples caused an excessive recovery. A possible explanation for these effects is the reversible aggregation tendency of BMP-2, which might be influenced by an interaction with chitosan. The interfering effects highlighted in this study are of great importance for bio-assay-based BMP-2 quantification, especially in the context of pharmaceutical release experiments.

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“…Chitosan, a biopolymer derived from chitin, has many useful properties such as biocompatibility, biodegradability, and antimicrobial properties, and has been extensively studied for applications in tissue engineering (Wang et al, 2019). PVA is a water-soluble, biodegradable and non-toxic polymer.…”

Section: Discussionmentioning

confidence: 99%

Self‐assembling hydrogel loaded with 5‐FU PLGA microspheres as a novel vitreous substitute for proliferative vitreoretinopathy

et al. 2020

J Biomedical Materials Res

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The vitreous substitute for proliferative vitreoretinopathy (PVR) surgery remains an unmet clinical need in ophthalmology. In our study, we developed an in situ formed hydrogel by crosslinking polyvinyl alcohol (PVA) and chitosan as a potential vitreous substitute. 5-fluorouracil (5-FU) Poly (lactic-co-glycolic acid) (PLGA) microspheres were developed and loaded onto the PVA/chitosan hydrogels to treat PVR. In vitro, PVA/chitosan hydrogels at four concentrations were subjected to morphological, physical, rheological analyses, and cytotoxicity was evaluated together with the characterization of 5-FU PLGA microspheres. In vivo, pharmacologically induce PVR rabbits were performed a vitrectomy. In the PVA group, 3% PVA/chitosan hydrogel was injected into the vitreous cavity. In the PVA/MS group, 3% PVA/chitosan hydrogel and 5-FU PLGA microspheres were injected. In the Control group, phosphate-buffered saline was injected. Therapeutic efficacy was evaluated with postoperative examinations and histological analyses. This study demonstrated that the 3% PVA/chitosan hydrogel showed properties similar to those of the human vitreous and could be a novel in situ crosslinked vitreous substitute for PVR. Loading 5-FU PLGA microspheres onto this hydrogel may represent an effective strategy to improve the prognosis of PVR.

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The interaction of chitosan and BMP‐2 tuned by deacetylation degree and pH value

Cited by 20 publications

References 44 publications

Varying the sustained release of BMP‐2 from chitosan nanogel‐functionalized polycaprolactone fiber mats by different polycaprolactone surface modifications

Varying the sustained release of BMP‐2 from chitosan nanogel‐functionalized polycaprolactone fiber mats by different polycaprolactone surface modifications

ELISA- and Activity Assay-Based Quantification of BMP-2 Released In Vitro Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan

Self‐assembling hydrogel loaded with 5‐FU PLGA microspheres as a novel vitreous substitute for proliferative vitreoretinopathy

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