The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver

Ostuni, Emanuele; Yan, Lin; Whitesides, George M.

doi:10.1016/s0927-7765(99)00004-1

Cited by 447 publications

(374 citation statements)

References 167 publications

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“…Protein adsorption on a material is highly dependent upon the material's surface free energy and functionality (16,17,18). Numerous studies have been conducted to passivate material surfaces to cell and protein adhesion using simple hydrophobic and hydrophilic polymers and thin films.…”

Section: Introductionmentioning

confidence: 99%

Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Follstaedt¹,

Cheung²,

Gourley³

et al. 2000

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The interface of biology and semiconductor materials has become an important topic of interest as researchers are merging living organisms with microelectromechanical systems (MEMS) in the pursuit of new microfabricated biomedical devices. Biological cells interface with the semiconductor surface through a host of interactions that are mediated by protein adhesion and film formation. To better understand this interface, we have conducted protein adsorption studies of a model system using bovine serum albumin (BSA) and compared it to the adsorption from a complex protein mixture of cell growth serum on uncoated and self-assembled monolayer (SAM) coated silicon wafers. Several characterization techniques -AFM, ellipsometry, water contact angle, and fluorescence microscopy -were used to evaluate the protein-adsorbed layer. An uncoated silicon surface was most attractive to proteins, forming a 70 Å thick film from a solution of BSA at a concentration comparable to that in cell growth serum. Coating with the hydrophobic octadecyltrimethoxysilane (OTMS) SAM reduced protein adhesion by ~15%. In contrast, a hydrophilic N-(triethoxysilylpropyl)-O-polyethyleneoxide urethane (TESP) SAM inhibited protein adhesion by greater than 50%. Protein adhesion studies with cell growth sera containing complex mixtures of proteins paralleled the BSA adsorption studies, clearly identifying the TESP coated surface as a promising biocompatible coating.4

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Section: Introductionmentioning

confidence: 99%

Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Follstaedt¹,

Cheung²,

Gourley³

et al. 2000

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“…A method often used to anchor macromolecular units, such as proteins or enzymes, onto a solid surface is the functionalization of a self-assembled monolayer (SAM) of alkanethiols [see, for example, refs [15][16][17][18][19][20][21][22][23][24][25][26][27]. The success of SAMs is due to the simplicity of the experimental procedure to prepare the films, their reproducibility, and the possibility of creating a wide range of surfaces via the incorporation of different groups at the end of the alkyl chains.…”

Section: Introductionmentioning

confidence: 99%

Structural, Electrochemical, and Photophysical Properties of a Molecular Shuttle Attached to an Acid-Terminated Self-Assembled Monolayer

Cecchet¹,

Rudolf²,

Rapino³

et al. 2004

J. Phys. Chem. B

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Structural, electrochemical, and photophysical properties of a molecular shuttle attached to an acid-terminated self-assembled monolayer Cecchet, F; Rudolf, Petra; Rapino, S; Margotti, M; Paolucci, F; Baggerman, J; Kay, ER; Wong, JKY; Leigh, DA; Kay, Euan R. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. March 30, 2004; In Final Form: July 6, 2004 A benzylic amide macrocycle containing a pyridine moiety (macrocycle 2) and a related benzylic amide macrocycle-based molecular shuttle (naphthalimide rotaxane) with two pyridine moieties on the macrocyclic unit were grafted onto a self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid (11-MUA) on gold. X-ray photoelectron spectroscopy (XPS) indicates that the molecules are linked to the SAM by hydrogenbonding. Electrochemical investigations show that the self-assembled monolayer is densely packed and well ordered and allows the estimation of the average thickness of the SAM alone and of the SAM functionalized with either macrocycle 2 or with naphthalimide rotaxane. The estimated thickness values suggest that the 11-MUA chains in the SAM are tilted with respect to the surface normal, as expected for ordered and stable SAMs, whereas the rotaxane molecules are oriented with the thread parallel to the SAM surface and macrocycle plan preferentially perpendicular to the surface. The photophysical studies of the naphthalimide rotaxane grafted onto the SAM on gold demonstrated that fluorescence is partially quenched but still remains easily measurable because the presence of the SAM reduces the quenching effect of the metal substrate. Moreover, the photophysical analysis clearly indicates that the naphthalimide part of the rotaxane strongly interacts with the carboxylic groups of the SAM, in agreement with the orientation of the molecule obtained from the electrochemistry.

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“…The ability to validate the exact spatial location of immobilised protein and the immobilised quantity thereof on a surface is important and techniques such as surface plasmon resonance [17,18], ellipsometry [19], optical waveguide lightmode spectroscopy [20], and quartz crystal microbalance [21] have been previously reported. These techniques quantitatively measure the amount of protein immobilised on flat, planar surfaces such as glass, gold, and plastic substrates, but these methods cannot easily be utilised for macroporous monolithic materials, because of the huge disparity between the surface area of a monolith compared with a flat surface.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of photografted charged sites within polymer monoliths in capillary columns using contactless conductivity detection

Connolly

O'Shea

Clark

et al. 2007

J of Separation Science

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Original PaperEvaluation of photografted charged sites within polymer monoliths in capillary columns using contactless conductivity detection Capacitively coupled contactless conductivity detection (C 4 D) is presented as a novel and versatile means of visualising discrete zones of charged functional groups grafted onto polymer based monoliths. Monoliths were first formed within 100 lm UV-transparent fused silica capillaries. Photografting methods were subsequently used to graft a charged functional monomer, 2-acrylamido-2-methyl-1-propanesulfonic acid, onto discrete regions of the monolith using a photomask. Post-modification monolith evaluation involves scanning the C 4 D detector along the length of the monolith to obtain a profile of the exact spatial location of grafted charged functionalities with millimetre accuracy. The methodology was extended to the visualisation of several zones of immobilised protein (bovine serum albumin) using photografted azlactone groups to enable covalent attachment of the protein to the monolith at precise locations along its length. In addition, the extent of non-specific binding of protein to the ungrafted regions of the monolith due to hydrophobic interactions could be monitored as an increase in background conductivity of the stationary phase. Finally, the technique was cross-validated using digital photography in combination with a UV light source by immobilising green fluorescent protein in discrete zones and comparing the results obtained using both complementary techniques.

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The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver

Cited by 447 publications

References 167 publications

Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Structural, Electrochemical, and Photophysical Properties of a Molecular Shuttle Attached to an Acid-Terminated Self-Assembled Monolayer

Evaluation of photografted charged sites within polymer monoliths in capillary columns using contactless conductivity detection

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